Ca2+ imaging as a tool to assess TRP channel function in murine distal nephrons

Mykola Mamenko, Oleg Zaika, Roger G. O'Neil, Oleh Pochynyuk

Research output: Chapter in Book/Report/Conference proceedingChapter

8 Citations (Scopus)

Abstract

Transient receptor potential (TRP) channels are expressed in almost every segment of renal nephron from the glomerulus to the inner medullary collecting duct. Serving as a route for Ca2+ entry from the intratubular space into cells in response to external cues, TRP channels modulate water-electrolyte transport, thus determining functional properties of the renal tubule. In this chapter, we discuss technical aspects of using Ca2+ imaging to monitor activity of TRP channels in situ, namely, in the freshly isolated distal nephrons, with a special emphasis on the mechanosensitive TRPV4 channel and its role in tubular flow sensing.

Original languageEnglish (US)
Title of host publicationIon Channels
Subtitle of host publicationMethods and Protocols
Pages371-384
Number of pages14
DOIs
StatePublished - May 8 2013
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume998
ISSN (Print)1064-3745

Fingerprint

Transient Receptor Potential Channels
Nephrons
Kidney
Electrolytes
Cues
Water

Keywords

  • Collecting duct
  • Connecting tubule
  • Fura-2
  • Mechanosensitivity
  • Shear stress

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Mamenko, M., Zaika, O., O'Neil, R. G., & Pochynyuk, O. (2013). Ca2+ imaging as a tool to assess TRP channel function in murine distal nephrons. In Ion Channels: Methods and Protocols (pp. 371-384). (Methods in Molecular Biology; Vol. 998). https://doi.org/10.1007/978-1-62703-351-0_29

Ca2+ imaging as a tool to assess TRP channel function in murine distal nephrons. / Mamenko, Mykola; Zaika, Oleg; O'Neil, Roger G.; Pochynyuk, Oleh.

Ion Channels: Methods and Protocols. 2013. p. 371-384 (Methods in Molecular Biology; Vol. 998).

Research output: Chapter in Book/Report/Conference proceedingChapter

Mamenko, M, Zaika, O, O'Neil, RG & Pochynyuk, O 2013, Ca2+ imaging as a tool to assess TRP channel function in murine distal nephrons. in Ion Channels: Methods and Protocols. Methods in Molecular Biology, vol. 998, pp. 371-384. https://doi.org/10.1007/978-1-62703-351-0_29
Mamenko M, Zaika O, O'Neil RG, Pochynyuk O. Ca2+ imaging as a tool to assess TRP channel function in murine distal nephrons. In Ion Channels: Methods and Protocols. 2013. p. 371-384. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-62703-351-0_29
Mamenko, Mykola ; Zaika, Oleg ; O'Neil, Roger G. ; Pochynyuk, Oleh. / Ca2+ imaging as a tool to assess TRP channel function in murine distal nephrons. Ion Channels: Methods and Protocols. 2013. pp. 371-384 (Methods in Molecular Biology).
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