Chlorhexidine stabilizes the adhesive interface: A 2-year in vitro study

Lorenzo Breschi, Annalisa Mazzoni, Fernando Nato, Marcela Carrilho, Erika Visintini, Leo Tjäderhane, Alessandra Ruggeri, Franklin Chi Meng Tay, Elettra De Stefano Dorigo, David Henry Pashley

Research output: Contribution to journalArticle

205 Citations (Scopus)

Abstract

Objectives: This study evaluated the role of endogenous dentin MMPs in auto-degradation of collagen fibrils within adhesive-bonded interfaces. The null hypotheses tested were that adhesive blends or chlorhexidine digluconate (CHX) application does not modify dentin MMPs activity and that CHX used as therapeutic primer does not improve the stability of adhesive interfaces over time. Methods: Zymograms of protein extracts from human dentin powder incubated with Adper Scotchbond 1XT (SB1XT) on untreated or 0.2-2% CHX-treated dentin were obtained to assay dentin MMPs activity. Microtensile bond strength and interfacial nanoleakage expression of SB1XT bonded interfaces (with or without CHX pre-treatment for 30 s on the etched surface) were analyzed immediately and after 2 years of storage in artificial saliva at 37 °C. Results: Zymograms showed that application of SB1XT to human dentin powder increases MMP-2 activity, while CHX pre-treatment inhibited all dentin gelatinolytic activity, irrespective from the tested concentration. CHX significantly lowered the loss of bond strength and nanoleakage seen in acid-etched resin-bonded dentin artificially aged for 2 years. Significance: The study demonstrates the active role of SB1XT in dentin MMP-2 activation and the efficacy of CHX inhibition of MMPs even if used at low concentration (0.2%).

Original languageEnglish (US)
Pages (from-to)320-325
Number of pages6
JournalDental Materials
Volume26
Issue number4
DOIs
StatePublished - Apr 1 2010

Fingerprint

Chlorhexidine
Dentin
varespladib methyl
Adhesives
Matrix Metalloproteinases
Powders
Collagen
Assays
Resins
Chemical activation
Proteins
Degradation
Artificial Saliva
Acids
In Vitro Techniques
chlorhexidine gluconate
Scotchbond

Keywords

  • Aging
  • Chlorhexidine
  • Dental bonding systems
  • Dentin
  • Hybrid layer

ASJC Scopus subject areas

  • Materials Science(all)
  • Dentistry(all)
  • Mechanics of Materials

Cite this

Breschi, L., Mazzoni, A., Nato, F., Carrilho, M., Visintini, E., Tjäderhane, L., ... Pashley, D. H. (2010). Chlorhexidine stabilizes the adhesive interface: A 2-year in vitro study. Dental Materials, 26(4), 320-325. https://doi.org/10.1016/j.dental.2009.11.153

Chlorhexidine stabilizes the adhesive interface : A 2-year in vitro study. / Breschi, Lorenzo; Mazzoni, Annalisa; Nato, Fernando; Carrilho, Marcela; Visintini, Erika; Tjäderhane, Leo; Ruggeri, Alessandra; Tay, Franklin Chi Meng; Dorigo, Elettra De Stefano; Pashley, David Henry.

In: Dental Materials, Vol. 26, No. 4, 01.04.2010, p. 320-325.

Research output: Contribution to journalArticle

Breschi, L, Mazzoni, A, Nato, F, Carrilho, M, Visintini, E, Tjäderhane, L, Ruggeri, A, Tay, FCM, Dorigo, EDS & Pashley, DH 2010, 'Chlorhexidine stabilizes the adhesive interface: A 2-year in vitro study', Dental Materials, vol. 26, no. 4, pp. 320-325. https://doi.org/10.1016/j.dental.2009.11.153
Breschi L, Mazzoni A, Nato F, Carrilho M, Visintini E, Tjäderhane L et al. Chlorhexidine stabilizes the adhesive interface: A 2-year in vitro study. Dental Materials. 2010 Apr 1;26(4):320-325. https://doi.org/10.1016/j.dental.2009.11.153
Breschi, Lorenzo ; Mazzoni, Annalisa ; Nato, Fernando ; Carrilho, Marcela ; Visintini, Erika ; Tjäderhane, Leo ; Ruggeri, Alessandra ; Tay, Franklin Chi Meng ; Dorigo, Elettra De Stefano ; Pashley, David Henry. / Chlorhexidine stabilizes the adhesive interface : A 2-year in vitro study. In: Dental Materials. 2010 ; Vol. 26, No. 4. pp. 320-325.
@article{fdaab7af5872488494aaf3e34307154b,
title = "Chlorhexidine stabilizes the adhesive interface: A 2-year in vitro study",
abstract = "Objectives: This study evaluated the role of endogenous dentin MMPs in auto-degradation of collagen fibrils within adhesive-bonded interfaces. The null hypotheses tested were that adhesive blends or chlorhexidine digluconate (CHX) application does not modify dentin MMPs activity and that CHX used as therapeutic primer does not improve the stability of adhesive interfaces over time. Methods: Zymograms of protein extracts from human dentin powder incubated with Adper Scotchbond 1XT (SB1XT) on untreated or 0.2-2{\%} CHX-treated dentin were obtained to assay dentin MMPs activity. Microtensile bond strength and interfacial nanoleakage expression of SB1XT bonded interfaces (with or without CHX pre-treatment for 30 s on the etched surface) were analyzed immediately and after 2 years of storage in artificial saliva at 37 °C. Results: Zymograms showed that application of SB1XT to human dentin powder increases MMP-2 activity, while CHX pre-treatment inhibited all dentin gelatinolytic activity, irrespective from the tested concentration. CHX significantly lowered the loss of bond strength and nanoleakage seen in acid-etched resin-bonded dentin artificially aged for 2 years. Significance: The study demonstrates the active role of SB1XT in dentin MMP-2 activation and the efficacy of CHX inhibition of MMPs even if used at low concentration (0.2{\%}).",
keywords = "Aging, Chlorhexidine, Dental bonding systems, Dentin, Hybrid layer",
author = "Lorenzo Breschi and Annalisa Mazzoni and Fernando Nato and Marcela Carrilho and Erika Visintini and Leo Tj{\"a}derhane and Alessandra Ruggeri and Tay, {Franklin Chi Meng} and Dorigo, {Elettra De Stefano} and Pashley, {David Henry}",
year = "2010",
month = "4",
day = "1",
doi = "10.1016/j.dental.2009.11.153",
language = "English (US)",
volume = "26",
pages = "320--325",
journal = "Dental Materials",
issn = "0109-5641",
publisher = "Elsevier Science",
number = "4",

}

TY - JOUR

T1 - Chlorhexidine stabilizes the adhesive interface

T2 - A 2-year in vitro study

AU - Breschi, Lorenzo

AU - Mazzoni, Annalisa

AU - Nato, Fernando

AU - Carrilho, Marcela

AU - Visintini, Erika

AU - Tjäderhane, Leo

AU - Ruggeri, Alessandra

AU - Tay, Franklin Chi Meng

AU - Dorigo, Elettra De Stefano

AU - Pashley, David Henry

PY - 2010/4/1

Y1 - 2010/4/1

N2 - Objectives: This study evaluated the role of endogenous dentin MMPs in auto-degradation of collagen fibrils within adhesive-bonded interfaces. The null hypotheses tested were that adhesive blends or chlorhexidine digluconate (CHX) application does not modify dentin MMPs activity and that CHX used as therapeutic primer does not improve the stability of adhesive interfaces over time. Methods: Zymograms of protein extracts from human dentin powder incubated with Adper Scotchbond 1XT (SB1XT) on untreated or 0.2-2% CHX-treated dentin were obtained to assay dentin MMPs activity. Microtensile bond strength and interfacial nanoleakage expression of SB1XT bonded interfaces (with or without CHX pre-treatment for 30 s on the etched surface) were analyzed immediately and after 2 years of storage in artificial saliva at 37 °C. Results: Zymograms showed that application of SB1XT to human dentin powder increases MMP-2 activity, while CHX pre-treatment inhibited all dentin gelatinolytic activity, irrespective from the tested concentration. CHX significantly lowered the loss of bond strength and nanoleakage seen in acid-etched resin-bonded dentin artificially aged for 2 years. Significance: The study demonstrates the active role of SB1XT in dentin MMP-2 activation and the efficacy of CHX inhibition of MMPs even if used at low concentration (0.2%).

AB - Objectives: This study evaluated the role of endogenous dentin MMPs in auto-degradation of collagen fibrils within adhesive-bonded interfaces. The null hypotheses tested were that adhesive blends or chlorhexidine digluconate (CHX) application does not modify dentin MMPs activity and that CHX used as therapeutic primer does not improve the stability of adhesive interfaces over time. Methods: Zymograms of protein extracts from human dentin powder incubated with Adper Scotchbond 1XT (SB1XT) on untreated or 0.2-2% CHX-treated dentin were obtained to assay dentin MMPs activity. Microtensile bond strength and interfacial nanoleakage expression of SB1XT bonded interfaces (with or without CHX pre-treatment for 30 s on the etched surface) were analyzed immediately and after 2 years of storage in artificial saliva at 37 °C. Results: Zymograms showed that application of SB1XT to human dentin powder increases MMP-2 activity, while CHX pre-treatment inhibited all dentin gelatinolytic activity, irrespective from the tested concentration. CHX significantly lowered the loss of bond strength and nanoleakage seen in acid-etched resin-bonded dentin artificially aged for 2 years. Significance: The study demonstrates the active role of SB1XT in dentin MMP-2 activation and the efficacy of CHX inhibition of MMPs even if used at low concentration (0.2%).

KW - Aging

KW - Chlorhexidine

KW - Dental bonding systems

KW - Dentin

KW - Hybrid layer

UR - http://www.scopus.com/inward/record.url?scp=76849106063&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=76849106063&partnerID=8YFLogxK

U2 - 10.1016/j.dental.2009.11.153

DO - 10.1016/j.dental.2009.11.153

M3 - Article

C2 - 20045177

AN - SCOPUS:76849106063

VL - 26

SP - 320

EP - 325

JO - Dental Materials

JF - Dental Materials

SN - 0109-5641

IS - 4

ER -