Bone marrow-derived stromal cells (BMSCs) interact with fibroblasts in accelerating wound healing

Deborah Dulchavsky, Xiaohua Gao, Yong Bo Liu, Dorrah Deeb, Ali Syed Arbab, Kevin McIntosh, Scott A. Dulchavsky, Subhash C. Gautam

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Bone marrow-derived stromal cells (BMSCs) exhibit extraordinary degree of plasticity and growth factor repertoire for which they have been investigated for repair and regeneration of damaged tissues, but have not been adequately examined for wound healing. The ability of BMSCs to accelerate healing of surgically inflicted cutaneous and fascial wounds was tested in vivo in rats and in vitro using a fibroblast monolayer wound model. Intravenous treatment with BMSCs augmented healing of both cutaneous and fascial wounds as determined by an increase in the biomechanical strength of wounds. In vitro experiments showed that incorporation of BMSCs in fibroblast monolayers accelerates the closure of mechanically disrupted monolayers, which was attributed to the enhanced migration of fibroblasts onto the denuded surfaces. Furthermore, culture medium conditioned by activated BMSCs promoted the closure of defects in monolayers and enhanced the proliferation/growth and directional migration (chemotaxis) of fibroblasts. This study demonstrates that BMSCs significantly augment healing of cutaneous and fascial wounds in vivo at least in part through interaction with fibroblasts in which BMSCs promote growth and chemotaxis of fibroblasts.

Original languageEnglish (US)
Pages (from-to)270-279
Number of pages10
JournalJournal of Investigative Surgery
Volume21
Issue number5
DOIs
StatePublished - Dec 1 2008
Externally publishedYes

Fingerprint

Mesenchymal Stromal Cells
Wound Healing
Fibroblasts
Wounds and Injuries
Chemotaxis
Skin
Aptitude
Conditioned Culture Medium
Growth
Regeneration
Intercellular Signaling Peptides and Proteins

Keywords

  • Cell migration
  • Fibroblasts
  • Mesenchymal stem cells
  • Wound closure
  • Wound healing

ASJC Scopus subject areas

  • Surgery

Cite this

Bone marrow-derived stromal cells (BMSCs) interact with fibroblasts in accelerating wound healing. / Dulchavsky, Deborah; Gao, Xiaohua; Liu, Yong Bo; Deeb, Dorrah; Arbab, Ali Syed; McIntosh, Kevin; Dulchavsky, Scott A.; Gautam, Subhash C.

In: Journal of Investigative Surgery, Vol. 21, No. 5, 01.12.2008, p. 270-279.

Research output: Contribution to journalArticle

Dulchavsky, D, Gao, X, Liu, YB, Deeb, D, Arbab, AS, McIntosh, K, Dulchavsky, SA & Gautam, SC 2008, 'Bone marrow-derived stromal cells (BMSCs) interact with fibroblasts in accelerating wound healing', Journal of Investigative Surgery, vol. 21, no. 5, pp. 270-279. https://doi.org/10.1080/08941930802216831
Dulchavsky, Deborah ; Gao, Xiaohua ; Liu, Yong Bo ; Deeb, Dorrah ; Arbab, Ali Syed ; McIntosh, Kevin ; Dulchavsky, Scott A. ; Gautam, Subhash C. / Bone marrow-derived stromal cells (BMSCs) interact with fibroblasts in accelerating wound healing. In: Journal of Investigative Surgery. 2008 ; Vol. 21, No. 5. pp. 270-279.
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