Chlorhexidine release and antibacterial properties of chlorhexidine- incorporated polymethyl methacrylate-based resin cement

N. Hiraishi, C. K.Y. Yiu, N. M. King, F. R. Tay

Research output: Contribution to journalArticlepeer-review

24 Scopus citations


This study evaluated chlorhexidine release from experimental, chlorhexidine-incorporated polymethyl methacrylate (PMMA)-based resin cements prepared from Super-Bond C&B (Sun Medical) and examined the antimicrobial activity against Streptococcus mutans and Enterococcus faecalis. Chlorhexidine diacetate was added into PMMA polymer to obtain chlorhexidine concentration of 0.0, 1.0, 2.0, 3.0, and 4.0 wt %. Chlorhexidine-incorporated, cured resin disks were immersed in distilled water at 37°C for 5 weeks, and the chlorhexidine release was analyzed by high-performance liquid chromatography. The antibacterial effect of freshly mixed resin cements was examined using the agar diffusion test. For the direct contact test, the wells (n = 6) of microtiter plates were coated with cements. The coated wells were aged up to 3 weeks prior to the placement of bacterial suspensions directly on cured cements. The 3.0 and 4.0% chlorhexidine-incorporated cement exhibited chlorhexidine release for 5 weeks; however, more than 98% of chlorhexidine was retained in resin matrix. No release was detected from the 1.0 and 2.0% incorporated cement at 1 week and 2 weeks, respectively. The agar diffusion test failed to detect antibacterial effects against Enterococcus faecalis, whereas the direct contact test revealed the antibacterial effect of 3.0 and 4.0% incorporated cements against each microbe for 2 weeks. The 3.0 and 4.0% chlorhexidine-incorporated resin cement possessed prolonged chlorhexidine release and antibacterial properties for 2 weeks.

Original languageEnglish (US)
Pages (from-to)134-140
Number of pages7
JournalJournal of Biomedical Materials Research - Part B Applied Biomaterials
Issue number1
StatePublished - Jul 2010


  • Agar diffusion test
  • Antibacterial effects
  • Chlorhexidine
  • Direct contact test
  • HPLC
  • Resin cement

ASJC Scopus subject areas

  • Biomaterials
  • Biomedical Engineering


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