Calcium sequestration by subcellular fractions isolated from vascular smooth muscle

Effect of cyclic nucleotides and prostaglandins

R Clinton Webb, Ramesh C. Bhalla

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Microsomal vesicles and mitochondria derived from aortae of rat accumulate calcium in the presence of adenosine triphosphate and magnesium. The rate and extent of energy-linked calcium sequestration by microsomal vesicles were found to exhibit hydrogen ion and temperature dependency and nucleoside phosphate specificity. Calcium uptake (in the presence of oxalate) showed preference for nucleosides in the order ATP>ADP>GTP>UTP>ITP. Calcium accumulation by the subcellular fractions was also found to be dependent upon the concentration of calcium in the incubation medium. Cyclic AMP stimulated microsomal calcium binding while prostaglandins F and E2 had no specific effect. These agents did not alter the calcium sequestering mechanisms of the mitochondria. These data suggest that the vesicular and mitochondrial fraction may constitute an important factor in the regulation of calcium availability to the contractile mechanism of vascular smooth muscle.

Original languageEnglish (US)
Pages (from-to)145-157
Number of pages13
JournalJournal of Molecular and Cellular Cardiology
Volume8
Issue number2
DOIs
StatePublished - Jan 1 1976
Externally publishedYes

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Subcellular Fractions
Cyclic Nucleotides
Vascular Smooth Muscle
Prostaglandins
Calcium
Nucleosides
Mitochondria
Adenosine Triphosphate
Inosine Triphosphate
Uridine Triphosphate
Dinoprost
Oxalates
Guanosine Triphosphate
Dinoprostone
Cyclic AMP
Adenosine Diphosphate
Aorta
Protons
Phosphates
Temperature

Keywords

  • ATPase
  • Calcium
  • Cyclic AMP
  • Microsomal vesicles
  • Mitochondria
  • Prostaglandins
  • Vascular smooth muscle

ASJC Scopus subject areas

  • Molecular Biology
  • Cardiology and Cardiovascular Medicine

Cite this

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title = "Calcium sequestration by subcellular fractions isolated from vascular smooth muscle: Effect of cyclic nucleotides and prostaglandins",
abstract = "Microsomal vesicles and mitochondria derived from aortae of rat accumulate calcium in the presence of adenosine triphosphate and magnesium. The rate and extent of energy-linked calcium sequestration by microsomal vesicles were found to exhibit hydrogen ion and temperature dependency and nucleoside phosphate specificity. Calcium uptake (in the presence of oxalate) showed preference for nucleosides in the order ATP>ADP>GTP>UTP>ITP. Calcium accumulation by the subcellular fractions was also found to be dependent upon the concentration of calcium in the incubation medium. Cyclic AMP stimulated microsomal calcium binding while prostaglandins F2α and E2 had no specific effect. These agents did not alter the calcium sequestering mechanisms of the mitochondria. These data suggest that the vesicular and mitochondrial fraction may constitute an important factor in the regulation of calcium availability to the contractile mechanism of vascular smooth muscle.",
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T2 - Effect of cyclic nucleotides and prostaglandins

AU - Webb, R Clinton

AU - Bhalla, Ramesh C.

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N2 - Microsomal vesicles and mitochondria derived from aortae of rat accumulate calcium in the presence of adenosine triphosphate and magnesium. The rate and extent of energy-linked calcium sequestration by microsomal vesicles were found to exhibit hydrogen ion and temperature dependency and nucleoside phosphate specificity. Calcium uptake (in the presence of oxalate) showed preference for nucleosides in the order ATP>ADP>GTP>UTP>ITP. Calcium accumulation by the subcellular fractions was also found to be dependent upon the concentration of calcium in the incubation medium. Cyclic AMP stimulated microsomal calcium binding while prostaglandins F2α and E2 had no specific effect. These agents did not alter the calcium sequestering mechanisms of the mitochondria. These data suggest that the vesicular and mitochondrial fraction may constitute an important factor in the regulation of calcium availability to the contractile mechanism of vascular smooth muscle.

AB - Microsomal vesicles and mitochondria derived from aortae of rat accumulate calcium in the presence of adenosine triphosphate and magnesium. The rate and extent of energy-linked calcium sequestration by microsomal vesicles were found to exhibit hydrogen ion and temperature dependency and nucleoside phosphate specificity. Calcium uptake (in the presence of oxalate) showed preference for nucleosides in the order ATP>ADP>GTP>UTP>ITP. Calcium accumulation by the subcellular fractions was also found to be dependent upon the concentration of calcium in the incubation medium. Cyclic AMP stimulated microsomal calcium binding while prostaglandins F2α and E2 had no specific effect. These agents did not alter the calcium sequestering mechanisms of the mitochondria. These data suggest that the vesicular and mitochondrial fraction may constitute an important factor in the regulation of calcium availability to the contractile mechanism of vascular smooth muscle.

KW - ATPase

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KW - Cyclic AMP

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KW - Mitochondria

KW - Prostaglandins

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