Characterization of tumor-associated fucogangliosides from PC 12 pheochromocytoma cells

T. Ariga, K. Kobayashi, Y. Kuroda, Robert K Yu, M. Suzuki, H. Kitagawa, F. Inagaki, T. Miyatake

Research output: Contribution to journalArticlepeer-review

33 Scopus citations

Abstract

PC 12h pheochromocytoma cells were subcutaneously transplanted into rat. We found the transplanted tumors accumulated some fucoganglioside associated with PC 12 cells. These gangliosides were isolated and purified by DEAE-Sephadex A-25 and Iatrobeads column chromatographies. Their structures were determined by fast atom bombardment mass spectrometry, proton nuclear magnetic resonance spectrometry, permethylation study, and sequential degradation using various exoglycosidases and mild acid hydrolysis. Two tumor-associated fucogangliosides were found to possess the blood group B determinant as follows: G6: IV2Fucα,IV3Galα,II3NeuAc,GgOse4cer;G11:IV2Fucα,IV3Galα,II3 (NeuAc)2,GgOse4Cer. A ganglioside with the similar structure as ganglioside G6 was isolated from rat hepatoma cells (Holmes, E.H., and Hakomori, S-I. (1982) J. Biol. Chem. 257, 7698-7703). However, ganglioside G11 has not previously been reported in the literature. These fucogangliosides reacted with the monoclonal antibody prepared by immunizing mice with PC 12h cells. Other fucogangliosides were also found to accumulate in the transplanted tumor tissues. They were identified as fucosyl-G(M1) and fucosyl-G(Dlb). These fucogangiosides did not react with the monoclonal antibody against PC 12h cells.

Original languageEnglish (US)
Pages (from-to)14146-14153
Number of pages8
JournalJournal of Biological Chemistry
Volume262
Issue number29
StatePublished - Dec 1 1987
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Fingerprint Dive into the research topics of 'Characterization of tumor-associated fucogangliosides from PC 12 pheochromocytoma cells'. Together they form a unique fingerprint.

Cite this