@article{2d9b541c826e454e9e0e7c8a29047bf7,
title = "Comparison of parallel line skin test assay (PLST) and ELISA inhibition (EI) methods for determination of relative potency of polymerized grass and ragweed antigenic extracts",
abstract = "Relative potency estimates were performed by parallel line skin test assay (PLST) and ELISA inhibition methods for three polymerized allergen extracts (Bermuda grass, orchard grass, and a copolymer of short and giant ragweed) versus four unmodified RAST standardized reference extracts (Bermuda grass, orchard grass, and giant and short ragweed) in nine subjects. One subject experienced a systemic reaction, requiring treatment at the end of the PLST assay. Another subject had a systemic reaction during limited skin testing performed approximately 72 hours after completion of PLST. Relative potency values for the polymerized extracts obtained by PLST were much lower than those obtained by ELISA inhibition, but results were significantly (r = 0.95; p < 0.01) correlated. Because polymerized allergen extracts are designed to be hypoaller genie, a skin test assay may underestimate their potency relative to an unmodified reference extract.",
author = "Dolen, {William K.} and Miller, {Brian T.} and Ledoux, {Robert L.} and Seltzer, {James M.} and Wiener, {Matthew B.} and Selner, {John C.} and Nelson, {Harold S.}",
note = "Funding Information: From the *Allergy-Immunology Service, Fitzsimons Army Medical Center, Aurora, Cola., **Allergy and Asthma Prevention Center, Incorporated Medical Group, San Diego, Calif., and ***Allergy Respiratory Institute of Colorado, Denver, Cola. Received for publication May 12, 1987. Accepted for publication July 29, 1987. Reprint requests: William K. Dolen, MD, MAJ, MC, Allergy-Immunology Service, Fitzsimons Army Medical Ctr., Aurora, CO 80045-5001. The opinions or assertions contained herein are the private views of the authors and are not to be construed as official or as re-flecting the views of the Department of Defense. The protocol under which this study was conducted was approved by the Clinical Research and Human Use Committee of Fitzsimons Army Medical Center and the Office of the Surgeon General. 1Jnited States Army.",
year = "1988",
month = feb,
doi = "10.1016/0091-6749(88)90922-0",
language = "English (US)",
volume = "81",
pages = "489--493",
journal = "Journal of Allergy and Clinical Immunology",
issn = "0091-6749",
publisher = "Mosby Inc.",
number = "2",
}