Chemically defined and xeno-free cryopreservation of human-induced pluripotent stem cells

Juliette Seremak, Ali Eroglu

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Human-induced pluripotent stem cells (hiPSCs) can be derived from a variety of biopsy samples and have an unlimited capacity for self-renewal and differentiation into almost any cell type in the body. Therefore, hiPSCs offer unprecedented opportunities for patient-specific cell therapies, modeling of human diseases, biomarker discovery, and drug testing. However, clinical applications of hiPSCs require xeno-free and, ideally, chemically defined methods for their generation, expansion, and cryopreservation. In this chapter, we present a chemically defined and xeno-free slow freezing method for hiPSCs along with a chemically undefined protocol. Both approaches yield reasonable post-thaw viability and cell growth.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages569-579
Number of pages11
DOIs
StatePublished - 2021

Publication series

NameMethods in Molecular Biology
Volume2180
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Chemically defined
  • Clinical-grade cryopreservation
  • Cryopreservation
  • Human-induced pluripotent stem cells (iPSCs)
  • Xeno-free

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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  • Cite this

    Seremak, J., & Eroglu, A. (2021). Chemically defined and xeno-free cryopreservation of human-induced pluripotent stem cells. In Methods in Molecular Biology (pp. 569-579). (Methods in Molecular Biology; Vol. 2180). Humana Press Inc.. https://doi.org/10.1007/978-1-0716-0783-1_29