Abstract
The glycolytic enzyme phosphoglycerate kinase (PGK) is highly conserved throughout the evolutionary ladder. Using a previously cloned Saccharomyces cerevisiae PGK gene as hybridization probe, chromosomal localization and strain-specific variation of PGK gene in C. albicans have been examined. Separation of chromosome-size DNA fragments by pulse field gel electrophoresis followed by DNA- DNA hybridization analysis revealed that the PGK is encoded by a single copy gene located on the large chromosome of the commonly used laboratory strain ATCC 32354, as well as of several clinical isolates of C. albicans. Restriction fragment length analysis indicated that the PGK gene locus is highly polymorphic, and this strain-specific variation could be exploited to study intra-specific relatedness of clinical isolates.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 206-209 |
| Number of pages | 4 |
| Journal | Indian Journal of Experimental Biology |
| Volume | 31 |
| Issue number | 3 |
| State | Published - Jan 1 1993 |
Fingerprint
ASJC Scopus subject areas
- Biotechnology
- Molecular Biology
- Cell Biology
Cite this
Chromosomal localization of phosphoglycerate kinase (PGK) gene in Candida albicans using a heterologous probe. / Manavathu, Elias Kurian.
In: Indian Journal of Experimental Biology, Vol. 31, No. 3, 01.01.1993, p. 206-209.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Chromosomal localization of phosphoglycerate kinase (PGK) gene in Candida albicans using a heterologous probe.
AU - Manavathu, Elias Kurian
PY - 1993/1/1
Y1 - 1993/1/1
N2 - The glycolytic enzyme phosphoglycerate kinase (PGK) is highly conserved throughout the evolutionary ladder. Using a previously cloned Saccharomyces cerevisiae PGK gene as hybridization probe, chromosomal localization and strain-specific variation of PGK gene in C. albicans have been examined. Separation of chromosome-size DNA fragments by pulse field gel electrophoresis followed by DNA- DNA hybridization analysis revealed that the PGK is encoded by a single copy gene located on the large chromosome of the commonly used laboratory strain ATCC 32354, as well as of several clinical isolates of C. albicans. Restriction fragment length analysis indicated that the PGK gene locus is highly polymorphic, and this strain-specific variation could be exploited to study intra-specific relatedness of clinical isolates.
AB - The glycolytic enzyme phosphoglycerate kinase (PGK) is highly conserved throughout the evolutionary ladder. Using a previously cloned Saccharomyces cerevisiae PGK gene as hybridization probe, chromosomal localization and strain-specific variation of PGK gene in C. albicans have been examined. Separation of chromosome-size DNA fragments by pulse field gel electrophoresis followed by DNA- DNA hybridization analysis revealed that the PGK is encoded by a single copy gene located on the large chromosome of the commonly used laboratory strain ATCC 32354, as well as of several clinical isolates of C. albicans. Restriction fragment length analysis indicated that the PGK gene locus is highly polymorphic, and this strain-specific variation could be exploited to study intra-specific relatedness of clinical isolates.
UR - http://www.scopus.com/inward/record.url?scp=0027563485&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0027563485&partnerID=8YFLogxK
M3 - Article
C2 - 8099062
AN - SCOPUS:0027563485
VL - 31
SP - 206
EP - 209
JO - Journal of scientific & industrial research. C. Biological sciences
JF - Journal of scientific & industrial research. C. Biological sciences
SN - 0019-5189
IS - 3
ER -