Characterization of Biophysical and Metabolic Properties of Cells Labeled with Superparamagnetic Iron Oxide Nanoparticles and Transfection Agent for Cellular MR Imaging

Ali Syed Arbab, Lindsey A. Bashaw, Bradley R. Miller, Elaine K. Jordan, Bobbi K. Lewis, Heather Kalish, Joseph A. Frank

Research output: Contribution to journalArticle

511 Citations (Scopus)

Abstract

PURPOSE: To evaluate the effect of using the ferumoxides-poly-L-lysine (PLL) complex for magnetic cell labeling on the long-term viability, function, metabolism, and iron utilization of mammalian cells. MATERIALS AND METHODS: PLL was incubated with ferumoxides for 60 minutes, incompletely coating the superparamagnetic iron oxide (SPIO) through electrostatic interactions. Cells were coincubated overnight with the ferumoxides-PLL complex, and iron uptake, cell viability, apoptosis indexes, and reactive oxygen species formation were evaluated. The disappearance or the life span of the detectable iron nanoparticles in cells was also evaluated. The iron concentrations in the media also were assessed at different time points. Data were expressed as the mean ± 1 SD, and one-way analysis of variance and the unpaired Student t test were used to test for significant differences. RESULTS: Intracytoplasmic nanoparticles were stained with Prussian blue when the ferumoxides-PLL complex had magnetically labeled the human mesenchymal stem and HeLa cells. The long-term viability, growth rate, and apoptotic indexes of the labeled cells were unaffected by the endosomal incorporation of SPIO, as compared with these characteristics of the nonlabeled cells. In nondividing human mesenchymal stem cells, endosomal iron nanoparticles could be detected after 7 weeks; however, in rapidly dividing cells, intracellular iron had disappeared by five to eight divisions. A nonsignificant transient increase in reactive oxygen species production was seen in the human mesenchymal stem and HeLa cell lines. Labeled human mesenchymal stem cells did not differentiate to other lineage. A significant increase in iron concentration was observed in both the human mesenchymal stem and HeLa cell media at day 7. CONCLUSION: Magnetic cellular labeling with the ferumoxides-PLL complex had no short- or long-term toxic effects on tumor or stem cells.

Original languageEnglish (US)
Pages (from-to)838-846
Number of pages9
JournalRadiology
Volume229
Issue number3
DOIs
StatePublished - Dec 1 2003
Externally publishedYes

Fingerprint

Nanoparticles
Transfection
Iron
Mesenchymal Stromal Cells
Lysine
HeLa Cells
Reactive Oxygen Species
Neoplastic Stem Cells
Poisons
ferric oxide
Static Electricity
Cell Survival
Analysis of Variance
ferumoxides
Apoptosis
Students
Cell Line
Growth

Keywords

  • Cell labeling
  • Contrast media, experimental studies
  • Experimental study
  • Iron
  • Magnetic resonance (MR), contrast media
  • Radiobiology, cell and tissue studies

ASJC Scopus subject areas

  • Medicine(all)
  • Radiology Nuclear Medicine and imaging

Cite this

Characterization of Biophysical and Metabolic Properties of Cells Labeled with Superparamagnetic Iron Oxide Nanoparticles and Transfection Agent for Cellular MR Imaging. / Arbab, Ali Syed; Bashaw, Lindsey A.; Miller, Bradley R.; Jordan, Elaine K.; Lewis, Bobbi K.; Kalish, Heather; Frank, Joseph A.

In: Radiology, Vol. 229, No. 3, 01.12.2003, p. 838-846.

Research output: Contribution to journalArticle

Arbab, Ali Syed ; Bashaw, Lindsey A. ; Miller, Bradley R. ; Jordan, Elaine K. ; Lewis, Bobbi K. ; Kalish, Heather ; Frank, Joseph A. / Characterization of Biophysical and Metabolic Properties of Cells Labeled with Superparamagnetic Iron Oxide Nanoparticles and Transfection Agent for Cellular MR Imaging. In: Radiology. 2003 ; Vol. 229, No. 3. pp. 838-846.
@article{49ecd3bb5de94f998559f15f9cbad9c5,
title = "Characterization of Biophysical and Metabolic Properties of Cells Labeled with Superparamagnetic Iron Oxide Nanoparticles and Transfection Agent for Cellular MR Imaging",
abstract = "PURPOSE: To evaluate the effect of using the ferumoxides-poly-L-lysine (PLL) complex for magnetic cell labeling on the long-term viability, function, metabolism, and iron utilization of mammalian cells. MATERIALS AND METHODS: PLL was incubated with ferumoxides for 60 minutes, incompletely coating the superparamagnetic iron oxide (SPIO) through electrostatic interactions. Cells were coincubated overnight with the ferumoxides-PLL complex, and iron uptake, cell viability, apoptosis indexes, and reactive oxygen species formation were evaluated. The disappearance or the life span of the detectable iron nanoparticles in cells was also evaluated. The iron concentrations in the media also were assessed at different time points. Data were expressed as the mean ± 1 SD, and one-way analysis of variance and the unpaired Student t test were used to test for significant differences. RESULTS: Intracytoplasmic nanoparticles were stained with Prussian blue when the ferumoxides-PLL complex had magnetically labeled the human mesenchymal stem and HeLa cells. The long-term viability, growth rate, and apoptotic indexes of the labeled cells were unaffected by the endosomal incorporation of SPIO, as compared with these characteristics of the nonlabeled cells. In nondividing human mesenchymal stem cells, endosomal iron nanoparticles could be detected after 7 weeks; however, in rapidly dividing cells, intracellular iron had disappeared by five to eight divisions. A nonsignificant transient increase in reactive oxygen species production was seen in the human mesenchymal stem and HeLa cell lines. Labeled human mesenchymal stem cells did not differentiate to other lineage. A significant increase in iron concentration was observed in both the human mesenchymal stem and HeLa cell media at day 7. CONCLUSION: Magnetic cellular labeling with the ferumoxides-PLL complex had no short- or long-term toxic effects on tumor or stem cells.",
keywords = "Cell labeling, Contrast media, experimental studies, Experimental study, Iron, Magnetic resonance (MR), contrast media, Radiobiology, cell and tissue studies",
author = "Arbab, {Ali Syed} and Bashaw, {Lindsey A.} and Miller, {Bradley R.} and Jordan, {Elaine K.} and Lewis, {Bobbi K.} and Heather Kalish and Frank, {Joseph A.}",
year = "2003",
month = "12",
day = "1",
doi = "10.1148/radiol.2293021215",
language = "English (US)",
volume = "229",
pages = "838--846",
journal = "Radiology",
issn = "0033-8419",
publisher = "Radiological Society of North America Inc.",
number = "3",

}

TY - JOUR

T1 - Characterization of Biophysical and Metabolic Properties of Cells Labeled with Superparamagnetic Iron Oxide Nanoparticles and Transfection Agent for Cellular MR Imaging

AU - Arbab, Ali Syed

AU - Bashaw, Lindsey A.

AU - Miller, Bradley R.

AU - Jordan, Elaine K.

AU - Lewis, Bobbi K.

AU - Kalish, Heather

AU - Frank, Joseph A.

PY - 2003/12/1

Y1 - 2003/12/1

N2 - PURPOSE: To evaluate the effect of using the ferumoxides-poly-L-lysine (PLL) complex for magnetic cell labeling on the long-term viability, function, metabolism, and iron utilization of mammalian cells. MATERIALS AND METHODS: PLL was incubated with ferumoxides for 60 minutes, incompletely coating the superparamagnetic iron oxide (SPIO) through electrostatic interactions. Cells were coincubated overnight with the ferumoxides-PLL complex, and iron uptake, cell viability, apoptosis indexes, and reactive oxygen species formation were evaluated. The disappearance or the life span of the detectable iron nanoparticles in cells was also evaluated. The iron concentrations in the media also were assessed at different time points. Data were expressed as the mean ± 1 SD, and one-way analysis of variance and the unpaired Student t test were used to test for significant differences. RESULTS: Intracytoplasmic nanoparticles were stained with Prussian blue when the ferumoxides-PLL complex had magnetically labeled the human mesenchymal stem and HeLa cells. The long-term viability, growth rate, and apoptotic indexes of the labeled cells were unaffected by the endosomal incorporation of SPIO, as compared with these characteristics of the nonlabeled cells. In nondividing human mesenchymal stem cells, endosomal iron nanoparticles could be detected after 7 weeks; however, in rapidly dividing cells, intracellular iron had disappeared by five to eight divisions. A nonsignificant transient increase in reactive oxygen species production was seen in the human mesenchymal stem and HeLa cell lines. Labeled human mesenchymal stem cells did not differentiate to other lineage. A significant increase in iron concentration was observed in both the human mesenchymal stem and HeLa cell media at day 7. CONCLUSION: Magnetic cellular labeling with the ferumoxides-PLL complex had no short- or long-term toxic effects on tumor or stem cells.

AB - PURPOSE: To evaluate the effect of using the ferumoxides-poly-L-lysine (PLL) complex for magnetic cell labeling on the long-term viability, function, metabolism, and iron utilization of mammalian cells. MATERIALS AND METHODS: PLL was incubated with ferumoxides for 60 minutes, incompletely coating the superparamagnetic iron oxide (SPIO) through electrostatic interactions. Cells were coincubated overnight with the ferumoxides-PLL complex, and iron uptake, cell viability, apoptosis indexes, and reactive oxygen species formation were evaluated. The disappearance or the life span of the detectable iron nanoparticles in cells was also evaluated. The iron concentrations in the media also were assessed at different time points. Data were expressed as the mean ± 1 SD, and one-way analysis of variance and the unpaired Student t test were used to test for significant differences. RESULTS: Intracytoplasmic nanoparticles were stained with Prussian blue when the ferumoxides-PLL complex had magnetically labeled the human mesenchymal stem and HeLa cells. The long-term viability, growth rate, and apoptotic indexes of the labeled cells were unaffected by the endosomal incorporation of SPIO, as compared with these characteristics of the nonlabeled cells. In nondividing human mesenchymal stem cells, endosomal iron nanoparticles could be detected after 7 weeks; however, in rapidly dividing cells, intracellular iron had disappeared by five to eight divisions. A nonsignificant transient increase in reactive oxygen species production was seen in the human mesenchymal stem and HeLa cell lines. Labeled human mesenchymal stem cells did not differentiate to other lineage. A significant increase in iron concentration was observed in both the human mesenchymal stem and HeLa cell media at day 7. CONCLUSION: Magnetic cellular labeling with the ferumoxides-PLL complex had no short- or long-term toxic effects on tumor or stem cells.

KW - Cell labeling

KW - Contrast media, experimental studies

KW - Experimental study

KW - Iron

KW - Magnetic resonance (MR), contrast media

KW - Radiobiology, cell and tissue studies

UR - http://www.scopus.com/inward/record.url?scp=0344633614&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0344633614&partnerID=8YFLogxK

U2 - 10.1148/radiol.2293021215

DO - 10.1148/radiol.2293021215

M3 - Article

VL - 229

SP - 838

EP - 846

JO - Radiology

JF - Radiology

SN - 0033-8419

IS - 3

ER -