Comparison of the structure of two cardiac troponin T isoforms

V. V. Risnik, Alexander Dmitriyevich Verin, N. B. Gusev

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Two isoforms of troponin T have been isolated from bovine cardiac muscle. One isoform has an M(r) of 31 000 and a pI about 7.1, the corresponding values for the second isoform being 33 000 and 6.5. Both isoforms have identical C- and N-terminal sequences, and, according to the data from tryptic-peptide mapping, a similar structure of the central and C-terminal domains. The large N-terminal peptides of troponin T isoforms differ in the content of glutamine/glutamic acid and alanine. It is concluded that the isoform with M(r) 33 000 has an additional peptide enriched with glutamic acid and alanine that is inserted between the N-terminal pentapeptide and the cysteine located 40-60 residues from the N-terminus.

Original languageEnglish (US)
Pages (from-to)549-552
Number of pages4
JournalBiochemical Journal
Volume225
Issue number2
DOIs
StatePublished - Jan 1 1985

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Troponin T
Protein Isoforms
Alanine
Peptides
Glutamic Acid
Peptide T
Peptide Mapping
Glutamine
Cysteine
Muscle
Myocardium

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Comparison of the structure of two cardiac troponin T isoforms. / Risnik, V. V.; Verin, Alexander Dmitriyevich; Gusev, N. B.

In: Biochemical Journal, Vol. 225, No. 2, 01.01.1985, p. 549-552.

Research output: Contribution to journalArticle

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