Chlorhexidine binding to mineralized versus demineralized dentin powder

Jongryul Kim, Toshikazu Uchiyama, Marcela Carrilho, Kelli A. Agee, Annalisa Mazzoni, Lorenzo Breschi, Ricardo M. Carvalho, Leo Tjäderhane, Stephen Warwick Looney, Courtney Wimmer, Arzu Tezvergil-Mutluay, Franklin Chi Meng Tay, David Henry Pashley

Research output: Contribution to journalArticle

85 Citations (Scopus)

Abstract

Objectives: The purposes of this work were to quantitate the affinity and binding capacity of chlorhexidine (CHX) digluconate to mineralized versus demineralized dentin powder and to determine how much debinding would result from rinsing with water, ethanol, hydroxyethylmethacrylate (HEMA) or 0.5 M NaCl in water. Methods: Dentin powder was made from coronal dentin of extracted human third molars. Standard amounts of dentin powder were tumbled with increasing concentrations of CHX (0-30 mM) for 30 min at 37 °C. After centrifuging the tubes, the supernatant was removed and the decrease in CHX concentration quantitated by UV-spectroscopy. CHX-treated dentin powder was resuspended in one of the four debinding solutions for 3 min. The amount of debound CHX in the solvents was also quantitated by UV-spectroscopy. Results: As the CHX concentration in the medium increased, the CHX binding to mineralized dentin powder also increased up to 6.8 μmol/g of dry dentin powder. Demineralized dentin powder took up significantly (p < 0.01) more CHX, reaching 30.1 μmol CHX/g of dry dentin powder. Debinding of CHX was in the order: HEMA < ethanol < 0.05 M NaCl < water. The highest CHX binding to demineralized dentin occurred at 30 mM (1.5 wt.%). Significance: As CHX is not debound by HEMA, it may remain bound to demineralized dentin during resin-dentin bonding. This may be responsible for the long-term efficacy of CHX as an MMP inhibitor in resin-dentin bonds.

Original languageEnglish (US)
Pages (from-to)771-778
Number of pages8
JournalDental Materials
Volume26
Issue number8
DOIs
StatePublished - Aug 1 2010

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Chlorhexidine
Dentin
Powders
Ultraviolet spectroscopy
Ethanol
Resins
Water
X ray powder diffraction
Spectrum Analysis
Matrix Metalloproteinase Inhibitors
Third Molar

Keywords

  • Binding
  • Chlorhexidine
  • Debinding
  • Dentin bonding

ASJC Scopus subject areas

  • Materials Science(all)
  • Dentistry(all)
  • Mechanics of Materials

Cite this

Kim, J., Uchiyama, T., Carrilho, M., Agee, K. A., Mazzoni, A., Breschi, L., ... Pashley, D. H. (2010). Chlorhexidine binding to mineralized versus demineralized dentin powder. Dental Materials, 26(8), 771-778. https://doi.org/10.1016/j.dental.2010.04.001

Chlorhexidine binding to mineralized versus demineralized dentin powder. / Kim, Jongryul; Uchiyama, Toshikazu; Carrilho, Marcela; Agee, Kelli A.; Mazzoni, Annalisa; Breschi, Lorenzo; Carvalho, Ricardo M.; Tjäderhane, Leo; Looney, Stephen Warwick; Wimmer, Courtney; Tezvergil-Mutluay, Arzu; Tay, Franklin Chi Meng; Pashley, David Henry.

In: Dental Materials, Vol. 26, No. 8, 01.08.2010, p. 771-778.

Research output: Contribution to journalArticle

Kim, J, Uchiyama, T, Carrilho, M, Agee, KA, Mazzoni, A, Breschi, L, Carvalho, RM, Tjäderhane, L, Looney, SW, Wimmer, C, Tezvergil-Mutluay, A, Tay, FCM & Pashley, DH 2010, 'Chlorhexidine binding to mineralized versus demineralized dentin powder', Dental Materials, vol. 26, no. 8, pp. 771-778. https://doi.org/10.1016/j.dental.2010.04.001
Kim J, Uchiyama T, Carrilho M, Agee KA, Mazzoni A, Breschi L et al. Chlorhexidine binding to mineralized versus demineralized dentin powder. Dental Materials. 2010 Aug 1;26(8):771-778. https://doi.org/10.1016/j.dental.2010.04.001
Kim, Jongryul ; Uchiyama, Toshikazu ; Carrilho, Marcela ; Agee, Kelli A. ; Mazzoni, Annalisa ; Breschi, Lorenzo ; Carvalho, Ricardo M. ; Tjäderhane, Leo ; Looney, Stephen Warwick ; Wimmer, Courtney ; Tezvergil-Mutluay, Arzu ; Tay, Franklin Chi Meng ; Pashley, David Henry. / Chlorhexidine binding to mineralized versus demineralized dentin powder. In: Dental Materials. 2010 ; Vol. 26, No. 8. pp. 771-778.
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AU - Mazzoni, Annalisa

AU - Breschi, Lorenzo

AU - Carvalho, Ricardo M.

AU - Tjäderhane, Leo

AU - Looney, Stephen Warwick

AU - Wimmer, Courtney

AU - Tezvergil-Mutluay, Arzu

AU - Tay, Franklin Chi Meng

AU - Pashley, David Henry

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N2 - Objectives: The purposes of this work were to quantitate the affinity and binding capacity of chlorhexidine (CHX) digluconate to mineralized versus demineralized dentin powder and to determine how much debinding would result from rinsing with water, ethanol, hydroxyethylmethacrylate (HEMA) or 0.5 M NaCl in water. Methods: Dentin powder was made from coronal dentin of extracted human third molars. Standard amounts of dentin powder were tumbled with increasing concentrations of CHX (0-30 mM) for 30 min at 37 °C. After centrifuging the tubes, the supernatant was removed and the decrease in CHX concentration quantitated by UV-spectroscopy. CHX-treated dentin powder was resuspended in one of the four debinding solutions for 3 min. The amount of debound CHX in the solvents was also quantitated by UV-spectroscopy. Results: As the CHX concentration in the medium increased, the CHX binding to mineralized dentin powder also increased up to 6.8 μmol/g of dry dentin powder. Demineralized dentin powder took up significantly (p < 0.01) more CHX, reaching 30.1 μmol CHX/g of dry dentin powder. Debinding of CHX was in the order: HEMA < ethanol < 0.05 M NaCl < water. The highest CHX binding to demineralized dentin occurred at 30 mM (1.5 wt.%). Significance: As CHX is not debound by HEMA, it may remain bound to demineralized dentin during resin-dentin bonding. This may be responsible for the long-term efficacy of CHX as an MMP inhibitor in resin-dentin bonds.

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KW - Chlorhexidine

KW - Debinding

KW - Dentin bonding

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