Calcyon, a novel partner of clathrin light chain, stimulates clathrin-mediated endocytosis

Jiping Xiao, Rujuan Dai, Laszlo Negyessy, Clare M Bergson

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

In the central nervous system, clathrin-mediated endocytosis is crucial for efficient synaptic transmission. Clathrin-coated vesicle assembly and disassembly is regulated by some 30 adaptor and accessory proteins, most of which interact with clathrin heavy chain. Using the calcyon cytosolic domain as bait, we isolated clathrin light chain in a yeast two-hybrid screen. The interaction domain was mapped to the heavy chain binding domain and C-terminal regions of light chain. Further, the addition of the calcyon C terminus stimulated clathrin self-assembly in a dose-dependent fashion. Calcyon, which is a single transmembrane protein predominantly expressed in brain, localized to vesicular compartments within pre- and postsynaptic structures. There was a high degree of overlap in the distribution of LC and calcyon in neuronal dendrites, spines, and cell bodies. Co-immunoprecipitation studies further suggested an association of calcyon with the clathrin-mediated endocytic machinery. Compared with controls, HEK293 cells overexpressing calcyon exhibited significantly enhanced transferrin uptake but equivalent levels of recycling. Conversely, transferrin uptake was largely abolished in neocortical neurons obtained from mice homozygous for a calcyon null allele, whereas recycling proceeded at wild type levels. Collectively, these data indicate a role for calcyon in clathrin-mediated endocytosis in brain.

Original languageEnglish (US)
Pages (from-to)15182-15193
Number of pages12
JournalJournal of Biological Chemistry
Volume281
Issue number22
DOIs
StatePublished - Jun 2 2006

Fingerprint

Clathrin Light Chains
Clathrin
Endocytosis
Recycling
Transferrin
Brain
Clathrin Heavy Chains
Clathrin-Coated Vesicles
calcyon
HEK293 Cells
Accessories
Neurology
Dendrites
Immunoprecipitation
Synaptic Transmission
Yeast
Self assembly
Neurons
Machinery
Proteins

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Calcyon, a novel partner of clathrin light chain, stimulates clathrin-mediated endocytosis. / Xiao, Jiping; Dai, Rujuan; Negyessy, Laszlo; Bergson, Clare M.

In: Journal of Biological Chemistry, Vol. 281, No. 22, 02.06.2006, p. 15182-15193.

Research output: Contribution to journalArticle

Xiao, Jiping ; Dai, Rujuan ; Negyessy, Laszlo ; Bergson, Clare M. / Calcyon, a novel partner of clathrin light chain, stimulates clathrin-mediated endocytosis. In: Journal of Biological Chemistry. 2006 ; Vol. 281, No. 22. pp. 15182-15193.
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