TY - JOUR
T1 - Canine herpesvirus ORF2 is a membrane protein modified by N-linked glycosylation
AU - Nishikawa, Yoshifumi
AU - Kimura, Michiko
AU - Xuan, Xuenan
AU - Makala, Levi
AU - Nagasawa, Hideyuki
AU - Mikami, Takeshi
AU - Otsuka, Haruki
N1 - Funding Information:
This work was supported by the grants from the Ministry of Education, Culture, Sports, Science and Technology of Japan. We appreciate the helpful inputs of Dr Florencia G. Claveria of the Biology Department, De La Salle University-Manila, Philipines during her sabbatical stay at the National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine. The Haruki Otsuka is supported by Research Fellowships of the Japan Society for the Promotion of Science for Young Scientists.
PY - 2002
Y1 - 2002
N2 - Canine herpesvirus (CHV) ORF2, located downstream of the glycoprotein C (gC) gene, has homologues with some of the alphaherpesviruses. To characterize CHV OFR2, a recombinant CHV carrying a LacZ gene in the ORF2 locus, and recombinant vaccinia virus expressing ORF2 protein were constructed. Northern blot analysis revealed ORF2 and a γ2 class late gene, and its protein product was detectable in CHV-infected cells reacted with ORF2 protein antiserum. Tunicamycin and N-glycosidase F treatment revealed that the ORF2 protein was modified by N-linked glycosylation. Fractionation and immune fluorescence analyses of the CHV-infected cells showed the ORF2 as a membrane protein transportable to the surface of infected cells. In vitro, the ORF2 protein did not affect viral replication and cell-to-cell viral spreading. Present findings represent the first evidence pointing to the CHV ORF2 as a membrane protein modified by an N-linked glycosylation.
AB - Canine herpesvirus (CHV) ORF2, located downstream of the glycoprotein C (gC) gene, has homologues with some of the alphaherpesviruses. To characterize CHV OFR2, a recombinant CHV carrying a LacZ gene in the ORF2 locus, and recombinant vaccinia virus expressing ORF2 protein were constructed. Northern blot analysis revealed ORF2 and a γ2 class late gene, and its protein product was detectable in CHV-infected cells reacted with ORF2 protein antiserum. Tunicamycin and N-glycosidase F treatment revealed that the ORF2 protein was modified by N-linked glycosylation. Fractionation and immune fluorescence analyses of the CHV-infected cells showed the ORF2 as a membrane protein transportable to the surface of infected cells. In vitro, the ORF2 protein did not affect viral replication and cell-to-cell viral spreading. Present findings represent the first evidence pointing to the CHV ORF2 as a membrane protein modified by an N-linked glycosylation.
KW - Canine herpesvirus
KW - N-linked glycosylation
KW - ORF2
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U2 - 10.1016/S0168-1702(01)00424-5
DO - 10.1016/S0168-1702(01)00424-5
M3 - Article
C2 - 12135784
AN - SCOPUS:0036066403
VL - 87
SP - 1
EP - 9
JO - Virus Research
JF - Virus Research
SN - 0168-1702
IS - 1
ER -