Ca2+ imaging as a tool to assess TRP channel function in murine distal nephrons

Mykola Mamenko, Oleg Zaika, Roger G. O'Neil, Oleh Pochynyuk

Research output: Chapter in Book/Report/Conference proceedingChapter

9 Scopus citations

Abstract

Transient receptor potential (TRP) channels are expressed in almost every segment of renal nephron from the glomerulus to the inner medullary collecting duct. Serving as a route for Ca2+ entry from the intratubular space into cells in response to external cues, TRP channels modulate water-electrolyte transport, thus determining functional properties of the renal tubule. In this chapter, we discuss technical aspects of using Ca2+ imaging to monitor activity of TRP channels in situ, namely, in the freshly isolated distal nephrons, with a special emphasis on the mechanosensitive TRPV4 channel and its role in tubular flow sensing.

Original languageEnglish (US)
Title of host publicationIon Channels
Subtitle of host publicationMethods and Protocols
EditorsNikita Gamper
Pages371-384
Number of pages14
DOIs
StatePublished - May 8 2013
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume998
ISSN (Print)1064-3745

    Fingerprint

Keywords

  • Collecting duct
  • Connecting tubule
  • Fura-2
  • Mechanosensitivity
  • Shear stress

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Mamenko, M., Zaika, O., O'Neil, R. G., & Pochynyuk, O. (2013). Ca2+ imaging as a tool to assess TRP channel function in murine distal nephrons. In N. Gamper (Ed.), Ion Channels: Methods and Protocols (pp. 371-384). (Methods in Molecular Biology; Vol. 998). https://doi.org/10.1007/978-1-62703-351-0_29