Characterization of purinergic receptor 2 signaling in podocytes from diabetic kidneys

Oleg Palygin, Christine A. Klemens, Elena Isaeva, Vladislav Levchenko, Denisha R. Spires, Lashodya V. Dissanayake, Oksana Nikolaienko, Daria V. Ilatovskaya, Alexander Staruschenko

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

Growing evidence suggests that renal purinergic signaling undergoes significant remodeling during pathophysiological conditions such as diabetes. This study examined the renal P2 receptor profile and ATP-mediated calcium response from podocytes in glomeruli from kidneys with type 1 or type 2 diabetic kidney disease (DKD), using type 2 diabetic nephropathy (T2DN) rats and streptozotocin-injected Dahl salt-sensitive (type 1 diabetes) rats. A dramatic increase in the ATP-mediated intracellular calcium flux in podocytes was observed in both models. Pharmacological inhibition established that P2X4 and P2X7 are the major receptors contributing to the augmented ATP-mediated intracellular calcium signaling in diabetic podocytes. The transition in purinergic receptor composition from metabotropic to ionotropic may disrupt intracellular calcium homeostasis in podocytes resulting in their dysfunction and potentially further aggravating DKD progression.

Original languageEnglish (US)
Article number102528
JournaliScience
Volume24
Issue number6
DOIs
StatePublished - Jun 25 2021
Externally publishedYes

ASJC Scopus subject areas

  • General

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