Clustered DNA damages induced in human hematopoietic cells by low doses of ionizing radiation.

Betsy M. Sutherland, Paula V. Bennett, Nela Cintron-Torres, Megumi Hada, John Trunk, Denise Monteleone, John C. Sutherland, Jacques Laval, Marisha Stanislaus, Alan Gewirtz

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Ionizing radiation induces clusters of DNA damages--oxidized bases, abasic sites and strand breaks--on opposing strands within a few helical turns. Such damages have been postulated to be difficult to repair, as are double strand breaks (one type of cluster). We have shown that low doses of low and high linear energy transfer (LET) radiation induce such damage clusters in human cells. In human cells, DSB are about 30% of the total of complex damages, and the levels of DSBs and oxidized pyrimidine clusters are similar. The dose responses for cluster induction in cells can be described by a linear relationship, implying that even low doses of ionizing radiation can produce clustered damages. Studies are in progress to determine whether clusters can be produced by mechanisms other than ionizing radiation, as well as the levels of various cluster types formed by low and high LET radiation.

Original languageEnglish (US)
JournalJournal of Radiation Research
Volume43 Suppl
StatePublished - Jan 1 2002
Externally publishedYes

Fingerprint

Ionizing Radiation
ionizing radiation
DNA damage
DNA Damage
Linear Energy Transfer
deoxyribonucleic acid
energy transfer
damage
dosage
cells
Radiation
strands
pyrimidines
linear energy transfer (LET)
dose response
radiation
induction
1,2-di-(4-sulfamidophenyl)-4-butylpyrazolidine-3,5-dione

ASJC Scopus subject areas

  • Radiation
  • Radiology Nuclear Medicine and imaging
  • Health, Toxicology and Mutagenesis

Cite this

Sutherland, B. M., Bennett, P. V., Cintron-Torres, N., Hada, M., Trunk, J., Monteleone, D., ... Gewirtz, A. (2002). Clustered DNA damages induced in human hematopoietic cells by low doses of ionizing radiation. Journal of Radiation Research, 43 Suppl.

Clustered DNA damages induced in human hematopoietic cells by low doses of ionizing radiation. / Sutherland, Betsy M.; Bennett, Paula V.; Cintron-Torres, Nela; Hada, Megumi; Trunk, John; Monteleone, Denise; Sutherland, John C.; Laval, Jacques; Stanislaus, Marisha; Gewirtz, Alan.

In: Journal of Radiation Research, Vol. 43 Suppl, 01.01.2002.

Research output: Contribution to journalArticle

Sutherland, BM, Bennett, PV, Cintron-Torres, N, Hada, M, Trunk, J, Monteleone, D, Sutherland, JC, Laval, J, Stanislaus, M & Gewirtz, A 2002, 'Clustered DNA damages induced in human hematopoietic cells by low doses of ionizing radiation.', Journal of Radiation Research, vol. 43 Suppl.
Sutherland BM, Bennett PV, Cintron-Torres N, Hada M, Trunk J, Monteleone D et al. Clustered DNA damages induced in human hematopoietic cells by low doses of ionizing radiation. Journal of Radiation Research. 2002 Jan 1;43 Suppl.
Sutherland, Betsy M. ; Bennett, Paula V. ; Cintron-Torres, Nela ; Hada, Megumi ; Trunk, John ; Monteleone, Denise ; Sutherland, John C. ; Laval, Jacques ; Stanislaus, Marisha ; Gewirtz, Alan. / Clustered DNA damages induced in human hematopoietic cells by low doses of ionizing radiation. In: Journal of Radiation Research. 2002 ; Vol. 43 Suppl.
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AU - Monteleone, Denise

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