Characterization of the DNA-melting function of the Rickettsia prowazekii RNA polymerase

Hanfei Ding, H. H. Winkler

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

In vitro specific transcription by the Rickettsia prowazekii RNA polymerase was investigated. The purified rickettsial RNA polymerase, in striking contrast to that of Escherichia coli, could specifically transcribe two R. prowazekii genes (ATP/ADP translocase and citrate synthase genes) and one E. coli gene (RNA-I) on negative supercoiled plasmids but not the same genes on linear plasmids. Following the specific binding of the rickettsial RNA polymerase to the translocase gene promoter on a linear plasmid, there was no detectable open complex formation. Both the E. coli and the R. prowazekii RNA polymerases worked well when poly(dA-dT)·poly(dA-dT) or poly(dI-dC)·poly(dI-dC) was used as template for generalized transcription. However, the rickettsial RNA polymerase, in contrast to the E. coli enzyme, had little activity on poly(dG-dC)·poly(dG-dC), a template with a larger number of hydrogen bonds. These data indicate that the rickettsial RNA polymerase is weak, at least relative to E. coli, in the function required for the opening of DNA duplex. It appears that this operation in R. prowazekii is aided by the negative supercoiling and the high 72% AT composition of the rickettsial genome.

Original languageEnglish (US)
Pages (from-to)3897-3902
Number of pages6
JournalJournal of Biological Chemistry
Volume268
Issue number6
StatePublished - Jan 1 1993
Externally publishedYes

Fingerprint

Rickettsia prowazekii
Nucleic Acid Denaturation
DNA-Directed RNA Polymerases
Melting
Escherichia coli
Genes
DNA
Plasmids
Transcription
Poly dA-dT
ATP Translocases Mitochondrial ADP
vpr Genes
Citrate (si)-Synthase
Hydrogen
Hydrogen bonds
Genome
Enzymes
Chemical analysis

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Characterization of the DNA-melting function of the Rickettsia prowazekii RNA polymerase. / Ding, Hanfei; Winkler, H. H.

In: Journal of Biological Chemistry, Vol. 268, No. 6, 01.01.1993, p. 3897-3902.

Research output: Contribution to journalArticle

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