Cerebral blood flow and brain oxygenation in rats breathing oxygen under pressure

Ivan T. Demchenko, Yuriy I. Luchakov, Alexander N. Moskvin, Diana Raisovna Gutsaeva, Barry W. Allen, Edward D. Thalmann, Claude A. Piantadosi

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

Hyperbaric oxygen (HBO2) increases oxygen tension (PO 2) in blood but reduces blood flow by means of O2-induced vasoconstriction. Here we report the first quantitative evaluation of these opposing effects on tissue PO2 in brain, using anesthetized rats exposed to HBO2 at 2 to 6 atmospheres absolute (ATA). We assessed the contribution of regional cerebral blood flow (rCBF) to brain PO2 as inspired PO2 (PiO2) exceeds 1 ATA. We measured rCBF and local PO2 simultaneously in striatum using collocated platinum electrodes. Cerebral blood flow was computed from H2 clearance curves in vivo and PO2 from electrodes calibrated in vitro, before and after insertion. Arterial PCO2 was controlled, and body temperature, blood pressure, and EEG were monitored. Scatter plots of rCBF versus PO 2 were nonlinear (R2=0.75) for rats breathing room air but nearly linear (R2=0.88-0.91) for O2 at 2 to 6 ATA. The contribution of rCBF to brain PO2 was estimated at constant inspired PO2, by increasing rCBF with acetazolamide (AZA) or decreasing it with N-nitro-L-arginine methyl ester (L-NAME). At basal rCBF (78 mL/100 g min), local PO2 increased 7- to 33-fold at 2 to 6 ATA, compared with room air. A doubling of rCBF increased striatal PO2 not quite two-fold in rats breathing room air but 13- to 64-fold in those breathing HBO2 at 2 to 6 ATA. These findings support our hypothesis that HBO2 increases PO2 in brain in direct proportion to rCBF.

Original languageEnglish (US)
Pages (from-to)1288-1300
Number of pages13
JournalJournal of Cerebral Blood Flow and Metabolism
Volume25
Issue number10
DOIs
StatePublished - Oct 1 2005

Fingerprint

Cerebrovascular Circulation
Regional Blood Flow
Respiration
Oxygen
Pressure
Atmosphere
Brain
Air
Electrodes
Corpus Striatum
Acetazolamide
NG-Nitroarginine Methyl Ester
Vasoconstriction
Platinum
Body Temperature
Electroencephalography

Keywords

  • Brain
  • Cerebral blood flow
  • Hyperbaric oxygenation
  • Tissue oxygen tension

ASJC Scopus subject areas

  • Neurology
  • Clinical Neurology
  • Cardiology and Cardiovascular Medicine

Cite this

Demchenko, I. T., Luchakov, Y. I., Moskvin, A. N., Gutsaeva, D. R., Allen, B. W., Thalmann, E. D., & Piantadosi, C. A. (2005). Cerebral blood flow and brain oxygenation in rats breathing oxygen under pressure. Journal of Cerebral Blood Flow and Metabolism, 25(10), 1288-1300. https://doi.org/10.1038/sj.jcbfm.9600110

Cerebral blood flow and brain oxygenation in rats breathing oxygen under pressure. / Demchenko, Ivan T.; Luchakov, Yuriy I.; Moskvin, Alexander N.; Gutsaeva, Diana Raisovna; Allen, Barry W.; Thalmann, Edward D.; Piantadosi, Claude A.

In: Journal of Cerebral Blood Flow and Metabolism, Vol. 25, No. 10, 01.10.2005, p. 1288-1300.

Research output: Contribution to journalArticle

Demchenko, IT, Luchakov, YI, Moskvin, AN, Gutsaeva, DR, Allen, BW, Thalmann, ED & Piantadosi, CA 2005, 'Cerebral blood flow and brain oxygenation in rats breathing oxygen under pressure', Journal of Cerebral Blood Flow and Metabolism, vol. 25, no. 10, pp. 1288-1300. https://doi.org/10.1038/sj.jcbfm.9600110
Demchenko, Ivan T. ; Luchakov, Yuriy I. ; Moskvin, Alexander N. ; Gutsaeva, Diana Raisovna ; Allen, Barry W. ; Thalmann, Edward D. ; Piantadosi, Claude A. / Cerebral blood flow and brain oxygenation in rats breathing oxygen under pressure. In: Journal of Cerebral Blood Flow and Metabolism. 2005 ; Vol. 25, No. 10. pp. 1288-1300.
@article{35a95c2b1b114a23a739ce9e18609a0f,
title = "Cerebral blood flow and brain oxygenation in rats breathing oxygen under pressure",
abstract = "Hyperbaric oxygen (HBO2) increases oxygen tension (PO 2) in blood but reduces blood flow by means of O2-induced vasoconstriction. Here we report the first quantitative evaluation of these opposing effects on tissue PO2 in brain, using anesthetized rats exposed to HBO2 at 2 to 6 atmospheres absolute (ATA). We assessed the contribution of regional cerebral blood flow (rCBF) to brain PO2 as inspired PO2 (PiO2) exceeds 1 ATA. We measured rCBF and local PO2 simultaneously in striatum using collocated platinum electrodes. Cerebral blood flow was computed from H2 clearance curves in vivo and PO2 from electrodes calibrated in vitro, before and after insertion. Arterial PCO2 was controlled, and body temperature, blood pressure, and EEG were monitored. Scatter plots of rCBF versus PO 2 were nonlinear (R2=0.75) for rats breathing room air but nearly linear (R2=0.88-0.91) for O2 at 2 to 6 ATA. The contribution of rCBF to brain PO2 was estimated at constant inspired PO2, by increasing rCBF with acetazolamide (AZA) or decreasing it with N-nitro-L-arginine methyl ester (L-NAME). At basal rCBF (78 mL/100 g min), local PO2 increased 7- to 33-fold at 2 to 6 ATA, compared with room air. A doubling of rCBF increased striatal PO2 not quite two-fold in rats breathing room air but 13- to 64-fold in those breathing HBO2 at 2 to 6 ATA. These findings support our hypothesis that HBO2 increases PO2 in brain in direct proportion to rCBF.",
keywords = "Brain, Cerebral blood flow, Hyperbaric oxygenation, Tissue oxygen tension",
author = "Demchenko, {Ivan T.} and Luchakov, {Yuriy I.} and Moskvin, {Alexander N.} and Gutsaeva, {Diana Raisovna} and Allen, {Barry W.} and Thalmann, {Edward D.} and Piantadosi, {Claude A.}",
year = "2005",
month = "10",
day = "1",
doi = "10.1038/sj.jcbfm.9600110",
language = "English (US)",
volume = "25",
pages = "1288--1300",
journal = "Journal of Cerebral Blood Flow and Metabolism",
issn = "0271-678X",
publisher = "Nature Publishing Group",
number = "10",

}

TY - JOUR

T1 - Cerebral blood flow and brain oxygenation in rats breathing oxygen under pressure

AU - Demchenko, Ivan T.

AU - Luchakov, Yuriy I.

AU - Moskvin, Alexander N.

AU - Gutsaeva, Diana Raisovna

AU - Allen, Barry W.

AU - Thalmann, Edward D.

AU - Piantadosi, Claude A.

PY - 2005/10/1

Y1 - 2005/10/1

N2 - Hyperbaric oxygen (HBO2) increases oxygen tension (PO 2) in blood but reduces blood flow by means of O2-induced vasoconstriction. Here we report the first quantitative evaluation of these opposing effects on tissue PO2 in brain, using anesthetized rats exposed to HBO2 at 2 to 6 atmospheres absolute (ATA). We assessed the contribution of regional cerebral blood flow (rCBF) to brain PO2 as inspired PO2 (PiO2) exceeds 1 ATA. We measured rCBF and local PO2 simultaneously in striatum using collocated platinum electrodes. Cerebral blood flow was computed from H2 clearance curves in vivo and PO2 from electrodes calibrated in vitro, before and after insertion. Arterial PCO2 was controlled, and body temperature, blood pressure, and EEG were monitored. Scatter plots of rCBF versus PO 2 were nonlinear (R2=0.75) for rats breathing room air but nearly linear (R2=0.88-0.91) for O2 at 2 to 6 ATA. The contribution of rCBF to brain PO2 was estimated at constant inspired PO2, by increasing rCBF with acetazolamide (AZA) or decreasing it with N-nitro-L-arginine methyl ester (L-NAME). At basal rCBF (78 mL/100 g min), local PO2 increased 7- to 33-fold at 2 to 6 ATA, compared with room air. A doubling of rCBF increased striatal PO2 not quite two-fold in rats breathing room air but 13- to 64-fold in those breathing HBO2 at 2 to 6 ATA. These findings support our hypothesis that HBO2 increases PO2 in brain in direct proportion to rCBF.

AB - Hyperbaric oxygen (HBO2) increases oxygen tension (PO 2) in blood but reduces blood flow by means of O2-induced vasoconstriction. Here we report the first quantitative evaluation of these opposing effects on tissue PO2 in brain, using anesthetized rats exposed to HBO2 at 2 to 6 atmospheres absolute (ATA). We assessed the contribution of regional cerebral blood flow (rCBF) to brain PO2 as inspired PO2 (PiO2) exceeds 1 ATA. We measured rCBF and local PO2 simultaneously in striatum using collocated platinum electrodes. Cerebral blood flow was computed from H2 clearance curves in vivo and PO2 from electrodes calibrated in vitro, before and after insertion. Arterial PCO2 was controlled, and body temperature, blood pressure, and EEG were monitored. Scatter plots of rCBF versus PO 2 were nonlinear (R2=0.75) for rats breathing room air but nearly linear (R2=0.88-0.91) for O2 at 2 to 6 ATA. The contribution of rCBF to brain PO2 was estimated at constant inspired PO2, by increasing rCBF with acetazolamide (AZA) or decreasing it with N-nitro-L-arginine methyl ester (L-NAME). At basal rCBF (78 mL/100 g min), local PO2 increased 7- to 33-fold at 2 to 6 ATA, compared with room air. A doubling of rCBF increased striatal PO2 not quite two-fold in rats breathing room air but 13- to 64-fold in those breathing HBO2 at 2 to 6 ATA. These findings support our hypothesis that HBO2 increases PO2 in brain in direct proportion to rCBF.

KW - Brain

KW - Cerebral blood flow

KW - Hyperbaric oxygenation

KW - Tissue oxygen tension

UR - http://www.scopus.com/inward/record.url?scp=25144439082&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=25144439082&partnerID=8YFLogxK

U2 - 10.1038/sj.jcbfm.9600110

DO - 10.1038/sj.jcbfm.9600110

M3 - Article

C2 - 15789033

AN - SCOPUS:25144439082

VL - 25

SP - 1288

EP - 1300

JO - Journal of Cerebral Blood Flow and Metabolism

JF - Journal of Cerebral Blood Flow and Metabolism

SN - 0271-678X

IS - 10

ER -