Biocompatible synthesis of peptide capped copper nanoparticles and their biological effect on tumor cells

Mayur Valodkar, Ravirajsinh N. Jadeja, Menaka C. Thounaojam, Ranjitsinh V. Devkar, Sonal Thakore

Research output: Contribution to journalArticlepeer-review

75 Scopus citations

Abstract

Extracellular synthesis of copper nanoparticles was carried out using stem latex of a medicinally important plant, Euphorbia nivulia. The nanoparticles were stabilized and subsequently capped by peptides and terpenoids present within the latex. The protein capping is a promising biocompatible vehicle for destruction of tumor/cancer cells. The cytotoxicity potential of the plant protein capped nanoparticles was evaluated using various parameters like MTT cell viability assay and extracellular lactate dehydrogenase (LDH) release in cancer cell line. Other parameters that determine the oxidative stress viz., reactive oxygen species (ROS) generation, intracellular reduced glutathione (GSH), malondialdehyde (MDA), superoxide generation and acridine orange/ethidium bromide staining were also investigated. The present study led to the conclusion that copper nanoparticles are toxic to A549 cells in a dose dependent manner. The non-toxic aqueous formulation of latex capped copper nanoparticles can be directly used for administration/in vivo delivery of nanoparticles for cancer therapy.

Original languageEnglish (US)
Pages (from-to)83-89
Number of pages7
JournalMaterials Chemistry and Physics
Volume128
Issue number1-2
DOIs
StatePublished - Jul 15 2011
Externally publishedYes

Keywords

  • A. Biomaterials
  • A. Nanostructures
  • C. Electron microscopy
  • C. Fourier transform infrared spectroscopy (FTIR)

ASJC Scopus subject areas

  • Materials Science(all)
  • Condensed Matter Physics

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