Collagen-phosphorylcholine interpenetrating network hydrogels as corneal substitutes

Wenguang Liu, Chao Deng, Christopher R. McLaughlin, Per Fagerholm, Neil S. Lagali, Belinda Heyne, Juan C. Scaiano, Mitchell Aaron Watsky, Yasuhiro Kato, Rejean Munger, Naoshi Shinozaki, Fengfu Li, May Griffith

Research output: Contribution to journalArticle

119 Citations (Scopus)

Abstract

A biointeractive collagen-phospholipid corneal substitute was fabricated from interpenetrating polymeric networks comprising 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide and N-hydroxysuccinimide crosslinked porcine atelocollagen, and poly(ethylene glycol) diacrylate crosslinked 2-methacryloyloxyethyl phosphorylcholine (MPC). The resulting hydrogels showed an overall increase in mechanical strength beyond that of either original component and enhanced stability against enzymatic digestion (by collagenase) or UV degradation. More strikingly, these hydrogels retained the full biointeractive, cell friendly properties of collagen in promoting corneal cell and nerve in-growth and regeneration (despite MPC's known anti-adhesive properties). Measurements of refractive indices, white light transmission and backscatter showed the optical properties of collagen-MPC are comparable or superior to those of the human cornea. In addition, the glucose and albumin permeability were comparable to those of human corneas. Twelve-month post-implantation results of collagen-MPC hydrogels into mini-pigs showed regeneration of corneal tissue (epithelium, stroma) as well as the tear film and sensory nerves. We also show that porcine collagen can be substituted with recombinant human collagen, resulting in a fully-synthetic implant that is free from the potential risks of disease transmission (e.g. prions) present in animal source materials.

Original languageEnglish (US)
Pages (from-to)1551-1559
Number of pages9
JournalBiomaterials
Volume30
Issue number8
DOIs
StatePublished - Mar 1 2009

Fingerprint

Hydrogels
Interpenetrating polymer networks
Phosphorylcholine
Collagen
Swine
Cornea
Regeneration
Carbodiimides
Refractometry
Corneal Epithelium
Prions
Phospholipids
Collagenases
Light transmission
Tears
Adhesives
Polyethylene glycols
Strength of materials
Glucose
Digestion

Keywords

  • Biomedical regeneration
  • Cornea substitute
  • Phospholipid
  • Recombinant collagen
  • Tissue engineering

ASJC Scopus subject areas

  • Bioengineering
  • Ceramics and Composites
  • Biophysics
  • Biomaterials
  • Mechanics of Materials

Cite this

Liu, W., Deng, C., McLaughlin, C. R., Fagerholm, P., Lagali, N. S., Heyne, B., ... Griffith, M. (2009). Collagen-phosphorylcholine interpenetrating network hydrogels as corneal substitutes. Biomaterials, 30(8), 1551-1559. https://doi.org/10.1016/j.biomaterials.2008.11.022

Collagen-phosphorylcholine interpenetrating network hydrogels as corneal substitutes. / Liu, Wenguang; Deng, Chao; McLaughlin, Christopher R.; Fagerholm, Per; Lagali, Neil S.; Heyne, Belinda; Scaiano, Juan C.; Watsky, Mitchell Aaron; Kato, Yasuhiro; Munger, Rejean; Shinozaki, Naoshi; Li, Fengfu; Griffith, May.

In: Biomaterials, Vol. 30, No. 8, 01.03.2009, p. 1551-1559.

Research output: Contribution to journalArticle

Liu, W, Deng, C, McLaughlin, CR, Fagerholm, P, Lagali, NS, Heyne, B, Scaiano, JC, Watsky, MA, Kato, Y, Munger, R, Shinozaki, N, Li, F & Griffith, M 2009, 'Collagen-phosphorylcholine interpenetrating network hydrogels as corneal substitutes', Biomaterials, vol. 30, no. 8, pp. 1551-1559. https://doi.org/10.1016/j.biomaterials.2008.11.022
Liu W, Deng C, McLaughlin CR, Fagerholm P, Lagali NS, Heyne B et al. Collagen-phosphorylcholine interpenetrating network hydrogels as corneal substitutes. Biomaterials. 2009 Mar 1;30(8):1551-1559. https://doi.org/10.1016/j.biomaterials.2008.11.022
Liu, Wenguang ; Deng, Chao ; McLaughlin, Christopher R. ; Fagerholm, Per ; Lagali, Neil S. ; Heyne, Belinda ; Scaiano, Juan C. ; Watsky, Mitchell Aaron ; Kato, Yasuhiro ; Munger, Rejean ; Shinozaki, Naoshi ; Li, Fengfu ; Griffith, May. / Collagen-phosphorylcholine interpenetrating network hydrogels as corneal substitutes. In: Biomaterials. 2009 ; Vol. 30, No. 8. pp. 1551-1559.
@article{ded01cc26f9445238c8e56fe8b18a59d,
title = "Collagen-phosphorylcholine interpenetrating network hydrogels as corneal substitutes",
abstract = "A biointeractive collagen-phospholipid corneal substitute was fabricated from interpenetrating polymeric networks comprising 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide and N-hydroxysuccinimide crosslinked porcine atelocollagen, and poly(ethylene glycol) diacrylate crosslinked 2-methacryloyloxyethyl phosphorylcholine (MPC). The resulting hydrogels showed an overall increase in mechanical strength beyond that of either original component and enhanced stability against enzymatic digestion (by collagenase) or UV degradation. More strikingly, these hydrogels retained the full biointeractive, cell friendly properties of collagen in promoting corneal cell and nerve in-growth and regeneration (despite MPC's known anti-adhesive properties). Measurements of refractive indices, white light transmission and backscatter showed the optical properties of collagen-MPC are comparable or superior to those of the human cornea. In addition, the glucose and albumin permeability were comparable to those of human corneas. Twelve-month post-implantation results of collagen-MPC hydrogels into mini-pigs showed regeneration of corneal tissue (epithelium, stroma) as well as the tear film and sensory nerves. We also show that porcine collagen can be substituted with recombinant human collagen, resulting in a fully-synthetic implant that is free from the potential risks of disease transmission (e.g. prions) present in animal source materials.",
keywords = "Biomedical regeneration, Cornea substitute, Phospholipid, Recombinant collagen, Tissue engineering",
author = "Wenguang Liu and Chao Deng and McLaughlin, {Christopher R.} and Per Fagerholm and Lagali, {Neil S.} and Belinda Heyne and Scaiano, {Juan C.} and Watsky, {Mitchell Aaron} and Yasuhiro Kato and Rejean Munger and Naoshi Shinozaki and Fengfu Li and May Griffith",
year = "2009",
month = "3",
day = "1",
doi = "10.1016/j.biomaterials.2008.11.022",
language = "English (US)",
volume = "30",
pages = "1551--1559",
journal = "Biomaterials",
issn = "0142-9612",
publisher = "Elsevier BV",
number = "8",

}

TY - JOUR

T1 - Collagen-phosphorylcholine interpenetrating network hydrogels as corneal substitutes

AU - Liu, Wenguang

AU - Deng, Chao

AU - McLaughlin, Christopher R.

AU - Fagerholm, Per

AU - Lagali, Neil S.

AU - Heyne, Belinda

AU - Scaiano, Juan C.

AU - Watsky, Mitchell Aaron

AU - Kato, Yasuhiro

AU - Munger, Rejean

AU - Shinozaki, Naoshi

AU - Li, Fengfu

AU - Griffith, May

PY - 2009/3/1

Y1 - 2009/3/1

N2 - A biointeractive collagen-phospholipid corneal substitute was fabricated from interpenetrating polymeric networks comprising 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide and N-hydroxysuccinimide crosslinked porcine atelocollagen, and poly(ethylene glycol) diacrylate crosslinked 2-methacryloyloxyethyl phosphorylcholine (MPC). The resulting hydrogels showed an overall increase in mechanical strength beyond that of either original component and enhanced stability against enzymatic digestion (by collagenase) or UV degradation. More strikingly, these hydrogels retained the full biointeractive, cell friendly properties of collagen in promoting corneal cell and nerve in-growth and regeneration (despite MPC's known anti-adhesive properties). Measurements of refractive indices, white light transmission and backscatter showed the optical properties of collagen-MPC are comparable or superior to those of the human cornea. In addition, the glucose and albumin permeability were comparable to those of human corneas. Twelve-month post-implantation results of collagen-MPC hydrogels into mini-pigs showed regeneration of corneal tissue (epithelium, stroma) as well as the tear film and sensory nerves. We also show that porcine collagen can be substituted with recombinant human collagen, resulting in a fully-synthetic implant that is free from the potential risks of disease transmission (e.g. prions) present in animal source materials.

AB - A biointeractive collagen-phospholipid corneal substitute was fabricated from interpenetrating polymeric networks comprising 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide and N-hydroxysuccinimide crosslinked porcine atelocollagen, and poly(ethylene glycol) diacrylate crosslinked 2-methacryloyloxyethyl phosphorylcholine (MPC). The resulting hydrogels showed an overall increase in mechanical strength beyond that of either original component and enhanced stability against enzymatic digestion (by collagenase) or UV degradation. More strikingly, these hydrogels retained the full biointeractive, cell friendly properties of collagen in promoting corneal cell and nerve in-growth and regeneration (despite MPC's known anti-adhesive properties). Measurements of refractive indices, white light transmission and backscatter showed the optical properties of collagen-MPC are comparable or superior to those of the human cornea. In addition, the glucose and albumin permeability were comparable to those of human corneas. Twelve-month post-implantation results of collagen-MPC hydrogels into mini-pigs showed regeneration of corneal tissue (epithelium, stroma) as well as the tear film and sensory nerves. We also show that porcine collagen can be substituted with recombinant human collagen, resulting in a fully-synthetic implant that is free from the potential risks of disease transmission (e.g. prions) present in animal source materials.

KW - Biomedical regeneration

KW - Cornea substitute

KW - Phospholipid

KW - Recombinant collagen

KW - Tissue engineering

UR - http://www.scopus.com/inward/record.url?scp=58249089265&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=58249089265&partnerID=8YFLogxK

U2 - 10.1016/j.biomaterials.2008.11.022

DO - 10.1016/j.biomaterials.2008.11.022

M3 - Article

C2 - 19097643

AN - SCOPUS:58249089265

VL - 30

SP - 1551

EP - 1559

JO - Biomaterials

JF - Biomaterials

SN - 0142-9612

IS - 8

ER -