Blue light activates phase 2 response proteins and slows growth of A431 epidermoid carcinoma xenografts

Alpesh D. Patel, Shaun Rotenberg, Regina L W Messer, John C. Wataha, Kalu U.E. Ogbureke, Veronica V. Mccloud, Petra Lockwood, Stephen Hsu, Jill B. Lewis

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Background: Recent studies suggest that light in the UVA range (320-400 nm) activates signaling pathways that are anti-inflammatory, antioxidative and play a critical role in protection against cancer. These effects have been attributed to NF-E2-related factor (NRF2)-mediated upregulation of 'phase 2' genes that neutralize oxidative stress and metabolize electrophiles. We had previously shown that small doses of blue light (400-500 nm) had selective toxicity for cultured oral tumor cells and increased levels of peroxiredoxin phase 2 proteins, which led to our hypothesis that blue light activates NRF2 signaling. Materials and Methods: A431 epidermoid carcinoma cells were treated in culture and as nude mouse xenografts with doses of blue light. Cell lysates and tumor samples were tested for NRF2 activation, and for markers of proliferation and oxidative stress. Results: Blue light activated the phase 2 response in cultured A431 cells and reduced their viability dose dependently. Light treatment of tumors reduced tumor growth, and levels of proliferating cell nuclear antigen (PCNA), and oxidized proteins. Discussion: Cellular responses to these light energies are worth further study and may provide therapeutic interventions for inflammation and cancer.

Original languageEnglish (US)
Pages (from-to)6305-6313
Number of pages9
JournalAnticancer Research
Volume34
Issue number11
StatePublished - Jan 1 2014

Fingerprint

Heterografts
Squamous Cell Carcinoma
Light
Growth
Proteins
Neoplasms
Oxidative Stress
Cultured Tumor Cells
Peroxiredoxins
Proliferating Cell Nuclear Antigen
Nude Mice
Cultured Cells
Anti-Inflammatory Agents
Up-Regulation
Inflammation
Genes

Keywords

  • Blue light
  • Heme oxygenase
  • NRF2
  • Oxidative stress
  • Xenograft

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Patel, A. D., Rotenberg, S., Messer, R. L. W., Wataha, J. C., Ogbureke, K. U. E., Mccloud, V. V., ... Lewis, J. B. (2014). Blue light activates phase 2 response proteins and slows growth of A431 epidermoid carcinoma xenografts. Anticancer Research, 34(11), 6305-6313.

Blue light activates phase 2 response proteins and slows growth of A431 epidermoid carcinoma xenografts. / Patel, Alpesh D.; Rotenberg, Shaun; Messer, Regina L W; Wataha, John C.; Ogbureke, Kalu U.E.; Mccloud, Veronica V.; Lockwood, Petra; Hsu, Stephen; Lewis, Jill B.

In: Anticancer Research, Vol. 34, No. 11, 01.01.2014, p. 6305-6313.

Research output: Contribution to journalArticle

Patel, AD, Rotenberg, S, Messer, RLW, Wataha, JC, Ogbureke, KUE, Mccloud, VV, Lockwood, P, Hsu, S & Lewis, JB 2014, 'Blue light activates phase 2 response proteins and slows growth of A431 epidermoid carcinoma xenografts', Anticancer Research, vol. 34, no. 11, pp. 6305-6313.
Patel AD, Rotenberg S, Messer RLW, Wataha JC, Ogbureke KUE, Mccloud VV et al. Blue light activates phase 2 response proteins and slows growth of A431 epidermoid carcinoma xenografts. Anticancer Research. 2014 Jan 1;34(11):6305-6313.
Patel, Alpesh D. ; Rotenberg, Shaun ; Messer, Regina L W ; Wataha, John C. ; Ogbureke, Kalu U.E. ; Mccloud, Veronica V. ; Lockwood, Petra ; Hsu, Stephen ; Lewis, Jill B. / Blue light activates phase 2 response proteins and slows growth of A431 epidermoid carcinoma xenografts. In: Anticancer Research. 2014 ; Vol. 34, No. 11. pp. 6305-6313.
@article{54244846209d4462a54dbe83b7774457,
title = "Blue light activates phase 2 response proteins and slows growth of A431 epidermoid carcinoma xenografts",
abstract = "Background: Recent studies suggest that light in the UVA range (320-400 nm) activates signaling pathways that are anti-inflammatory, antioxidative and play a critical role in protection against cancer. These effects have been attributed to NF-E2-related factor (NRF2)-mediated upregulation of 'phase 2' genes that neutralize oxidative stress and metabolize electrophiles. We had previously shown that small doses of blue light (400-500 nm) had selective toxicity for cultured oral tumor cells and increased levels of peroxiredoxin phase 2 proteins, which led to our hypothesis that blue light activates NRF2 signaling. Materials and Methods: A431 epidermoid carcinoma cells were treated in culture and as nude mouse xenografts with doses of blue light. Cell lysates and tumor samples were tested for NRF2 activation, and for markers of proliferation and oxidative stress. Results: Blue light activated the phase 2 response in cultured A431 cells and reduced their viability dose dependently. Light treatment of tumors reduced tumor growth, and levels of proliferating cell nuclear antigen (PCNA), and oxidized proteins. Discussion: Cellular responses to these light energies are worth further study and may provide therapeutic interventions for inflammation and cancer.",
keywords = "Blue light, Heme oxygenase, NRF2, Oxidative stress, Xenograft",
author = "Patel, {Alpesh D.} and Shaun Rotenberg and Messer, {Regina L W} and Wataha, {John C.} and Ogbureke, {Kalu U.E.} and Mccloud, {Veronica V.} and Petra Lockwood and Stephen Hsu and Lewis, {Jill B.}",
year = "2014",
month = "1",
day = "1",
language = "English (US)",
volume = "34",
pages = "6305--6313",
journal = "Anticancer Research",
issn = "0250-7005",
publisher = "International Institute of Anticancer Research",
number = "11",

}

TY - JOUR

T1 - Blue light activates phase 2 response proteins and slows growth of A431 epidermoid carcinoma xenografts

AU - Patel, Alpesh D.

AU - Rotenberg, Shaun

AU - Messer, Regina L W

AU - Wataha, John C.

AU - Ogbureke, Kalu U.E.

AU - Mccloud, Veronica V.

AU - Lockwood, Petra

AU - Hsu, Stephen

AU - Lewis, Jill B.

PY - 2014/1/1

Y1 - 2014/1/1

N2 - Background: Recent studies suggest that light in the UVA range (320-400 nm) activates signaling pathways that are anti-inflammatory, antioxidative and play a critical role in protection against cancer. These effects have been attributed to NF-E2-related factor (NRF2)-mediated upregulation of 'phase 2' genes that neutralize oxidative stress and metabolize electrophiles. We had previously shown that small doses of blue light (400-500 nm) had selective toxicity for cultured oral tumor cells and increased levels of peroxiredoxin phase 2 proteins, which led to our hypothesis that blue light activates NRF2 signaling. Materials and Methods: A431 epidermoid carcinoma cells were treated in culture and as nude mouse xenografts with doses of blue light. Cell lysates and tumor samples were tested for NRF2 activation, and for markers of proliferation and oxidative stress. Results: Blue light activated the phase 2 response in cultured A431 cells and reduced their viability dose dependently. Light treatment of tumors reduced tumor growth, and levels of proliferating cell nuclear antigen (PCNA), and oxidized proteins. Discussion: Cellular responses to these light energies are worth further study and may provide therapeutic interventions for inflammation and cancer.

AB - Background: Recent studies suggest that light in the UVA range (320-400 nm) activates signaling pathways that are anti-inflammatory, antioxidative and play a critical role in protection against cancer. These effects have been attributed to NF-E2-related factor (NRF2)-mediated upregulation of 'phase 2' genes that neutralize oxidative stress and metabolize electrophiles. We had previously shown that small doses of blue light (400-500 nm) had selective toxicity for cultured oral tumor cells and increased levels of peroxiredoxin phase 2 proteins, which led to our hypothesis that blue light activates NRF2 signaling. Materials and Methods: A431 epidermoid carcinoma cells were treated in culture and as nude mouse xenografts with doses of blue light. Cell lysates and tumor samples were tested for NRF2 activation, and for markers of proliferation and oxidative stress. Results: Blue light activated the phase 2 response in cultured A431 cells and reduced their viability dose dependently. Light treatment of tumors reduced tumor growth, and levels of proliferating cell nuclear antigen (PCNA), and oxidized proteins. Discussion: Cellular responses to these light energies are worth further study and may provide therapeutic interventions for inflammation and cancer.

KW - Blue light

KW - Heme oxygenase

KW - NRF2

KW - Oxidative stress

KW - Xenograft

UR - http://www.scopus.com/inward/record.url?scp=84916201936&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84916201936&partnerID=8YFLogxK

M3 - Article

C2 - 25368229

AN - SCOPUS:84916201936

VL - 34

SP - 6305

EP - 6313

JO - Anticancer Research

JF - Anticancer Research

SN - 0250-7005

IS - 11

ER -