Chemically defined and xeno-free cryopreservation of human-induced pluripotent stem cells

Juliette Seremak, Ali Eroglu

Research output: Chapter in Book/Report/Conference proceedingChapter

2 Scopus citations


Human-induced pluripotent stem cells (hiPSCs) can be derived from a variety of biopsy samples and have an unlimited capacity for self-renewal and differentiation into almost any cell type in the body. Therefore, hiPSCs offer unprecedented opportunities for patient-specific cell therapies, modeling of human diseases, biomarker discovery, and drug testing. However, clinical applications of hiPSCs require xeno-free and, ideally, chemically defined methods for their generation, expansion, and cryopreservation. In this chapter, we present a chemically defined and xeno-free slow freezing method for hiPSCs along with a chemically undefined protocol. Both approaches yield reasonable post-thaw viability and cell growth.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Number of pages11
StatePublished - 2021

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029


  • Chemically defined
  • Clinical-grade cryopreservation
  • Cryopreservation
  • Human-induced pluripotent stem cells (iPSCs)
  • Xeno-free

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics


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