TY - JOUR
T1 - Canine herpesvirus ORF2 is a membrane protein modified by N-linked glycosylation
AU - Nishikawa, Yoshifumi
AU - Kimura, Michiko
AU - Xuan, Xuenan
AU - Makala, Levi
AU - Nagasawa, Hideyuki
AU - Mikami, Takeshi
AU - Otsuka, Haruki
PY - 2002/8/3
Y1 - 2002/8/3
N2 - Canine herpesvirus (CHV) ORF2, located downstream of the glycoprotein C (gC) gene, has homologues with some of the alphaherpesviruses. To characterize CHV OFR2, a recombinant CHV carrying a LacZ gene in the ORF2 locus, and recombinant vaccinia virus expressing ORF2 protein were constructed. Northern blot analysis revealed ORF2 and a γ2 class late gene, and its protein product was detectable in CHV-infected cells reacted with ORF2 protein antiserum. Tunicamycin and N-glycosidase F treatment revealed that the ORF2 protein was modified by N-linked glycosylation. Fractionation and immune fluorescence analyses of the CHV-infected cells showed the ORF2 as a membrane protein transportable to the surface of infected cells. In vitro, the ORF2 protein did not affect viral replication and cell-to-cell viral spreading. Present findings represent the first evidence pointing to the CHV ORF2 as a membrane protein modified by an N-linked glycosylation.
AB - Canine herpesvirus (CHV) ORF2, located downstream of the glycoprotein C (gC) gene, has homologues with some of the alphaherpesviruses. To characterize CHV OFR2, a recombinant CHV carrying a LacZ gene in the ORF2 locus, and recombinant vaccinia virus expressing ORF2 protein were constructed. Northern blot analysis revealed ORF2 and a γ2 class late gene, and its protein product was detectable in CHV-infected cells reacted with ORF2 protein antiserum. Tunicamycin and N-glycosidase F treatment revealed that the ORF2 protein was modified by N-linked glycosylation. Fractionation and immune fluorescence analyses of the CHV-infected cells showed the ORF2 as a membrane protein transportable to the surface of infected cells. In vitro, the ORF2 protein did not affect viral replication and cell-to-cell viral spreading. Present findings represent the first evidence pointing to the CHV ORF2 as a membrane protein modified by an N-linked glycosylation.
KW - Canine herpesvirus
KW - N-linked glycosylation
KW - ORF2
UR - http://www.scopus.com/inward/record.url?scp=0036066403&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0036066403&partnerID=8YFLogxK
U2 - 10.1016/S0168-1702(01)00424-5
DO - 10.1016/S0168-1702(01)00424-5
M3 - Article
C2 - 12135784
AN - SCOPUS:0036066403
VL - 87
SP - 1
EP - 9
JO - Virus Research
JF - Virus Research
SN - 0168-1702
IS - 1
ER -