Calpain is required for the rapid, calcium-dependent repair of wounded plasma membrane

Ronald L. Mellgren, Wenli Zhang, Katsuya Miyake, Paul L McNeil

Research output: Contribution to journalArticle

84 Citations (Scopus)

Abstract

Mammalian cells require extracellular calcium ion to undergo rapid plasma membrane repair seconds after mechanical damage. Utilizing transformed fibroblasts from calpain small subunit knock-out (Capns1 -/- ) mouse embryos, we now show that the heterodimeric, typical subclass of calpains is required for calcium-mediated survival after plasma membrane damage caused by scraping a cell monolayer. Survival of scrape-damaged Capns1 -/- cells was unaffected by calcium in the scraping medium, whereas more Capns1 +/+ cells survived when calcium was present. Calcium-mediated survival was increased when Capns1 -/- cells were scraped in the presence of purified m- or μ-calpain. Survival rates of scraped Capns1 +/+ , HFL-1, or Chinese hamster ovary cells were decreased by the calpain inhibitor, calpeptin, or the highly specific calpain inhibitor protein, calpastatin. Capns1 -/- cells failed to reseal following laser-induced membrane disruption, demonstrating that their decreased survival after scraping resulted, at least in part, from failed membrane repair. Proteomic and immunologic analyses demonstrated that the known calpain substrates talin and vimentin were exposed at the cell surface and processed by calpain following cell scraping. Autoproteolytic activation of calpain at the scrape site was evident at the earliest time point analyzed and appeared to precede proteolysis of talin and vimentin. The results indicate that conventional calpains are required for calcium-facilitated survival after plasma membrane damage and may act by localized remodeling of the cortical cytoskeleton at the injury site.

Original languageEnglish (US)
Pages (from-to)2567-2575
Number of pages9
JournalJournal of Biological Chemistry
Volume282
Issue number4
DOIs
StatePublished - Jan 26 2007

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Calpain
Cell membranes
Repair
Cell Membrane
Calcium
Talin
Vimentin
Cells
Proteolysis
Membranes
Fibroblasts
Monolayers
Cricetulus
Cytoskeleton
Knockout Mice
Chemical activation
Proteomics
Ions
Ovary
Lasers

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Calpain is required for the rapid, calcium-dependent repair of wounded plasma membrane. / Mellgren, Ronald L.; Zhang, Wenli; Miyake, Katsuya; McNeil, Paul L.

In: Journal of Biological Chemistry, Vol. 282, No. 4, 26.01.2007, p. 2567-2575.

Research output: Contribution to journalArticle

Mellgren, Ronald L. ; Zhang, Wenli ; Miyake, Katsuya ; McNeil, Paul L. / Calpain is required for the rapid, calcium-dependent repair of wounded plasma membrane. In: Journal of Biological Chemistry. 2007 ; Vol. 282, No. 4. pp. 2567-2575.
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