Abstract
Canine herpesvirus (CHV) ORF2, located downstream of the glycoprotein C (gC) gene, has homologues with some of the alphaherpesviruses. To characterize CHV OFR2, a recombinant CHV carrying a LacZ gene in the ORF2 locus, and recombinant vaccinia virus expressing ORF2 protein were constructed. Northern blot analysis revealed ORF2 and a γ2 class late gene, and its protein product was detectable in CHV-infected cells reacted with ORF2 protein antiserum. Tunicamycin and N-glycosidase F treatment revealed that the ORF2 protein was modified by N-linked glycosylation. Fractionation and immune fluorescence analyses of the CHV-infected cells showed the ORF2 as a membrane protein transportable to the surface of infected cells. In vitro, the ORF2 protein did not affect viral replication and cell-to-cell viral spreading. Present findings represent the first evidence pointing to the CHV ORF2 as a membrane protein modified by an N-linked glycosylation.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1-9 |
| Number of pages | 9 |
| Journal | Virus Research |
| Volume | 87 |
| Issue number | 1 |
| DOIs | |
| State | Published - Aug 3 2002 |
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Keywords
- Canine herpesvirus
- N-linked glycosylation
- ORF2
ASJC Scopus subject areas
- Cancer Research
- Virology
- Infectious Diseases
Cite this
Canine herpesvirus ORF2 is a membrane protein modified by N-linked glycosylation. / Nishikawa, Yoshifumi; Kimura, Michiko; Xuan, Xuenan; Makala, Levi H; Nagasawa, Hideyuki; Mikami, Takeshi; Otsuka, Haruki.
In: Virus Research, Vol. 87, No. 1, 03.08.2002, p. 1-9.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Canine herpesvirus ORF2 is a membrane protein modified by N-linked glycosylation
AU - Nishikawa, Yoshifumi
AU - Kimura, Michiko
AU - Xuan, Xuenan
AU - Makala, Levi H
AU - Nagasawa, Hideyuki
AU - Mikami, Takeshi
AU - Otsuka, Haruki
PY - 2002/8/3
Y1 - 2002/8/3
N2 - Canine herpesvirus (CHV) ORF2, located downstream of the glycoprotein C (gC) gene, has homologues with some of the alphaherpesviruses. To characterize CHV OFR2, a recombinant CHV carrying a LacZ gene in the ORF2 locus, and recombinant vaccinia virus expressing ORF2 protein were constructed. Northern blot analysis revealed ORF2 and a γ2 class late gene, and its protein product was detectable in CHV-infected cells reacted with ORF2 protein antiserum. Tunicamycin and N-glycosidase F treatment revealed that the ORF2 protein was modified by N-linked glycosylation. Fractionation and immune fluorescence analyses of the CHV-infected cells showed the ORF2 as a membrane protein transportable to the surface of infected cells. In vitro, the ORF2 protein did not affect viral replication and cell-to-cell viral spreading. Present findings represent the first evidence pointing to the CHV ORF2 as a membrane protein modified by an N-linked glycosylation.
AB - Canine herpesvirus (CHV) ORF2, located downstream of the glycoprotein C (gC) gene, has homologues with some of the alphaherpesviruses. To characterize CHV OFR2, a recombinant CHV carrying a LacZ gene in the ORF2 locus, and recombinant vaccinia virus expressing ORF2 protein were constructed. Northern blot analysis revealed ORF2 and a γ2 class late gene, and its protein product was detectable in CHV-infected cells reacted with ORF2 protein antiserum. Tunicamycin and N-glycosidase F treatment revealed that the ORF2 protein was modified by N-linked glycosylation. Fractionation and immune fluorescence analyses of the CHV-infected cells showed the ORF2 as a membrane protein transportable to the surface of infected cells. In vitro, the ORF2 protein did not affect viral replication and cell-to-cell viral spreading. Present findings represent the first evidence pointing to the CHV ORF2 as a membrane protein modified by an N-linked glycosylation.
KW - Canine herpesvirus
KW - N-linked glycosylation
KW - ORF2
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UR - http://www.scopus.com/inward/citedby.url?scp=0036066403&partnerID=8YFLogxK
U2 - 10.1016/S0168-1702(01)00424-5
DO - 10.1016/S0168-1702(01)00424-5
M3 - Article
C2 - 12135784
AN - SCOPUS:0036066403
VL - 87
SP - 1
EP - 9
JO - Virus Research
JF - Virus Research
SN - 0168-1702
IS - 1
ER -