Cell wounding activates phospholipase D in primary mouse keratinocytes

Senthil N. Arun, Ding Xie, Amber C. Howard, Quincy Zhong, Xiaofeng Zhong, Paul L McNeil, Wendy B Bollag

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Plasma membrane disruptions occur in mechanically active tissues such as the epidermis and can lead to cell death if the damage remains unrepaired. Repair occurs through fusion of vesicle patches to the damaged membrane region. The enzyme phospholipase D (PLD) is involved in membrane traffi ckiing; therefore, the role of PLD in membrane repair was investigated. Generation of membrane disruptions by lifting epidermal keratinocytes from the substratum induced PLD activation, whereas removal of cells from the substratum via trypsinization had no effect. Pretreatment with 1,25-dihydroxyvitamin D 3 , previously shown to increase PLD1 expression and activity, had no effect on, and a PLD2-selective (but not a PLD1-selective) inhibitor decreased, cell lifting-induced PLD activation, suggesting PLD2 as the isoform activated. PLD2 interacts functionally with the glycerol channel aquaporin-3 (AQP3) to produce phosphatidylglycerol (PG); however, wounding resulted in decreased PG production, suggesting a potential PG defi ciency in wounded cells. Cell lifting-induced PLD activation was transient, consistent with a possible role in membrane repair, and PLD inhibitors inhibited membrane resealing upon laser injury. In an in vivo full-thickness mouse skin wound model, PG accelerated wound healing. These results suggest that PLD and the PLD2/AQP3 signaling module may be involved in membrane repair and wound healing.

Original languageEnglish (US)
Pages (from-to)581-591
Number of pages11
JournalJournal of Lipid Research
Volume54
Issue number3
DOIs
StatePublished - Mar 1 2013

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Phospholipase D
Keratinocytes
Phosphatidylglycerols
Membranes
Aquaporin 3
Repair
Chemical activation
Wound Healing
Wounds and Injuries
Cell death
Cell membranes
Epidermis
Glycerol
Skin
Protein Isoforms
Lasers
Cell Death
Fusion reactions
Cell Membrane
Tissue

Keywords

  • 5-fl uoro-2-indolyl des-chlorohalopemide
  • Aquaporin-3
  • Phosphatidylglycerol
  • Skin
  • Wound healing

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Cell Biology

Cite this

Cell wounding activates phospholipase D in primary mouse keratinocytes. / Arun, Senthil N.; Xie, Ding; Howard, Amber C.; Zhong, Quincy; Zhong, Xiaofeng; McNeil, Paul L; Bollag, Wendy B.

In: Journal of Lipid Research, Vol. 54, No. 3, 01.03.2013, p. 581-591.

Research output: Contribution to journalArticle

Arun, Senthil N. ; Xie, Ding ; Howard, Amber C. ; Zhong, Quincy ; Zhong, Xiaofeng ; McNeil, Paul L ; Bollag, Wendy B. / Cell wounding activates phospholipase D in primary mouse keratinocytes. In: Journal of Lipid Research. 2013 ; Vol. 54, No. 3. pp. 581-591.
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