Chronic Remote Ischemic Conditioning Is Cerebroprotective and Induces Vascular Remodeling in a VCID Model

Mohammad Badruzzaman Khan, Sherif Hafez, MD Nasrul Hoda, Babak Baban, Jesse Wagner, Mohamed E. Awad, Hasith Sangabathula, Stephen Haigh, Mohammed Elsayed Elsalanty, Jennifer L Waller, David C Hess

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Vascular contributions to cognitive impairment and dementia (VCID) make up 50% of the cases of dementia. The purpose of this study was to determine the effect of chronic remote ischemic conditioning (C-RIC) on improving long-term (6 months) outcomes and cerebral blood flow (CBF) and collateral formation in a mouse model of VCID. Adult C57BL/6J male mice (10 weeks) were randomly assigned to four different groups: (1) sham-bilateral carotid artery stenosis (BCAS), (2) BCAS + sham RIC, (3) BCAS+C-RIC for 1 month (1MO), and (4) BCAS+C-RIC-4 months (4MO). CBF, cognitive impairment, and functional outcomes were performed up for 6 months after BCAS surgery. The expression of CD31, α-SMA, and myelin basic protein (MBP) was assessed by immunohistochemistry (IHC). Additional set of mice were randomized to sham, BCAS, and BCAS+C-RIC. The cerebrovascular angioarchitecture was studied with micro-CT. RIC therapy for either 1 or 4 months significantly improved CBF, new collateral formation, functional and cognitive outcomes, and prevented white matter damage. There was no difference between C-RIC for 1 or 4 months; IHC studies at 6 months showed an increase in brain CD31 and α-SMA expression indicating increased angiogenesis and MBP indicating preservation of white matter in animals receiving RIC. One month of daily RIC is as effective as 4 months of daily RIC in improving CBF, angiogenesis, and long-term functional outcomes (6 months) in a VCID model. This suggests that 1 month of RIC is sufficient to reduce cognitive impairment and induce beneficial cerebrovascular remodeling.

Original languageEnglish (US)
Pages (from-to)51-63
Number of pages13
JournalTranslational Stroke Research
Volume9
Issue number1
DOIs
StatePublished - Feb 1 2018

Fingerprint

Carotid Stenosis
Cerebrovascular Circulation
Blood Vessels
Dementia
Myelin Basic Protein
Immunohistochemistry
Cognitive Dysfunction
Vascular Remodeling
Conditioning (Psychology)
Brain

Keywords

  • Angiogenesis, collateral remodeling, white matter degeneration
  • Cerebral blood flow (CBF)
  • Chronic remote ischemic conditioning (C-RIC)
  • Vascular contributions to cognitive impairment and dementia (VCID)

ASJC Scopus subject areas

  • Neuroscience(all)
  • Clinical Neurology
  • Cardiology and Cardiovascular Medicine

Cite this

Chronic Remote Ischemic Conditioning Is Cerebroprotective and Induces Vascular Remodeling in a VCID Model. / Khan, Mohammad Badruzzaman; Hafez, Sherif; Hoda, MD Nasrul; Baban, Babak; Wagner, Jesse; Awad, Mohamed E.; Sangabathula, Hasith; Haigh, Stephen; Elsalanty, Mohammed Elsayed; Waller, Jennifer L; Hess, David C.

In: Translational Stroke Research, Vol. 9, No. 1, 01.02.2018, p. 51-63.

Research output: Contribution to journalArticle

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