Characterization and expression of novel 60-kDa and 110-kDa EGFR isoforms in human placenta

Jill L. Reiter, Nita Jane Maihle

Research output: Contribution to journalArticle

28 Scopus citations

Abstract

The epidermal growth factor receptor (EGFR) and related family members (ERBB2, ERBB3, and ERBB4) previously have been shown to play pivotal roles in the development of female reproductive tissues, in blastocyst implantation, and in placental differentiation. We have cloned and sequenced several naturally occurring alternative transcripts of the human and mouse EGFR genes, which encode novel receptor isoforms containing varying portions of the extracellular ligand-binding domain, but lacking the transmembrane and cytoplasmic domain sequences. The human 1.8-kb and 3-kb alternative EGFR transcripts encode secreted 60-kDa and cell surfaceassociated 110-kDa EGFR isoforms, respectively. We have developed quantitative ribonuclease protection assays to study the expression of these alternative transcripts in human tissues. Similar to the full-length EGFR mRNAs, the highest expression level of these alternative transcripts occurs in placenta. We speculate that both of these EGFR isoforms may be important regulators of EGF-mediated cell growth and differentiation in human placenta.

Original languageEnglish (US)
Pages (from-to)39-47
Number of pages9
JournalAnnals of the New York Academy of Sciences
Volume995
DOIs
Publication statusPublished - Jan 1 2003
Externally publishedYes

    Fingerprint

Keywords

  • Alternative splicing
  • Cell fractionation
  • Epidermal growth factor receptor
  • Glycoprotein
  • Isoforms
  • Placenta
  • Posttranslational protein modification
  • Receptor tyrosine kinase

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • History and Philosophy of Science

Cite this