Cloning and expression of a b0,+-like amino acid transporter functioning asa heterodimer with 4F2hc-instead of rBAT. A new candidate gene for cystinuria

D. Prasanna Rajan, Ramesh Kekuda, Wei Huang, Haiping Wang, Lawrence D Devoe, Frederick H. Leibach, Puttur D Prasad, Vadivel Ganapathy

Research output: Contribution to journalArticle

58 Citations (Scopus)

Abstract

We have cloned a transporter protein from rabbit small intestine, which, when coexpressed with the 4F2 heavy chain (4F2hc) in mammalian cells, induces a b0,+-like amino acid transport activity. This protein (4F2-lc6 for the sixth member of the 4F2 light chain family) consists of 487 amino acids and has 12 putative transmembrane domains. At the level of amino acid sequence, 4F2-lc6 shows significant homology (44% identity) to the other five known members of the 4F2 light chain family, namely LAT1 (4F2-lc1), y+LAT1 (4F2- lc2), y+LAT2 (4F2-lc3), xCT (4F2-lc4), and LAT2 (4F2-lc5). The 4F2hc/4F2-lc6 complex-mediated transport process is Na+-independent and exhibits high affinity for neutral and cationic amino acids and cystine. These characteristics are similar to those of the b0,+-like amino acid transport activity previously shown to be associated with rBAT (protein related to b0,+ amino acid transport system). However, the newly cloned 4F2-lc6 does not interact with rBAT. This is the first report of the existence of ab0,+ like amino acid transport process that is independent of rBAT. 4F2-lc6 is expressed predominantly in the small intestine and kidney. Based on the characteristics of the transport process mediated by the 4F2hc/4F2-lc6 complex and the expression pattern of 4F2-lc6 in mammalian tissues, we suggest that 4F2-lc6 is a new candidate gene for cystinuria.

Original languageEnglish (US)
Pages (from-to)29005-29010
Number of pages6
JournalJournal of Biological Chemistry
Volume274
Issue number41
DOIs
StatePublished - Oct 8 1999

Fingerprint

CD98 Heavy Chain Antigens
Cystinuria
Amino Acid Transport Systems
Cloning
CD98 Light Chains Antigens
Organism Cloning
Genes
Amino Acids
Small Intestine
Neutral Amino Acids
Proteins
Cystine
Amino Acid Sequence
Rabbits
Kidney
Cells
Tissue

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Cloning and expression of a b0,+-like amino acid transporter functioning asa heterodimer with 4F2hc-instead of rBAT. A new candidate gene for cystinuria. / Rajan, D. Prasanna; Kekuda, Ramesh; Huang, Wei; Wang, Haiping; Devoe, Lawrence D; Leibach, Frederick H.; Prasad, Puttur D; Ganapathy, Vadivel.

In: Journal of Biological Chemistry, Vol. 274, No. 41, 08.10.1999, p. 29005-29010.

Research output: Contribution to journalArticle

Rajan, D. Prasanna ; Kekuda, Ramesh ; Huang, Wei ; Wang, Haiping ; Devoe, Lawrence D ; Leibach, Frederick H. ; Prasad, Puttur D ; Ganapathy, Vadivel. / Cloning and expression of a b0,+-like amino acid transporter functioning asa heterodimer with 4F2hc-instead of rBAT. A new candidate gene for cystinuria. In: Journal of Biological Chemistry. 1999 ; Vol. 274, No. 41. pp. 29005-29010.
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abstract = "We have cloned a transporter protein from rabbit small intestine, which, when coexpressed with the 4F2 heavy chain (4F2hc) in mammalian cells, induces a b0,+-like amino acid transport activity. This protein (4F2-lc6 for the sixth member of the 4F2 light chain family) consists of 487 amino acids and has 12 putative transmembrane domains. At the level of amino acid sequence, 4F2-lc6 shows significant homology (44{\%} identity) to the other five known members of the 4F2 light chain family, namely LAT1 (4F2-lc1), y+LAT1 (4F2- lc2), y+LAT2 (4F2-lc3), xCT (4F2-lc4), and LAT2 (4F2-lc5). The 4F2hc/4F2-lc6 complex-mediated transport process is Na+-independent and exhibits high affinity for neutral and cationic amino acids and cystine. These characteristics are similar to those of the b0,+-like amino acid transport activity previously shown to be associated with rBAT (protein related to b0,+ amino acid transport system). However, the newly cloned 4F2-lc6 does not interact with rBAT. This is the first report of the existence of ab0,+ like amino acid transport process that is independent of rBAT. 4F2-lc6 is expressed predominantly in the small intestine and kidney. Based on the characteristics of the transport process mediated by the 4F2hc/4F2-lc6 complex and the expression pattern of 4F2-lc6 in mammalian tissues, we suggest that 4F2-lc6 is a new candidate gene for cystinuria.",
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T1 - Cloning and expression of a b0,+-like amino acid transporter functioning asa heterodimer with 4F2hc-instead of rBAT. A new candidate gene for cystinuria

AU - Rajan, D. Prasanna

AU - Kekuda, Ramesh

AU - Huang, Wei

AU - Wang, Haiping

AU - Devoe, Lawrence D

AU - Leibach, Frederick H.

AU - Prasad, Puttur D

AU - Ganapathy, Vadivel

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AB - We have cloned a transporter protein from rabbit small intestine, which, when coexpressed with the 4F2 heavy chain (4F2hc) in mammalian cells, induces a b0,+-like amino acid transport activity. This protein (4F2-lc6 for the sixth member of the 4F2 light chain family) consists of 487 amino acids and has 12 putative transmembrane domains. At the level of amino acid sequence, 4F2-lc6 shows significant homology (44% identity) to the other five known members of the 4F2 light chain family, namely LAT1 (4F2-lc1), y+LAT1 (4F2- lc2), y+LAT2 (4F2-lc3), xCT (4F2-lc4), and LAT2 (4F2-lc5). The 4F2hc/4F2-lc6 complex-mediated transport process is Na+-independent and exhibits high affinity for neutral and cationic amino acids and cystine. These characteristics are similar to those of the b0,+-like amino acid transport activity previously shown to be associated with rBAT (protein related to b0,+ amino acid transport system). However, the newly cloned 4F2-lc6 does not interact with rBAT. This is the first report of the existence of ab0,+ like amino acid transport process that is independent of rBAT. 4F2-lc6 is expressed predominantly in the small intestine and kidney. Based on the characteristics of the transport process mediated by the 4F2hc/4F2-lc6 complex and the expression pattern of 4F2-lc6 in mammalian tissues, we suggest that 4F2-lc6 is a new candidate gene for cystinuria.

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