Bone generation in the reconstruction of a critical size calvarial defect in an experimental model

Yong Chen Por, Carlos Raul Barceló, Kenneth E. Salyer, David G. Genecov, Karen Troxel, El Gendler, Mohammed Elsayed Elsalanty, Lynne A. Opperman

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Objective: This study was designed to investigate the optimal combination of known osteogenic biomaterials with shape conforming struts to achieve calvarial vault reconstruction, using a canine model. Methods: Eighteen adolescent beagles were divided equally into 6 groups. A critical size defect of 6 x 2 cm traversed the sagittal suture. The biomaterials used for calvarial reconstruction were demineralised perforated bone matrix (DBM), recombinant human bone morphogenetic protein-2 (rhBMP2) and autogenous platelet-rich plasma (PRP). The struts used were cobalt chrome (metal) or resorbable plate. The groupings were as follows: 1) DBM + metal, 2) DBM + PRP + metal, 3) DBM + PRP + resorbable plate, 4) DBM + rhBMP2 + metal, 5) DBM + rhBMP2 + PRP + metal, and 6) DBM + rhBMP2 + resorbable plate. Animals were euthanised at 3 months post-surgery. There was no mortality or major complications. Analysis was performed macroscopically, histologically, and with computed tomography (CT). Results: There was complete bony regeneration in the rhBMP2 groups only. Non-rhBMP2 groups had minimal bony ingrowth from the defect edges and on the dural surface, a finding confirmed by CT scan and histology. PRP did not enhance bone regeneration. Shape conformation was good with both metal and resorbable plate. Conclusion: rhBMP2 but not PRP accelerated calvarial regeneration in 3 months. The DBM in the rhBMP2 groups were substituted by new trabecular bone. Shape molding was good with both metal and resorbable plate.

Original languageEnglish (US)
Pages (from-to)911-919
Number of pages9
JournalAnnals of the Academy of Medicine Singapore
Volume36
Issue number11
StatePublished - Nov 1 2007
Externally publishedYes

Fingerprint

Bone Matrix
Platelet-Rich Plasma
Theoretical Models
Metals
Bone and Bones
Biocompatible Materials
Regeneration
Tomography
Bone Regeneration
Cobalt
recombinant human bone morphogenetic protein-2
Sutures
Canidae
Histology
Mortality

Keywords

  • Cranial vault reconstruction
  • Critical size calvarial defect
  • Metal struts
  • Resorbable plates
  • rhBMP2

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Por, Y. C., Barceló, C. R., Salyer, K. E., Genecov, D. G., Troxel, K., Gendler, E., ... Opperman, L. A. (2007). Bone generation in the reconstruction of a critical size calvarial defect in an experimental model. Annals of the Academy of Medicine Singapore, 36(11), 911-919.

Bone generation in the reconstruction of a critical size calvarial defect in an experimental model. / Por, Yong Chen; Barceló, Carlos Raul; Salyer, Kenneth E.; Genecov, David G.; Troxel, Karen; Gendler, El; Elsalanty, Mohammed Elsayed; Opperman, Lynne A.

In: Annals of the Academy of Medicine Singapore, Vol. 36, No. 11, 01.11.2007, p. 911-919.

Research output: Contribution to journalArticle

Por, YC, Barceló, CR, Salyer, KE, Genecov, DG, Troxel, K, Gendler, E, Elsalanty, ME & Opperman, LA 2007, 'Bone generation in the reconstruction of a critical size calvarial defect in an experimental model', Annals of the Academy of Medicine Singapore, vol. 36, no. 11, pp. 911-919.
Por YC, Barceló CR, Salyer KE, Genecov DG, Troxel K, Gendler E et al. Bone generation in the reconstruction of a critical size calvarial defect in an experimental model. Annals of the Academy of Medicine Singapore. 2007 Nov 1;36(11):911-919.
Por, Yong Chen ; Barceló, Carlos Raul ; Salyer, Kenneth E. ; Genecov, David G. ; Troxel, Karen ; Gendler, El ; Elsalanty, Mohammed Elsayed ; Opperman, Lynne A. / Bone generation in the reconstruction of a critical size calvarial defect in an experimental model. In: Annals of the Academy of Medicine Singapore. 2007 ; Vol. 36, No. 11. pp. 911-919.
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abstract = "Objective: This study was designed to investigate the optimal combination of known osteogenic biomaterials with shape conforming struts to achieve calvarial vault reconstruction, using a canine model. Methods: Eighteen adolescent beagles were divided equally into 6 groups. A critical size defect of 6 x 2 cm traversed the sagittal suture. The biomaterials used for calvarial reconstruction were demineralised perforated bone matrix (DBM), recombinant human bone morphogenetic protein-2 (rhBMP2) and autogenous platelet-rich plasma (PRP). The struts used were cobalt chrome (metal) or resorbable plate. The groupings were as follows: 1) DBM + metal, 2) DBM + PRP + metal, 3) DBM + PRP + resorbable plate, 4) DBM + rhBMP2 + metal, 5) DBM + rhBMP2 + PRP + metal, and 6) DBM + rhBMP2 + resorbable plate. Animals were euthanised at 3 months post-surgery. There was no mortality or major complications. Analysis was performed macroscopically, histologically, and with computed tomography (CT). Results: There was complete bony regeneration in the rhBMP2 groups only. Non-rhBMP2 groups had minimal bony ingrowth from the defect edges and on the dural surface, a finding confirmed by CT scan and histology. PRP did not enhance bone regeneration. Shape conformation was good with both metal and resorbable plate. Conclusion: rhBMP2 but not PRP accelerated calvarial regeneration in 3 months. The DBM in the rhBMP2 groups were substituted by new trabecular bone. Shape molding was good with both metal and resorbable plate.",
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