Cloning of murine tissue factor and regulation of gene expression by transforming growth factor type β1

G. Ranganathan, S. P. Blatti, M. Subramaniam, D. N. Fass, Nita Jane Maihle, M. J. Getz

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

We have cloned a serum- and cycloheximide-inducible mRNA from AKR-2B murine fibroblasts which encodes a protein with significant sequence similarity to human tissue factor, a cellular initiator of the blood coagulation cascade. Information derived from this clone was used to establish the presence of a virtually identical sequence in mouse brain. Most importantly, cDNA-directed expression in a quail fibroblast cell line produced high levels of tissue factor procoagulant activity, confirming the identity of this protein as murine tissue factor. Additional studies demonstrate that transforming growth factor type β1 stimulates tissue factor gene transcription and is a potent inducer of tissue factor procoagulant activity in fibroblasts. Other tested mitogens such as platelet-derived growth factor, epidermal growth factor, and insulin were weak inducers. These results may reflect a role for transforming growth factor β1 in the maintenance of hemostasis or, alternatively, a role for tissue factor in cellular functions unrelated to blood coagulation.

Original languageEnglish (US)
Pages (from-to)496-501
Number of pages6
JournalJournal of Biological Chemistry
Volume266
Issue number1
StatePublished - Jan 31 1991

Fingerprint

Cloning
Gene Expression Regulation
Thromboplastin
Transforming Growth Factors
Gene expression
Organism Cloning
Fibroblasts
Blood Coagulation
Coagulation
Blood
Quail
Platelet-Derived Growth Factor
Transcription
Cycloheximide
Hemostasis
Mitogens
Epidermal Growth Factor
Brain
Proteins
Complementary DNA

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Ranganathan, G., Blatti, S. P., Subramaniam, M., Fass, D. N., Maihle, N. J., & Getz, M. J. (1991). Cloning of murine tissue factor and regulation of gene expression by transforming growth factor type β1. Journal of Biological Chemistry, 266(1), 496-501.

Cloning of murine tissue factor and regulation of gene expression by transforming growth factor type β1. / Ranganathan, G.; Blatti, S. P.; Subramaniam, M.; Fass, D. N.; Maihle, Nita Jane; Getz, M. J.

In: Journal of Biological Chemistry, Vol. 266, No. 1, 31.01.1991, p. 496-501.

Research output: Contribution to journalArticle

Ranganathan, G, Blatti, SP, Subramaniam, M, Fass, DN, Maihle, NJ & Getz, MJ 1991, 'Cloning of murine tissue factor and regulation of gene expression by transforming growth factor type β1', Journal of Biological Chemistry, vol. 266, no. 1, pp. 496-501.
Ranganathan G, Blatti SP, Subramaniam M, Fass DN, Maihle NJ, Getz MJ. Cloning of murine tissue factor and regulation of gene expression by transforming growth factor type β1. Journal of Biological Chemistry. 1991 Jan 31;266(1):496-501.
Ranganathan, G. ; Blatti, S. P. ; Subramaniam, M. ; Fass, D. N. ; Maihle, Nita Jane ; Getz, M. J. / Cloning of murine tissue factor and regulation of gene expression by transforming growth factor type β1. In: Journal of Biological Chemistry. 1991 ; Vol. 266, No. 1. pp. 496-501.
@article{bffa3405fabb48b990019b657ddf69ce,
title = "Cloning of murine tissue factor and regulation of gene expression by transforming growth factor type β1",
abstract = "We have cloned a serum- and cycloheximide-inducible mRNA from AKR-2B murine fibroblasts which encodes a protein with significant sequence similarity to human tissue factor, a cellular initiator of the blood coagulation cascade. Information derived from this clone was used to establish the presence of a virtually identical sequence in mouse brain. Most importantly, cDNA-directed expression in a quail fibroblast cell line produced high levels of tissue factor procoagulant activity, confirming the identity of this protein as murine tissue factor. Additional studies demonstrate that transforming growth factor type β1 stimulates tissue factor gene transcription and is a potent inducer of tissue factor procoagulant activity in fibroblasts. Other tested mitogens such as platelet-derived growth factor, epidermal growth factor, and insulin were weak inducers. These results may reflect a role for transforming growth factor β1 in the maintenance of hemostasis or, alternatively, a role for tissue factor in cellular functions unrelated to blood coagulation.",
author = "G. Ranganathan and Blatti, {S. P.} and M. Subramaniam and Fass, {D. N.} and Maihle, {Nita Jane} and Getz, {M. J.}",
year = "1991",
month = "1",
day = "31",
language = "English (US)",
volume = "266",
pages = "496--501",
journal = "Journal of Biological Chemistry",
issn = "0021-9258",
publisher = "American Society for Biochemistry and Molecular Biology Inc.",
number = "1",

}

TY - JOUR

T1 - Cloning of murine tissue factor and regulation of gene expression by transforming growth factor type β1

AU - Ranganathan, G.

AU - Blatti, S. P.

AU - Subramaniam, M.

AU - Fass, D. N.

AU - Maihle, Nita Jane

AU - Getz, M. J.

PY - 1991/1/31

Y1 - 1991/1/31

N2 - We have cloned a serum- and cycloheximide-inducible mRNA from AKR-2B murine fibroblasts which encodes a protein with significant sequence similarity to human tissue factor, a cellular initiator of the blood coagulation cascade. Information derived from this clone was used to establish the presence of a virtually identical sequence in mouse brain. Most importantly, cDNA-directed expression in a quail fibroblast cell line produced high levels of tissue factor procoagulant activity, confirming the identity of this protein as murine tissue factor. Additional studies demonstrate that transforming growth factor type β1 stimulates tissue factor gene transcription and is a potent inducer of tissue factor procoagulant activity in fibroblasts. Other tested mitogens such as platelet-derived growth factor, epidermal growth factor, and insulin were weak inducers. These results may reflect a role for transforming growth factor β1 in the maintenance of hemostasis or, alternatively, a role for tissue factor in cellular functions unrelated to blood coagulation.

AB - We have cloned a serum- and cycloheximide-inducible mRNA from AKR-2B murine fibroblasts which encodes a protein with significant sequence similarity to human tissue factor, a cellular initiator of the blood coagulation cascade. Information derived from this clone was used to establish the presence of a virtually identical sequence in mouse brain. Most importantly, cDNA-directed expression in a quail fibroblast cell line produced high levels of tissue factor procoagulant activity, confirming the identity of this protein as murine tissue factor. Additional studies demonstrate that transforming growth factor type β1 stimulates tissue factor gene transcription and is a potent inducer of tissue factor procoagulant activity in fibroblasts. Other tested mitogens such as platelet-derived growth factor, epidermal growth factor, and insulin were weak inducers. These results may reflect a role for transforming growth factor β1 in the maintenance of hemostasis or, alternatively, a role for tissue factor in cellular functions unrelated to blood coagulation.

UR - http://www.scopus.com/inward/record.url?scp=0025957232&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025957232&partnerID=8YFLogxK

M3 - Article

VL - 266

SP - 496

EP - 501

JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

SN - 0021-9258

IS - 1

ER -