TY - JOUR
T1 - Chlorhexidine binding to mineralized versus demineralized dentin powder
AU - Kim, Jongryul
AU - Uchiyama, Toshikazu
AU - Carrilho, Marcela
AU - Agee, Kelli A.
AU - Mazzoni, Annalisa
AU - Breschi, Lorenzo
AU - Carvalho, Ricardo M.
AU - Tjäderhane, Leo
AU - Looney, Stephen Warwick
AU - Wimmer, Courtney
AU - Tezvergil-Mutluay, Arzu
AU - Tay, Franklin Chi Meng
AU - Pashley, David Henry
PY - 2010/8
Y1 - 2010/8
N2 - Objectives: The purposes of this work were to quantitate the affinity and binding capacity of chlorhexidine (CHX) digluconate to mineralized versus demineralized dentin powder and to determine how much debinding would result from rinsing with water, ethanol, hydroxyethylmethacrylate (HEMA) or 0.5 M NaCl in water. Methods: Dentin powder was made from coronal dentin of extracted human third molars. Standard amounts of dentin powder were tumbled with increasing concentrations of CHX (0-30 mM) for 30 min at 37 °C. After centrifuging the tubes, the supernatant was removed and the decrease in CHX concentration quantitated by UV-spectroscopy. CHX-treated dentin powder was resuspended in one of the four debinding solutions for 3 min. The amount of debound CHX in the solvents was also quantitated by UV-spectroscopy. Results: As the CHX concentration in the medium increased, the CHX binding to mineralized dentin powder also increased up to 6.8 μmol/g of dry dentin powder. Demineralized dentin powder took up significantly (p < 0.01) more CHX, reaching 30.1 μmol CHX/g of dry dentin powder. Debinding of CHX was in the order: HEMA < ethanol < 0.05 M NaCl < water. The highest CHX binding to demineralized dentin occurred at 30 mM (1.5 wt.%). Significance: As CHX is not debound by HEMA, it may remain bound to demineralized dentin during resin-dentin bonding. This may be responsible for the long-term efficacy of CHX as an MMP inhibitor in resin-dentin bonds.
AB - Objectives: The purposes of this work were to quantitate the affinity and binding capacity of chlorhexidine (CHX) digluconate to mineralized versus demineralized dentin powder and to determine how much debinding would result from rinsing with water, ethanol, hydroxyethylmethacrylate (HEMA) or 0.5 M NaCl in water. Methods: Dentin powder was made from coronal dentin of extracted human third molars. Standard amounts of dentin powder were tumbled with increasing concentrations of CHX (0-30 mM) for 30 min at 37 °C. After centrifuging the tubes, the supernatant was removed and the decrease in CHX concentration quantitated by UV-spectroscopy. CHX-treated dentin powder was resuspended in one of the four debinding solutions for 3 min. The amount of debound CHX in the solvents was also quantitated by UV-spectroscopy. Results: As the CHX concentration in the medium increased, the CHX binding to mineralized dentin powder also increased up to 6.8 μmol/g of dry dentin powder. Demineralized dentin powder took up significantly (p < 0.01) more CHX, reaching 30.1 μmol CHX/g of dry dentin powder. Debinding of CHX was in the order: HEMA < ethanol < 0.05 M NaCl < water. The highest CHX binding to demineralized dentin occurred at 30 mM (1.5 wt.%). Significance: As CHX is not debound by HEMA, it may remain bound to demineralized dentin during resin-dentin bonding. This may be responsible for the long-term efficacy of CHX as an MMP inhibitor in resin-dentin bonds.
KW - Binding
KW - Chlorhexidine
KW - Debinding
KW - Dentin bonding
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U2 - 10.1016/j.dental.2010.04.001
DO - 10.1016/j.dental.2010.04.001
M3 - Article
C2 - 20472280
AN - SCOPUS:77953687122
SN - 0109-5641
VL - 26
SP - 771
EP - 778
JO - Dental Materials
JF - Dental Materials
IS - 8
ER -