Clerodendron glandulosum.Coleb extract ameliorates high fat diet/fatty acid induced lipotoxicity in experimental models of non-alcoholic steatohepatitis

Ravirajsinh N. Jadeja, Menaka C. Thounaojam, Deven S. Dandekar, Ranjitsinh V. Devkar, A. V. Ramachandran

Research output: Contribution to journalArticle

21 Scopus citations

Abstract

This study evaluates the protective role of Clerodendron glandulosum.Coleb (CG) aqueous extract against high fat diet/fatty acid induced lipotoxicity in experimental models of non-alcoholic steatohepatitis (NASH). Supplementation of NASH mice with CG extract (1% and 3% in high fat diet for 16. weeks) prevented high fat diet induced elevation in liver enzymes, plasma and hepatic lipids, mitochondrial oxidative stress and compromised enzymatic and non-enzymatic antioxidant status and histopathological damage to hepatocytes. Furthermore, results from in vitro study indicates, addition of CG extract (20-200 μg/ml for 24. h) to HepG2 cells minimizes oleic acid induced lipid accumulation, higher lipid peroxidation, cytotoxicity and reduced cell viability. These in vivo and in vitro studies suggest that CG extract has the potential of preventing high fat/fatty acid induced NASH.

Original languageEnglish (US)
Pages (from-to)3424-3431
Number of pages8
JournalFood and Chemical Toxicology
Volume48
Issue number12
DOIs
StatePublished - Dec 1 2010
Externally publishedYes

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Keywords

  • Clerodendron glandulosum .Coleb
  • HepG2 cells
  • Non-alcoholic steatohepatitis
  • Oleic acid
  • Oxidative stress

ASJC Scopus subject areas

  • Food Science
  • Toxicology

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