Bile secretion is centrally regulated by C-type natriuretic peptide

Maria Eugenia Sabbatini, Marcelo S. Vatta, Cristina Vescina, José L. Castro, Belisario E. Fernández, Liliana G. Bianciotti

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

1. Current evidence supports that C-type natriuretic peptide (CNP) is the brain natriuretic peptide. Natriuretic peptide receptors and mRNA CNP have been reported in the liver and in discrete areas and nucleus of the central nervous system involved in the regulation of gastrointestinal physiology. In the present work, we sought to establish the role of CNP in the central regulation of bile secretion in the rat and to delineate the possible pathways and mechanisms involved. 2. To examine the role of CNP on bile secretion, the peptide was applied in the brain lateral ventricle (1, 10, and 100 ng/μL) and bile samples were collected every 15 min for 60 min. The role of the autonomic nervous system in CNP response was assessed by atropine or combined phentolamine and propranolol administration. 3. Centrally applied CNP diminished basal as well as bile salt-evoked bile flow in a dose-dependent manner. CNP reduced bile acid output as well as sodium and potassium excretion, supporting CNP effect on bile acid-dependent flow. CNP also decreased chloride excretion and increased bile pH. The excretion of total glutathione was not affected by centrally applied CNP suggesting that this peptide does not alter bile acid-independent flow. Neither parasympathetic nor sympathetic blockade abolished CNP inhibitory response on bile secretion. Mean arterial pressure and portal venous pressure were not modified by CNP. 4. Present findings show that centrally applied CNP modulates bile secretion in a dose-dependent fashion. CNP alkalinized bile and reduced bile acid-dependent flow without affecting bile acid-independent flow. The inhibitory response of CNP on bile secretion was not mediated by the autonomic nervous system. Present findings give further support to the role of CNP as the brain natriuretic peptide.

Original languageEnglish (US)
Pages (from-to)755-770
Number of pages16
JournalCellular and Molecular Neurobiology
Volume22
Issue number5-6
DOIs
StatePublished - Dec 1 2002
Externally publishedYes

Fingerprint

C-Type Natriuretic Peptide
Bile
Bile Acids and Salts
Neurology
Brain Natriuretic Peptide
Autonomic Nervous System
Natriuretic Agents
Portal Pressure
Natriuretic Peptides
Peptides
Peptide Receptors

Keywords

  • Bile flow
  • Bile salts
  • CNP
  • Glutathione
  • Portal venous pressure

ASJC Scopus subject areas

  • Neuroscience(all)
  • Clinical Biochemistry
  • Cell Biology
  • Genetics

Cite this

Sabbatini, M. E., Vatta, M. S., Vescina, C., Castro, J. L., Fernández, B. E., & Bianciotti, L. G. (2002). Bile secretion is centrally regulated by C-type natriuretic peptide. Cellular and Molecular Neurobiology, 22(5-6), 755-770. https://doi.org/10.1023/A:1021813225723

Bile secretion is centrally regulated by C-type natriuretic peptide. / Sabbatini, Maria Eugenia; Vatta, Marcelo S.; Vescina, Cristina; Castro, José L.; Fernández, Belisario E.; Bianciotti, Liliana G.

In: Cellular and Molecular Neurobiology, Vol. 22, No. 5-6, 01.12.2002, p. 755-770.

Research output: Contribution to journalArticle

Sabbatini, ME, Vatta, MS, Vescina, C, Castro, JL, Fernández, BE & Bianciotti, LG 2002, 'Bile secretion is centrally regulated by C-type natriuretic peptide', Cellular and Molecular Neurobiology, vol. 22, no. 5-6, pp. 755-770. https://doi.org/10.1023/A:1021813225723
Sabbatini, Maria Eugenia ; Vatta, Marcelo S. ; Vescina, Cristina ; Castro, José L. ; Fernández, Belisario E. ; Bianciotti, Liliana G. / Bile secretion is centrally regulated by C-type natriuretic peptide. In: Cellular and Molecular Neurobiology. 2002 ; Vol. 22, No. 5-6. pp. 755-770.
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