Comparison of the Accessory Activity of Murine Peritoneal Cavity Macrophage Derived Dendritic Cells and Peritoneal Cavity Macrophages in a Mixed Lymphocyte Reaction

Levi H Makala, Yoshifumi Nishikawa, Takenori Kamada, Hiroshi Suzuki, Xuenan Xuan, Ikuo Igarashi, Hideyuki Nagasawa

Research output: Contribution to journalArticle

6 Scopus citations


A detailed comparison of the accessory cell activities was carried out among murine peritoneal cavity macrophages (PEC-MΦ). peritoneal cavity macrophages stimulated with granulocyte-macrophage colony stimulating factor (GM-CSF) plus interleukin 4 (IL-4), the most popular cytokine combination widely used to generate dendritic cells (DC) and peritoneal cavity macrophage-derived DC (PEC-DC) using a two-way mixed lymphocyte reaction (MLR). All the cell types used efficiently induced statistically significant naïve T cell proliferation at all culture time points and responder:stimulator ratios used. However, marked differences were noted in the magnitude of the proliferative responses. These variations may be attributed to the intensity of expression of MHC class II glycoproteins, as well as the actual numbers of MHC class II+ cells.

Original languageEnglish (US)
Pages (from-to)1271-1277
Number of pages7
JournalJournal of Veterinary Medical Science
Issue number12
StatePublished - Dec 1 2001



  • Mixed lymphocyte reaction
  • Peritoneal cavity macrophage
  • Peritoneal cavity macrophage-derived dendritic cell

ASJC Scopus subject areas

  • veterinary(all)

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