Abstract
Microsomal vesicles and mitochondria derived from aortae of rat accumulate calcium in the presence of adenosine triphosphate and magnesium. The rate and extent of energy-linked calcium sequestration by microsomal vesicles were found to exhibit hydrogen ion and temperature dependency and nucleoside phosphate specificity. Calcium uptake (in the presence of oxalate) showed preference for nucleosides in the order ATP>ADP>GTP>UTP>ITP. Calcium accumulation by the subcellular fractions was also found to be dependent upon the concentration of calcium in the incubation medium. Cyclic AMP stimulated microsomal calcium binding while prostaglandins F2α and E2 had no specific effect. These agents did not alter the calcium sequestering mechanisms of the mitochondria. These data suggest that the vesicular and mitochondrial fraction may constitute an important factor in the regulation of calcium availability to the contractile mechanism of vascular smooth muscle.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 145-157 |
| Number of pages | 13 |
| Journal | Journal of Molecular and Cellular Cardiology |
| Volume | 8 |
| Issue number | 2 |
| DOIs | |
| State | Published - Jan 1 1976 |
| Externally published | Yes |
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Keywords
- ATPase
- Calcium
- Cyclic AMP
- Microsomal vesicles
- Mitochondria
- Prostaglandins
- Vascular smooth muscle
ASJC Scopus subject areas
- Molecular Biology
- Cardiology and Cardiovascular Medicine
Cite this
Calcium sequestration by subcellular fractions isolated from vascular smooth muscle : Effect of cyclic nucleotides and prostaglandins. / Webb, R Clinton; Bhalla, Ramesh C.
In: Journal of Molecular and Cellular Cardiology, Vol. 8, No. 2, 01.01.1976, p. 145-157.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Calcium sequestration by subcellular fractions isolated from vascular smooth muscle
T2 - Effect of cyclic nucleotides and prostaglandins
AU - Webb, R Clinton
AU - Bhalla, Ramesh C.
PY - 1976/1/1
Y1 - 1976/1/1
N2 - Microsomal vesicles and mitochondria derived from aortae of rat accumulate calcium in the presence of adenosine triphosphate and magnesium. The rate and extent of energy-linked calcium sequestration by microsomal vesicles were found to exhibit hydrogen ion and temperature dependency and nucleoside phosphate specificity. Calcium uptake (in the presence of oxalate) showed preference for nucleosides in the order ATP>ADP>GTP>UTP>ITP. Calcium accumulation by the subcellular fractions was also found to be dependent upon the concentration of calcium in the incubation medium. Cyclic AMP stimulated microsomal calcium binding while prostaglandins F2α and E2 had no specific effect. These agents did not alter the calcium sequestering mechanisms of the mitochondria. These data suggest that the vesicular and mitochondrial fraction may constitute an important factor in the regulation of calcium availability to the contractile mechanism of vascular smooth muscle.
AB - Microsomal vesicles and mitochondria derived from aortae of rat accumulate calcium in the presence of adenosine triphosphate and magnesium. The rate and extent of energy-linked calcium sequestration by microsomal vesicles were found to exhibit hydrogen ion and temperature dependency and nucleoside phosphate specificity. Calcium uptake (in the presence of oxalate) showed preference for nucleosides in the order ATP>ADP>GTP>UTP>ITP. Calcium accumulation by the subcellular fractions was also found to be dependent upon the concentration of calcium in the incubation medium. Cyclic AMP stimulated microsomal calcium binding while prostaglandins F2α and E2 had no specific effect. These agents did not alter the calcium sequestering mechanisms of the mitochondria. These data suggest that the vesicular and mitochondrial fraction may constitute an important factor in the regulation of calcium availability to the contractile mechanism of vascular smooth muscle.
KW - ATPase
KW - Calcium
KW - Cyclic AMP
KW - Microsomal vesicles
KW - Mitochondria
KW - Prostaglandins
KW - Vascular smooth muscle
UR - http://www.scopus.com/inward/record.url?scp=0017293444&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0017293444&partnerID=8YFLogxK
U2 - 10.1016/0022-2828(76)90026-2
DO - 10.1016/0022-2828(76)90026-2
M3 - Article
VL - 8
SP - 145
EP - 157
JO - Journal of Molecular and Cellular Cardiology
JF - Journal of Molecular and Cellular Cardiology
SN - 0022-2828
IS - 2
ER -