Centrally applied atrial natriuretic factor diminishes bile secretion in the rat

Liliana G. Bianciotti, Marcelo S. Vatta, Cristina Vescina, Valeria Trippodi, Maria Eugenia Sabbatini, Belisario E. Fernandez

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Little is known about the role of centrally applied peptides in the regulation of bile secretion. We previously reported that the intravenous injection of atrial natriuretic factor (ANF) reduces bile acid dependent flow without affecting portal venous pressure in the rat. In the present work, we studied the effects of centrally applied ANF on bile secretion and the possible pathways involved. Rats were cannulated in the brain lateral ventricle for the administration of 1, 10 and 100 ng/μl ANF. After 1 week, the common bile duct was cannulated and bile samples were collected every 15 min for 60 min after the administration of ANF. The excretion rate of various biliary components was assessed. Bile secretion experiments were also performed after bilateral truncal vagotomy or atropine administration to evaluate the participation of a vagal pathway. In addition, the role of the sympathetic system was addressed by combined administration of propranolol and phentolamine. Centrally applied ANF did not modify blood pressure but diminished bile flow and bile acid output. It also reduced sodium and potassium secretion but did not modify protein or phospholipid excretion. Neither bilateral truncal vagotomy nor atropine administration abolished ANF response. Furthermore, combined administration of adrenergic antagonists did not alter ANF inhibitory effect on bile flow. In conclusion, centrally applied ANF reduced bile acid dependent flow not through a vagal or adrenergic pathway in the rat, suggesting the involvement of a peptidergic pathway.

Original languageEnglish (US)
Pages (from-to)127-133
Number of pages7
JournalRegulatory Peptides
Volume102
Issue number2-3
DOIs
StatePublished - Dec 15 2001
Externally publishedYes

Fingerprint

Atrial Natriuretic Factor
Bile
Rats
Bile Acids and Salts
Truncal Vagotomy
Atropine
Portal Pressure
Adrenergic Antagonists
Phentolamine
Lateral Ventricles
Secretory Pathway
Blood pressure
Common Bile Duct
Propranolol
Intravenous Injections
Adrenergic Agents
Ducts
Brain
Phospholipids
Potassium

Keywords

  • Bile acids
  • Bile flow
  • Central regulation
  • Natriuretic peptides

ASJC Scopus subject areas

  • Physiology
  • Biochemistry
  • Endocrinology
  • Clinical Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

Bianciotti, L. G., Vatta, M. S., Vescina, C., Trippodi, V., Sabbatini, M. E., & Fernandez, B. E. (2001). Centrally applied atrial natriuretic factor diminishes bile secretion in the rat. Regulatory Peptides, 102(2-3), 127-133. https://doi.org/10.1016/S0167-0115(01)00310-X

Centrally applied atrial natriuretic factor diminishes bile secretion in the rat. / Bianciotti, Liliana G.; Vatta, Marcelo S.; Vescina, Cristina; Trippodi, Valeria; Sabbatini, Maria Eugenia; Fernandez, Belisario E.

In: Regulatory Peptides, Vol. 102, No. 2-3, 15.12.2001, p. 127-133.

Research output: Contribution to journalArticle

Bianciotti, LG, Vatta, MS, Vescina, C, Trippodi, V, Sabbatini, ME & Fernandez, BE 2001, 'Centrally applied atrial natriuretic factor diminishes bile secretion in the rat', Regulatory Peptides, vol. 102, no. 2-3, pp. 127-133. https://doi.org/10.1016/S0167-0115(01)00310-X
Bianciotti, Liliana G. ; Vatta, Marcelo S. ; Vescina, Cristina ; Trippodi, Valeria ; Sabbatini, Maria Eugenia ; Fernandez, Belisario E. / Centrally applied atrial natriuretic factor diminishes bile secretion in the rat. In: Regulatory Peptides. 2001 ; Vol. 102, No. 2-3. pp. 127-133.
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