Campylobacter fetus sap inversion occurs in the absence of RecA function

K. C. Ray, Z. C. Tu, R. Grogono-Thomas, D. G. Newell, Stuart A Thompson, M. J. Blaser

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Phase variation of Campylobacter fetus surface layer proteins (SLPs) occurs by inversion of a 6.2-kb DNA segment containing the unique sap promoter, permitting expression of a single SLP-encoding gene. Previous work has shown that the C. fetus sap inversion system is RecA dependent. When we challenged a pregnant ewe with a recA mutant of wild-type C. fetus (strain 97-211) that expressed the 97-kDa SLP, 15 of the 16 ovine-passaged isolates expressed the 97-kDa protein. However, one strain (97-209) expressed a 127-kDa SLP, suggesting that chromosomal rearrangement may have occurred to enable SLP switching. Lack of RecA function in strains 97-211 and 97-209 was confirmed by their sensitivity to the DNA-damaging agent methyl methane-sulfonate. Southern hybridization and PCR of these strains indicated that the aphA insertion into recA was stably present. However, Southern hybridizations demonstrated that in strain 97-209 inversion had occurred in the sap locus. PCR data confirmed inversion of the 6.2-kb DNA element and indicated that in these recA mutants the sap inversion frequency is reduced by 2 to 3 log10 units compared to that in the wild type. Thus, although the major sap inversion pathway in C. fetus is RecA dependent, alternative lower-frequency, RecA-independent inversion mechanisms exist.

Original languageEnglish (US)
Pages (from-to)5663-5667
Number of pages5
JournalInfection and Immunity
Volume68
Issue number10
DOIs
StatePublished - Jan 1 2000
Externally publishedYes

Fingerprint

Campylobacter fetus
DNA
Polymerase Chain Reaction
Methane
Sheep
S-layer proteins
Genes
Proteins

ASJC Scopus subject areas

  • Parasitology
  • Microbiology
  • Immunology
  • Infectious Diseases

Cite this

Campylobacter fetus sap inversion occurs in the absence of RecA function. / Ray, K. C.; Tu, Z. C.; Grogono-Thomas, R.; Newell, D. G.; Thompson, Stuart A; Blaser, M. J.

In: Infection and Immunity, Vol. 68, No. 10, 01.01.2000, p. 5663-5667.

Research output: Contribution to journalArticle

Ray, KC, Tu, ZC, Grogono-Thomas, R, Newell, DG, Thompson, SA & Blaser, MJ 2000, 'Campylobacter fetus sap inversion occurs in the absence of RecA function', Infection and Immunity, vol. 68, no. 10, pp. 5663-5667. https://doi.org/10.1128/IAI.68.10.5663-5667.2000
Ray, K. C. ; Tu, Z. C. ; Grogono-Thomas, R. ; Newell, D. G. ; Thompson, Stuart A ; Blaser, M. J. / Campylobacter fetus sap inversion occurs in the absence of RecA function. In: Infection and Immunity. 2000 ; Vol. 68, No. 10. pp. 5663-5667.
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