Clustered DNA damages induced by x rays in human cells

Betsy M. Sutherland, Paula V. Bennett, John C. Sutherland, Jacques Laval

Research output: Contribution to journalArticle

144 Scopus citations

Abstract

Although DNA DSBs are known to be important in producing the damaging effects of ionizing radiation in cells, bistranded clustered DNA damages - two or more oxidized bases, abasic sites or strand breaks on opposing DNA strands within a few helical turns - are postulated to be difficult to repair and thus to be critical radiation-induced lesions. Gamma rays can induce clustered damages in DNA in solution, and high-energy iron ions produce DSBs and oxidized pyrimidine clusters in human cells, but it was not known whether sparsely ionizing radiation can produce clustered damages in mammalian cells. We show here that x rays induce abasic clusters, oxidized pyrimidine clusters, and oxidized purine clusters in DNA in human cells. Non-DSB clustered damages comprise about 70% of the complex lesions produced in cells. The relative levels of specific cluster classes depend on the environment of the DNA.

Original languageEnglish (US)
Pages (from-to)611-616
Number of pages6
JournalRadiation research
Volume157
Issue number6
DOIs
StatePublished - Jan 1 2002
Externally publishedYes

    Fingerprint

ASJC Scopus subject areas

  • Biophysics
  • Radiation
  • Radiology Nuclear Medicine and imaging

Cite this