Abstract
Brainbow is a genetic cell-labeling technique where hundreds of different hues can be generated by stochastic and combinatorial expression of a few spectrally distinct fluorescent proteins. Unique color profiles can be used as cellular identification tags for multiple applications such as tracing axons through the nervous system, following individual cells during development, or analyzing cell lineage. In recent years, Brainbow and other combinatorial expression strategies have expanded from the mouse nervous system to other model organisms and a wide variety of tissues. Particularly exciting is the application of Brainbow in lineage tracing, where this technique has been instrumental in parsing out complex cellular relationships during organogenesis. Here we review recent findings, new technical improvements, and exciting potential genetic and genomic applications for harnessing this colorful technique in anatomical, developmental, and genetic studies.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 293-306 |
| Number of pages | 14 |
| Journal | Genetics |
| Volume | 199 |
| Issue number | 2 |
| DOIs | |
| State | Published - Feb 1 2014 |
Fingerprint
Keywords
- Clonal analysis
- Fluorescence microscopy
- In vivo imaging
- Lineage tracing
- Neural circuitry
ASJC Scopus subject areas
- Genetics
Cite this
Brainbow : New resources and emerging biological applications for multicolor genetic labeling and analysis. / Weissman, Tamily A.; Pan, Y. Albert.
In: Genetics, Vol. 199, No. 2, 01.02.2014, p. 293-306.Research output: Contribution to journal › Review article
}
TY - JOUR
T1 - Brainbow
T2 - New resources and emerging biological applications for multicolor genetic labeling and analysis
AU - Weissman, Tamily A.
AU - Pan, Y. Albert
PY - 2014/2/1
Y1 - 2014/2/1
N2 - Brainbow is a genetic cell-labeling technique where hundreds of different hues can be generated by stochastic and combinatorial expression of a few spectrally distinct fluorescent proteins. Unique color profiles can be used as cellular identification tags for multiple applications such as tracing axons through the nervous system, following individual cells during development, or analyzing cell lineage. In recent years, Brainbow and other combinatorial expression strategies have expanded from the mouse nervous system to other model organisms and a wide variety of tissues. Particularly exciting is the application of Brainbow in lineage tracing, where this technique has been instrumental in parsing out complex cellular relationships during organogenesis. Here we review recent findings, new technical improvements, and exciting potential genetic and genomic applications for harnessing this colorful technique in anatomical, developmental, and genetic studies.
AB - Brainbow is a genetic cell-labeling technique where hundreds of different hues can be generated by stochastic and combinatorial expression of a few spectrally distinct fluorescent proteins. Unique color profiles can be used as cellular identification tags for multiple applications such as tracing axons through the nervous system, following individual cells during development, or analyzing cell lineage. In recent years, Brainbow and other combinatorial expression strategies have expanded from the mouse nervous system to other model organisms and a wide variety of tissues. Particularly exciting is the application of Brainbow in lineage tracing, where this technique has been instrumental in parsing out complex cellular relationships during organogenesis. Here we review recent findings, new technical improvements, and exciting potential genetic and genomic applications for harnessing this colorful technique in anatomical, developmental, and genetic studies.
KW - Clonal analysis
KW - Fluorescence microscopy
KW - In vivo imaging
KW - Lineage tracing
KW - Neural circuitry
UR - http://www.scopus.com/inward/record.url?scp=84936752009&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84936752009&partnerID=8YFLogxK
U2 - 10.1534/genetics.114.172510
DO - 10.1534/genetics.114.172510
M3 - Review article
C2 - 25657347
AN - SCOPUS:84936752009
VL - 199
SP - 293
EP - 306
JO - Genetics
JF - Genetics
SN - 0016-6731
IS - 2
ER -