Comparison of restriction enzyme analysis and pulsed-field gradient gel electrophoresis as typing systems for Candida albicans

Jose Antonio Vazquez, A. Beckley, J. D. Sobel, M. J. Zervos

Research output: Contribution to journalArticle

68 Citations (Scopus)

Abstract

Candida species are an important cause of infection in immunocompromised hosts and the leading cause of nosocomial fungal infections. Study of the epidemiology of Candida infection has been difficult because of lack of a reliable typing system. We describe a typing system utilizing contour-clamped homogeneous electric fields (CHEF), which is a modified version of pulsed-field gradient gel electrophoresis, and compared it with restriction enzyme analysis (REA) of genomic DNA. The study was done with 35 Candida albicans clinical isolates from separate patients. CHEF and REA were performed on each isolate, and the patterns were compared. The REA procedure revealed 17 strain types while the CHEF procedure was able to distinguish 23 strain types of C. albicans. The CHEF technique yields unique patterns of chromosomal bands that can be used to distinguish clinical isolates and demonstrates greater sensitivity than REA.

Original languageEnglish (US)
Pages (from-to)962-967
Number of pages6
JournalJournal of Clinical Microbiology
Volume29
Issue number5
StatePublished - May 24 1991
Externally publishedYes

Fingerprint

Restriction Mapping
Pulsed Field Gel Electrophoresis
Candida albicans
Candida
Mycoses
Immunocompromised Host
Cross Infection
Infection
Epidemiology
DNA

ASJC Scopus subject areas

  • Microbiology (medical)
  • Microbiology

Cite this

Comparison of restriction enzyme analysis and pulsed-field gradient gel electrophoresis as typing systems for Candida albicans. / Vazquez, Jose Antonio; Beckley, A.; Sobel, J. D.; Zervos, M. J.

In: Journal of Clinical Microbiology, Vol. 29, No. 5, 24.05.1991, p. 962-967.

Research output: Contribution to journalArticle

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