Canine herpesvirus ORF2 is a membrane protein modified by N-linked glycosylation

Yoshifumi Nishikawa, Michiko Kimura, Xuenan Xuan, Levi Makala, Hideyuki Nagasawa, Takeshi Mikami, Haruki Otsuka

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1 Scopus citations

Abstract

Canine herpesvirus (CHV) ORF2, located downstream of the glycoprotein C (gC) gene, has homologues with some of the alphaherpesviruses. To characterize CHV OFR2, a recombinant CHV carrying a LacZ gene in the ORF2 locus, and recombinant vaccinia virus expressing ORF2 protein were constructed. Northern blot analysis revealed ORF2 and a γ2 class late gene, and its protein product was detectable in CHV-infected cells reacted with ORF2 protein antiserum. Tunicamycin and N-glycosidase F treatment revealed that the ORF2 protein was modified by N-linked glycosylation. Fractionation and immune fluorescence analyses of the CHV-infected cells showed the ORF2 as a membrane protein transportable to the surface of infected cells. In vitro, the ORF2 protein did not affect viral replication and cell-to-cell viral spreading. Present findings represent the first evidence pointing to the CHV ORF2 as a membrane protein modified by an N-linked glycosylation.

Original languageEnglish (US)
Pages (from-to)1-9
Number of pages9
JournalVirus Research
Volume87
Issue number1
DOIs
StatePublished - Aug 3 2002

Keywords

  • Canine herpesvirus
  • N-linked glycosylation
  • ORF2

ASJC Scopus subject areas

  • Cancer Research
  • Virology
  • Infectious Diseases

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    Nishikawa, Y., Kimura, M., Xuan, X., Makala, L., Nagasawa, H., Mikami, T., & Otsuka, H. (2002). Canine herpesvirus ORF2 is a membrane protein modified by N-linked glycosylation. Virus Research, 87(1), 1-9. https://doi.org/10.1016/S0168-1702(01)00424-5