Characterization of tumor-associated fucogangliosides from PC 12 pheochromocytoma cells

T. Ariga, K. Kobayashi, Y. Kuroda, Robert K Yu, M. Suzuki, H. Kitagawa, F. Inagaki, T. Miyatake

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

PC 12h pheochromocytoma cells were subcutaneously transplanted into rat. We found the transplanted tumors accumulated some fucoganglioside associated with PC 12 cells. These gangliosides were isolated and purified by DEAE-Sephadex A-25 and Iatrobeads column chromatographies. Their structures were determined by fast atom bombardment mass spectrometry, proton nuclear magnetic resonance spectrometry, permethylation study, and sequential degradation using various exoglycosidases and mild acid hydrolysis. Two tumor-associated fucogangliosides were found to possess the blood group B determinant as follows: G6: IV2Fucα,IV3Galα,II3NeuAc,GgOse4cer;G11:IV2Fucα,IV3Galα,II3 (NeuAc)2,GgOse4Cer. A ganglioside with the similar structure as ganglioside G6 was isolated from rat hepatoma cells (Holmes, E.H., and Hakomori, S-I. (1982) J. Biol. Chem. 257, 7698-7703). However, ganglioside G11 has not previously been reported in the literature. These fucogangliosides reacted with the monoclonal antibody prepared by immunizing mice with PC 12h cells. Other fucogangliosides were also found to accumulate in the transplanted tumor tissues. They were identified as fucosyl-G(M1) and fucosyl-G(Dlb). These fucogangiosides did not react with the monoclonal antibody against PC 12h cells.

Original languageEnglish (US)
Pages (from-to)14146-14153
Number of pages8
JournalJournal of Biological Chemistry
Volume262
Issue number29
StatePublished - Dec 1 1987
Externally publishedYes

Fingerprint

Gangliosides
Pheochromocytoma
Tumors
Rats
Neoplasms
Monoclonal Antibodies
Nuclear magnetic resonance
Column chromatography
Glycoside Hydrolases
Fast Atom Bombardment Mass Spectrometry
Blood Group Antigens
Spectrometry
Mass spectrometry
Hydrolysis
Tissue
Protons
Chromatography
Hepatocellular Carcinoma
Spectrum Analysis
Degradation

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Ariga, T., Kobayashi, K., Kuroda, Y., Yu, R. K., Suzuki, M., Kitagawa, H., ... Miyatake, T. (1987). Characterization of tumor-associated fucogangliosides from PC 12 pheochromocytoma cells. Journal of Biological Chemistry, 262(29), 14146-14153.

Characterization of tumor-associated fucogangliosides from PC 12 pheochromocytoma cells. / Ariga, T.; Kobayashi, K.; Kuroda, Y.; Yu, Robert K; Suzuki, M.; Kitagawa, H.; Inagaki, F.; Miyatake, T.

In: Journal of Biological Chemistry, Vol. 262, No. 29, 01.12.1987, p. 14146-14153.

Research output: Contribution to journalArticle

Ariga, T, Kobayashi, K, Kuroda, Y, Yu, RK, Suzuki, M, Kitagawa, H, Inagaki, F & Miyatake, T 1987, 'Characterization of tumor-associated fucogangliosides from PC 12 pheochromocytoma cells', Journal of Biological Chemistry, vol. 262, no. 29, pp. 14146-14153.
Ariga T, Kobayashi K, Kuroda Y, Yu RK, Suzuki M, Kitagawa H et al. Characterization of tumor-associated fucogangliosides from PC 12 pheochromocytoma cells. Journal of Biological Chemistry. 1987 Dec 1;262(29):14146-14153.
Ariga, T. ; Kobayashi, K. ; Kuroda, Y. ; Yu, Robert K ; Suzuki, M. ; Kitagawa, H. ; Inagaki, F. ; Miyatake, T. / Characterization of tumor-associated fucogangliosides from PC 12 pheochromocytoma cells. In: Journal of Biological Chemistry. 1987 ; Vol. 262, No. 29. pp. 14146-14153.
@article{cfaffae57c0541febf05885fda4660f2,
title = "Characterization of tumor-associated fucogangliosides from PC 12 pheochromocytoma cells",
abstract = "PC 12h pheochromocytoma cells were subcutaneously transplanted into rat. We found the transplanted tumors accumulated some fucoganglioside associated with PC 12 cells. These gangliosides were isolated and purified by DEAE-Sephadex A-25 and Iatrobeads column chromatographies. Their structures were determined by fast atom bombardment mass spectrometry, proton nuclear magnetic resonance spectrometry, permethylation study, and sequential degradation using various exoglycosidases and mild acid hydrolysis. Two tumor-associated fucogangliosides were found to possess the blood group B determinant as follows: G6: IV2Fucα,IV3Galα,II3NeuAc,GgOse4cer;G11:IV2Fucα,IV3Galα,II3 (NeuAc)2,GgOse4Cer. A ganglioside with the similar structure as ganglioside G6 was isolated from rat hepatoma cells (Holmes, E.H., and Hakomori, S-I. (1982) J. Biol. Chem. 257, 7698-7703). However, ganglioside G11 has not previously been reported in the literature. These fucogangliosides reacted with the monoclonal antibody prepared by immunizing mice with PC 12h cells. Other fucogangliosides were also found to accumulate in the transplanted tumor tissues. They were identified as fucosyl-G(M1) and fucosyl-G(Dlb). These fucogangiosides did not react with the monoclonal antibody against PC 12h cells.",
author = "T. Ariga and K. Kobayashi and Y. Kuroda and Yu, {Robert K} and M. Suzuki and H. Kitagawa and F. Inagaki and T. Miyatake",
year = "1987",
month = "12",
day = "1",
language = "English (US)",
volume = "262",
pages = "14146--14153",
journal = "Journal of Biological Chemistry",
issn = "0021-9258",
publisher = "American Society for Biochemistry and Molecular Biology Inc.",
number = "29",

}

TY - JOUR

T1 - Characterization of tumor-associated fucogangliosides from PC 12 pheochromocytoma cells

AU - Ariga, T.

AU - Kobayashi, K.

AU - Kuroda, Y.

AU - Yu, Robert K

AU - Suzuki, M.

AU - Kitagawa, H.

AU - Inagaki, F.

AU - Miyatake, T.

PY - 1987/12/1

Y1 - 1987/12/1

N2 - PC 12h pheochromocytoma cells were subcutaneously transplanted into rat. We found the transplanted tumors accumulated some fucoganglioside associated with PC 12 cells. These gangliosides were isolated and purified by DEAE-Sephadex A-25 and Iatrobeads column chromatographies. Their structures were determined by fast atom bombardment mass spectrometry, proton nuclear magnetic resonance spectrometry, permethylation study, and sequential degradation using various exoglycosidases and mild acid hydrolysis. Two tumor-associated fucogangliosides were found to possess the blood group B determinant as follows: G6: IV2Fucα,IV3Galα,II3NeuAc,GgOse4cer;G11:IV2Fucα,IV3Galα,II3 (NeuAc)2,GgOse4Cer. A ganglioside with the similar structure as ganglioside G6 was isolated from rat hepatoma cells (Holmes, E.H., and Hakomori, S-I. (1982) J. Biol. Chem. 257, 7698-7703). However, ganglioside G11 has not previously been reported in the literature. These fucogangliosides reacted with the monoclonal antibody prepared by immunizing mice with PC 12h cells. Other fucogangliosides were also found to accumulate in the transplanted tumor tissues. They were identified as fucosyl-G(M1) and fucosyl-G(Dlb). These fucogangiosides did not react with the monoclonal antibody against PC 12h cells.

AB - PC 12h pheochromocytoma cells were subcutaneously transplanted into rat. We found the transplanted tumors accumulated some fucoganglioside associated with PC 12 cells. These gangliosides were isolated and purified by DEAE-Sephadex A-25 and Iatrobeads column chromatographies. Their structures were determined by fast atom bombardment mass spectrometry, proton nuclear magnetic resonance spectrometry, permethylation study, and sequential degradation using various exoglycosidases and mild acid hydrolysis. Two tumor-associated fucogangliosides were found to possess the blood group B determinant as follows: G6: IV2Fucα,IV3Galα,II3NeuAc,GgOse4cer;G11:IV2Fucα,IV3Galα,II3 (NeuAc)2,GgOse4Cer. A ganglioside with the similar structure as ganglioside G6 was isolated from rat hepatoma cells (Holmes, E.H., and Hakomori, S-I. (1982) J. Biol. Chem. 257, 7698-7703). However, ganglioside G11 has not previously been reported in the literature. These fucogangliosides reacted with the monoclonal antibody prepared by immunizing mice with PC 12h cells. Other fucogangliosides were also found to accumulate in the transplanted tumor tissues. They were identified as fucosyl-G(M1) and fucosyl-G(Dlb). These fucogangiosides did not react with the monoclonal antibody against PC 12h cells.

UR - http://www.scopus.com/inward/record.url?scp=0023629564&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023629564&partnerID=8YFLogxK

M3 - Article

C2 - 3654655

AN - SCOPUS:0023629564

VL - 262

SP - 14146

EP - 14153

JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

SN - 0021-9258

IS - 29

ER -