Chloride cotransporters as a molecular mechanism underlying spreading depolarization-induced dendritic beading

Annette B. Steffensen, Jeremy Sword, Deborah Croom, Sergei A Kirov, Nanna MacAulay

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Spreading depolarizations (SDs) are waves of sustained neuronal and glial depolarization that propagate massive disruptions of ion gradients through the brain. SD is associated with migraine aura and recently recognized as a novel mechanism of injury in stroke and brain trauma patients. SD leads to neuronal swelling as assessed in real time with two-photon laser scanning microscopy (2PLSM). Pyramidal neurons do not express aquaporins and thus display low inherent water permeability, yet SD rapidly induces focal swelling (beading) along the dendritic shaft by unidentified molecular mechanisms. To address this issue, we induced SD in murine hippocampal slices by focal KCl microinjection and visualized the ensuing beading of dendrites expressing EGFP by 2PLSM. We confirmed that dendritic beading failed to arise during large (100 mOsm) hyposmotic challenges, underscoring that neuronal swelling does not occur as a simple osmotic event. SD-induced dendritic beading was not prevented by pharmacological interference with the cytoskeleton, supporting the notion that dendritic beading may result entirely from excessive water influx. Dendritic beading was strictly dependent on the presence of Cl-, and, accordingly, combined blockade of Cl--coupled transporters led to a significant reduction in dendritic beading without interfering with SD. Furthermore, our in vivo data showed a strong inhibition of dendritic beading during pharmacological blockage of these cotransporters. We propose that SD-induced dendritic beading takes place as a consequence of the altered driving forces and thus activity for these cotransporters, which by transport of water during their translocation mechanism may generate dendritic beading independently of osmotic forces.

Original languageEnglish (US)
Pages (from-to)12172-12187
Number of pages16
JournalJournal of Neuroscience
Volume35
Issue number35
DOIs
StatePublished - Sep 2 2015

Fingerprint

Chlorides
Water
Pharmacology
Migraine with Aura
Aquaporins
Pyramidal Cells
Microinjections
Dendrites
Cytoskeleton
Photons
Confocal Microscopy
Neuroglia
Permeability
Stroke
Ions
Wounds and Injuries
Brain
Traumatic Brain Injury

Keywords

  • Cl-cotransporters
  • Cotransporters
  • Dendritic beading
  • Neuronal swelling
  • Spreading depression
  • Two-photon microscopy

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Chloride cotransporters as a molecular mechanism underlying spreading depolarization-induced dendritic beading. / Steffensen, Annette B.; Sword, Jeremy; Croom, Deborah; Kirov, Sergei A; MacAulay, Nanna.

In: Journal of Neuroscience, Vol. 35, No. 35, 02.09.2015, p. 12172-12187.

Research output: Contribution to journalArticle

Steffensen, Annette B. ; Sword, Jeremy ; Croom, Deborah ; Kirov, Sergei A ; MacAulay, Nanna. / Chloride cotransporters as a molecular mechanism underlying spreading depolarization-induced dendritic beading. In: Journal of Neuroscience. 2015 ; Vol. 35, No. 35. pp. 12172-12187.
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