Collagen IV contributes to nitric oxide-induced angiogenesis of lung endothelial cells

Huafang Wang, Yunchao Su

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Nitric oxide (NO) mediates endothelial angiogenesis via inducing the expression of integrin ανβ3. During angiogenesis, endothelial cells adhere to and migrate into the extracellular matrix through integrins. Collagen IV binds to integrin ανβ3, leading to integrin activation, which affects a number of signaling processes in endothelial cells. In the present study, we evaluated the role of collagen IV in NO-induced angiogenesis. We found that NO donor 2,2'-(hydroxynitrosohydrazino)bis-ethanamine (NOC-18) causes increases in collagen IV mRNA and protein in lung endothelial cells and collagen IV release into the medium. Addition of collagen IV into the coating of endothelial culture increases endothelial monolayer wound repair, proliferation, and tube formation. Inhibition of collagen IV synthesis using gene silencing attenuates NOC- 18-induced increases in monolayer wound repair, cell proliferation, and tube formation as well as in the phosphorylation of focal adhesion kinase (FAK). Integrin blocking antibody LM609 prevents NOC-18- induced increase in endothelial monolayer wound repair. Inhibition of protein kinase G (PKG) using the specific PKG inhibitor KT5823 or PKG small interfering RNA prevents NOC-18-induced increases in collagen IV protein and mRNA and endothelial angiogenesis. Together, these results indicate that NO promotes collagen IV synthesis via a PKG signaling pathway and that the increase in collagen IV synthesis contributes to NO-induced angiogenesis of lung endothelial cells through integrin-FAK signaling. Manipulation of collagen IV could be a novel approach for the prevention and treatment of diseases such as alveolar capillary dysplasia, severe pulmonary arterial hypertension, and tumor invasion.

Original languageEnglish (US)
Pages (from-to)C979-C988
JournalAmerican Journal of Physiology - Cell Physiology
Volume300
Issue number5
DOIs
StatePublished - May 1 2011

Fingerprint

Nitric Oxide
Collagen
Endothelial Cells
Lung
Integrins
Cyclic GMP-Dependent Protein Kinases
Focal Adhesion Protein-Tyrosine Kinases
Wounds and Injuries
Messenger RNA
Blocking Antibodies
Nitric Oxide Donors
Gene Silencing
Protein Kinase Inhibitors
Pulmonary Hypertension
Small Interfering RNA
Extracellular Matrix
Proteins
Phosphorylation
Cell Proliferation
NOC 18

Keywords

  • Angiogenic
  • Endothelium
  • Extracellular matrix
  • NOC-18

ASJC Scopus subject areas

  • Physiology
  • Cell Biology

Cite this

Collagen IV contributes to nitric oxide-induced angiogenesis of lung endothelial cells. / Wang, Huafang; Su, Yunchao.

In: American Journal of Physiology - Cell Physiology, Vol. 300, No. 5, 01.05.2011, p. C979-C988.

Research output: Contribution to journalArticle

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