Chemically defined, clinical-grade cryopreservation of human adipose stem cells

Melany López, Ali Eroglu

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Adipose-derived stem cells (ASCs) reside in the stromal compartment of adipose tissue and can be easily harvested in large quantities through a clinically safe liposuction procedure. ASCs do not induce immunogenic reactions and rather exert immunosuppressive effects. Therefore, they can be used for both autologous and allogeneic transplantations. They hold great promise for cell-based therapies and tissue engineering. A prerequisite to the realization of this promise is the development of successful cryopreservation methods for ASCs. In this chapter, we describe a xeno-free- and chemically defined cryopreservation protocol, which can be used for various clinical applications of ASCs.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages555-567
Number of pages13
DOIs
StatePublished - 2021

Publication series

NameMethods in Molecular Biology
Volume2180
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Clinical-grade cryopreservation
  • Cryopreservation
  • Human adipose stem cells

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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