Canine herpesvirus ORF2 is a membrane protein modified by N-linked glycosylation

Yoshifumi Nishikawa, Michiko Kimura, Xuenan Xuan, Levi H Makala, Hideyuki Nagasawa, Takeshi Mikami, Haruki Otsuka

Research output: Contribution to journalArticle

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Abstract

Canine herpesvirus (CHV) ORF2, located downstream of the glycoprotein C (gC) gene, has homologues with some of the alphaherpesviruses. To characterize CHV OFR2, a recombinant CHV carrying a LacZ gene in the ORF2 locus, and recombinant vaccinia virus expressing ORF2 protein were constructed. Northern blot analysis revealed ORF2 and a γ2 class late gene, and its protein product was detectable in CHV-infected cells reacted with ORF2 protein antiserum. Tunicamycin and N-glycosidase F treatment revealed that the ORF2 protein was modified by N-linked glycosylation. Fractionation and immune fluorescence analyses of the CHV-infected cells showed the ORF2 as a membrane protein transportable to the surface of infected cells. In vitro, the ORF2 protein did not affect viral replication and cell-to-cell viral spreading. Present findings represent the first evidence pointing to the CHV ORF2 as a membrane protein modified by an N-linked glycosylation.

Original languageEnglish (US)
Pages (from-to)1-9
Number of pages9
JournalVirus Research
Volume87
Issue number1
DOIs
StatePublished - Aug 3 2002

Fingerprint

Herpesviridae
Glycosylation
Canidae
Membrane Proteins
Proteins
Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase
Tunicamycin
Lac Operon
Vaccinia virus
Northern Blotting
Immune Sera
Fluorescence
Genes

Keywords

  • Canine herpesvirus
  • N-linked glycosylation
  • ORF2

ASJC Scopus subject areas

  • Cancer Research
  • Virology
  • Infectious Diseases

Cite this

Nishikawa, Y., Kimura, M., Xuan, X., Makala, L. H., Nagasawa, H., Mikami, T., & Otsuka, H. (2002). Canine herpesvirus ORF2 is a membrane protein modified by N-linked glycosylation. Virus Research, 87(1), 1-9. https://doi.org/10.1016/S0168-1702(01)00424-5

Canine herpesvirus ORF2 is a membrane protein modified by N-linked glycosylation. / Nishikawa, Yoshifumi; Kimura, Michiko; Xuan, Xuenan; Makala, Levi H; Nagasawa, Hideyuki; Mikami, Takeshi; Otsuka, Haruki.

In: Virus Research, Vol. 87, No. 1, 03.08.2002, p. 1-9.

Research output: Contribution to journalArticle

Nishikawa, Y, Kimura, M, Xuan, X, Makala, LH, Nagasawa, H, Mikami, T & Otsuka, H 2002, 'Canine herpesvirus ORF2 is a membrane protein modified by N-linked glycosylation', Virus Research, vol. 87, no. 1, pp. 1-9. https://doi.org/10.1016/S0168-1702(01)00424-5
Nishikawa Y, Kimura M, Xuan X, Makala LH, Nagasawa H, Mikami T et al. Canine herpesvirus ORF2 is a membrane protein modified by N-linked glycosylation. Virus Research. 2002 Aug 3;87(1):1-9. https://doi.org/10.1016/S0168-1702(01)00424-5
Nishikawa, Yoshifumi ; Kimura, Michiko ; Xuan, Xuenan ; Makala, Levi H ; Nagasawa, Hideyuki ; Mikami, Takeshi ; Otsuka, Haruki. / Canine herpesvirus ORF2 is a membrane protein modified by N-linked glycosylation. In: Virus Research. 2002 ; Vol. 87, No. 1. pp. 1-9.
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