Candidate chemoreceptor subfamilies differentially expressed in the chemosensory organs of the mollusc Aplysia

Scott F. Cummins, Dirk Erpenbeck, Zhihua Zou, Charles Claudianos, Leonid L. Moroz, Gregg T. Nagle, Bernard M. Degnan

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Background: Marine molluscs, as is the case with most aquatic animals, rely heavily on olfactory cues for survival. In the mollusc Aplysia californica, mate-attraction is mediated by a blend of water-borne protein pheromones that are detected by sensory structures called rhinophores. The expression of G protein and phospholipase C signaling molecules in this organ is consistent with chemosensory detection being via a G-protein-coupled signaling mechanism. Results: Here we show that novel multi-transmembrane proteins with similarity to rhodopsin G-protein coupled receptors are expressed in sensory epithelia microdissected from the Aplysia rhinophore. Analysis of the A. californica genome reveals that these are part of larger multigene families that possess features found in metazoan chemosensory receptor families (that is, these families chiefly consist of single exon genes that are clustered in the genome). Phylogenetic analyses show that the novel Aplysia G-protein coupled receptor-like proteins represent three distinct monophyletic subfamilies. Representatives of each subfamily are restricted to or differentially expressed in the rhinophore and oral tentacles, suggesting that they encode functional chemoreceptors and that these olfactory organs sense different chemicals. Those expressed in rhinophores may sense water-borne pheromones. Secondary signaling component proteins Gαq, Gαi, and Gαo are also expressed in the rhinophore sensory epithelium. Conclusion: The novel rhodopsin G-protein coupled receptor-like gene subfamilies identified here do not have closely related identifiable orthologs in other metazoans, suggesting that they arose by a lineage-specific expansion as has been observed in chemosensory receptor families in other bilaterians. These candidate chemosensory receptors are expressed and often restricted to rhinophores and oral tentacles, lending support to the notion that water-borne chemical detection in Aplysia involves species- or lineage-specific families of chemosensory receptors.

Original languageEnglish (US)
Article number28
JournalBMC Biology
Volume7
DOIs
StatePublished - Jun 4 2009

Fingerprint

Molluscs
Aplysia
chemoreceptors
Mollusca
mollusc
molluscs
G-Protein-Coupled Receptors
Genes
receptors
rhodopsin
protein
Rhodopsin
Pheromones
G-proteins
GTP-Binding Proteins
pheromones
Water
mouth
epithelium
Epithelium

ASJC Scopus subject areas

  • Physiology
  • Biotechnology
  • Structural Biology
  • Developmental Biology
  • Plant Science
  • Ecology, Evolution, Behavior and Systematics
  • Cell Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Candidate chemoreceptor subfamilies differentially expressed in the chemosensory organs of the mollusc Aplysia. / Cummins, Scott F.; Erpenbeck, Dirk; Zou, Zhihua; Claudianos, Charles; Moroz, Leonid L.; Nagle, Gregg T.; Degnan, Bernard M.

In: BMC Biology, Vol. 7, 28, 04.06.2009.

Research output: Contribution to journalArticle

Cummins, Scott F. ; Erpenbeck, Dirk ; Zou, Zhihua ; Claudianos, Charles ; Moroz, Leonid L. ; Nagle, Gregg T. ; Degnan, Bernard M. / Candidate chemoreceptor subfamilies differentially expressed in the chemosensory organs of the mollusc Aplysia. In: BMC Biology. 2009 ; Vol. 7.
@article{cc43151805394d51a671ec6b970a14ae,
title = "Candidate chemoreceptor subfamilies differentially expressed in the chemosensory organs of the mollusc Aplysia",
abstract = "Background: Marine molluscs, as is the case with most aquatic animals, rely heavily on olfactory cues for survival. In the mollusc Aplysia californica, mate-attraction is mediated by a blend of water-borne protein pheromones that are detected by sensory structures called rhinophores. The expression of G protein and phospholipase C signaling molecules in this organ is consistent with chemosensory detection being via a G-protein-coupled signaling mechanism. Results: Here we show that novel multi-transmembrane proteins with similarity to rhodopsin G-protein coupled receptors are expressed in sensory epithelia microdissected from the Aplysia rhinophore. Analysis of the A. californica genome reveals that these are part of larger multigene families that possess features found in metazoan chemosensory receptor families (that is, these families chiefly consist of single exon genes that are clustered in the genome). Phylogenetic analyses show that the novel Aplysia G-protein coupled receptor-like proteins represent three distinct monophyletic subfamilies. Representatives of each subfamily are restricted to or differentially expressed in the rhinophore and oral tentacles, suggesting that they encode functional chemoreceptors and that these olfactory organs sense different chemicals. Those expressed in rhinophores may sense water-borne pheromones. Secondary signaling component proteins Gαq, Gαi, and Gαo are also expressed in the rhinophore sensory epithelium. Conclusion: The novel rhodopsin G-protein coupled receptor-like gene subfamilies identified here do not have closely related identifiable orthologs in other metazoans, suggesting that they arose by a lineage-specific expansion as has been observed in chemosensory receptor families in other bilaterians. These candidate chemosensory receptors are expressed and often restricted to rhinophores and oral tentacles, lending support to the notion that water-borne chemical detection in Aplysia involves species- or lineage-specific families of chemosensory receptors.",
author = "Cummins, {Scott F.} and Dirk Erpenbeck and Zhihua Zou and Charles Claudianos and Moroz, {Leonid L.} and Nagle, {Gregg T.} and Degnan, {Bernard M.}",
year = "2009",
month = "6",
day = "4",
doi = "10.1186/1741-7007-7-28",
language = "English (US)",
volume = "7",
journal = "BMC Biology",
issn = "1741-7007",
publisher = "BioMed Central",

}

TY - JOUR

T1 - Candidate chemoreceptor subfamilies differentially expressed in the chemosensory organs of the mollusc Aplysia

AU - Cummins, Scott F.

AU - Erpenbeck, Dirk

AU - Zou, Zhihua

AU - Claudianos, Charles

AU - Moroz, Leonid L.

AU - Nagle, Gregg T.

AU - Degnan, Bernard M.

PY - 2009/6/4

Y1 - 2009/6/4

N2 - Background: Marine molluscs, as is the case with most aquatic animals, rely heavily on olfactory cues for survival. In the mollusc Aplysia californica, mate-attraction is mediated by a blend of water-borne protein pheromones that are detected by sensory structures called rhinophores. The expression of G protein and phospholipase C signaling molecules in this organ is consistent with chemosensory detection being via a G-protein-coupled signaling mechanism. Results: Here we show that novel multi-transmembrane proteins with similarity to rhodopsin G-protein coupled receptors are expressed in sensory epithelia microdissected from the Aplysia rhinophore. Analysis of the A. californica genome reveals that these are part of larger multigene families that possess features found in metazoan chemosensory receptor families (that is, these families chiefly consist of single exon genes that are clustered in the genome). Phylogenetic analyses show that the novel Aplysia G-protein coupled receptor-like proteins represent three distinct monophyletic subfamilies. Representatives of each subfamily are restricted to or differentially expressed in the rhinophore and oral tentacles, suggesting that they encode functional chemoreceptors and that these olfactory organs sense different chemicals. Those expressed in rhinophores may sense water-borne pheromones. Secondary signaling component proteins Gαq, Gαi, and Gαo are also expressed in the rhinophore sensory epithelium. Conclusion: The novel rhodopsin G-protein coupled receptor-like gene subfamilies identified here do not have closely related identifiable orthologs in other metazoans, suggesting that they arose by a lineage-specific expansion as has been observed in chemosensory receptor families in other bilaterians. These candidate chemosensory receptors are expressed and often restricted to rhinophores and oral tentacles, lending support to the notion that water-borne chemical detection in Aplysia involves species- or lineage-specific families of chemosensory receptors.

AB - Background: Marine molluscs, as is the case with most aquatic animals, rely heavily on olfactory cues for survival. In the mollusc Aplysia californica, mate-attraction is mediated by a blend of water-borne protein pheromones that are detected by sensory structures called rhinophores. The expression of G protein and phospholipase C signaling molecules in this organ is consistent with chemosensory detection being via a G-protein-coupled signaling mechanism. Results: Here we show that novel multi-transmembrane proteins with similarity to rhodopsin G-protein coupled receptors are expressed in sensory epithelia microdissected from the Aplysia rhinophore. Analysis of the A. californica genome reveals that these are part of larger multigene families that possess features found in metazoan chemosensory receptor families (that is, these families chiefly consist of single exon genes that are clustered in the genome). Phylogenetic analyses show that the novel Aplysia G-protein coupled receptor-like proteins represent three distinct monophyletic subfamilies. Representatives of each subfamily are restricted to or differentially expressed in the rhinophore and oral tentacles, suggesting that they encode functional chemoreceptors and that these olfactory organs sense different chemicals. Those expressed in rhinophores may sense water-borne pheromones. Secondary signaling component proteins Gαq, Gαi, and Gαo are also expressed in the rhinophore sensory epithelium. Conclusion: The novel rhodopsin G-protein coupled receptor-like gene subfamilies identified here do not have closely related identifiable orthologs in other metazoans, suggesting that they arose by a lineage-specific expansion as has been observed in chemosensory receptor families in other bilaterians. These candidate chemosensory receptors are expressed and often restricted to rhinophores and oral tentacles, lending support to the notion that water-borne chemical detection in Aplysia involves species- or lineage-specific families of chemosensory receptors.

UR - http://www.scopus.com/inward/record.url?scp=67649697381&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=67649697381&partnerID=8YFLogxK

U2 - 10.1186/1741-7007-7-28

DO - 10.1186/1741-7007-7-28

M3 - Article

C2 - 19493360

AN - SCOPUS:67649697381

VL - 7

JO - BMC Biology

JF - BMC Biology

SN - 1741-7007

M1 - 28

ER -