Chlorpyrifos and chlorpyrifos oxon impair the transport of membrane bound organelles in rat cortical axons

Jie Gao, Sean X. Naughton, Wayne D. Beck, Caterina M. Hernandez, Guangyu Wu, Zhe Wei, Xiangkun Yang, Michael G. Bartlett, Alvin V Terry

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Chlorpyrifos (CPF) is an extensively used organophosphorus pesticide that has recently come under increasing scrutiny due to environmental health concerns particularly its association with neurodevelopmental defects. While the insecticidal actions and acute toxicity of CPF are attributed to its oxon metabolite (CPO) which potently inhibits the cholinergic enzyme acetylcholinesterase (AChE), there is significant evidence that CPF, CPO, and other organophosphates may affect a variety of neuronal targets and processes that are not directly related to AChE. Previously, in adult rat sciatic nerves ex vivo and postnatal neurons from rats in vitro we observed that CPF and CPO impaired the movements of vesicles and mitochondria in axons. Here, in embryonic neurons from rats in culture, we evaluated 24 h exposures to CPF and CPO across picomolar to micromolar concentrations for effects on fast axonal transport of membrane bound organelles (MBOs) that contained the amyloid precursor protein (APP) tagged with the fluorescent marker, Dendra2 (APPDendra2). The most notable observations of this study were concentration-dependent decreases in the velocity and percentage of MBOs moving in the anterograde direction, an increase in the number of stationary MBOs, and an increased frequency of pauses associated with both CPF and CPO. These effects occurred at concentrations that did not significantly inhibit AChE activity, they were not blocked by cholinergic receptor antagonists, and they were not associated with compromised cell viability. These effects of CPF and CPO may be significant given the importance of axonal transport to neuronal development as well the function of fully developed neurons.

Original languageEnglish (US)
Pages (from-to)111-123
Number of pages13
JournalNeuroToxicology
Volume62
DOIs
StatePublished - Sep 1 2017

Fingerprint

Chlorpyrifos
Organelles
Axons
Rats
Membranes
Acetylcholinesterase
Neurons
Axonal Transport
Mitochondria
Organophosphates
Environmental Health
Amyloid beta-Protein Precursor
Cholinergic Antagonists
Sciatic Nerve
Metabolites
O,O-diethyl O-3,5,6-trichloro-2-pyridyl phosphate
Pesticides
Cholinergic Agents
Toxicity
Cell Survival

Keywords

  • Amyloid precursor protein
  • Axonal transport
  • Gulf war illness
  • Neurodevelopment
  • Organophosphate
  • Pesticide

ASJC Scopus subject areas

  • Neuroscience(all)
  • Toxicology

Cite this

Chlorpyrifos and chlorpyrifos oxon impair the transport of membrane bound organelles in rat cortical axons. / Gao, Jie; Naughton, Sean X.; Beck, Wayne D.; Hernandez, Caterina M.; Wu, Guangyu; Wei, Zhe; Yang, Xiangkun; Bartlett, Michael G.; Terry, Alvin V.

In: NeuroToxicology, Vol. 62, 01.09.2017, p. 111-123.

Research output: Contribution to journalArticle

Gao, Jie ; Naughton, Sean X. ; Beck, Wayne D. ; Hernandez, Caterina M. ; Wu, Guangyu ; Wei, Zhe ; Yang, Xiangkun ; Bartlett, Michael G. ; Terry, Alvin V. / Chlorpyrifos and chlorpyrifos oxon impair the transport of membrane bound organelles in rat cortical axons. In: NeuroToxicology. 2017 ; Vol. 62. pp. 111-123.
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AU - Naughton, Sean X.

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