Concomitant activation of miR-107/PDCD10 and hypoxamir-210/Casp8ap2 and their role in cytoprotection during ischemic preconditioning of stem cells

Ha Won Kim, Faryal Mallick, Shazia Durrani, Muhammad Ashraf, Shujia Jiang, Khawaja Husnain Haider

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Aims: To establish a functional link between microRNA-107 (miR-107) and stem cell survival during ischemic preconditioning (IPC) of stem cells with multiple cycles of brief anoxia/re-oxygenation (10 or 30min, one to three cycles) and show that the cytoprotective effects were independent of hypoxamir-210. Results: We demonstrated the induction of miR-107 in response to the IPC-induced activation of Akt/hypoxia inducible factor-1α (HIF-1α) in preconditioned mesenchymal stem cells (PCMSC), which showed improved survival during subsequent exposure to 6h of lethal anoxia (p<0.05 vs. non-preconditioned MSC[non-PCMSC]). In silico analysis and luciferase activity assay confirmed programmed cell death-10 (PDCD10) as a putative target of miR-107 in PCMSC, which was significantly reduced during IPC and inversely related to stem cell survival under 6h of lethal anoxia. Loss-of-function studies with miR-107 antagomir showed a significantly reduced survival of PCMSC. A comparison of miR-107 and miR-210 showed that both miRs participated independently via their respective putative target genes Pdcd10 and Casp8ap2. The simultaneous abrogation of Pdcd10 and Casp8ap2 had a stronger effect on PCMSC survival under lethal anoxia. The transplantation of PCMSC in an acute model of myocardial infarction showed a significantly improved survival of transplanted PCMSC with concomitantly enhanced miR-107 expression in PCMSC-transplanted animal hearts. Innovation: Cytoprotection afforded by IPC is regulated by miR-107 induction via Pdcd10 independent of miR-210/Casp8ap2 signaling, and the simultaneous abrogation miR-107/miR-210 has a stronger effect on the loss of PCMSC survival. Conclusion: IPC enhances stem cell survival via the combined participation of hypoxia responsive miRs miR-107 and miR-210 via their respective putative target genes Pdcd10 and Casp8ap2. Antioxid. Redox Signal. 00, 000-000.

Original languageEnglish (US)
Pages (from-to)1053-1065
Number of pages13
JournalAntioxidants and Redox Signaling
Volume17
Issue number8
DOIs
StatePublished - Oct 15 2012

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Ischemic Preconditioning
Cytoprotection
Cell death
Stem cells
MicroRNAs
Cell Death
Stem Cells
Mesenchymal Stromal Cells
Chemical activation
Cell Survival
Mesenchymal Stem Cell Transplantation
Hypoxia-Inducible Factor 1
Genes
Luciferases
Computer Simulation
Oxidation-Reduction
Oxygenation
Myocardial Infarction
Hypoxia
Assays

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Molecular Biology
  • Clinical Biochemistry
  • Cell Biology

Cite this

Concomitant activation of miR-107/PDCD10 and hypoxamir-210/Casp8ap2 and their role in cytoprotection during ischemic preconditioning of stem cells. / Kim, Ha Won; Mallick, Faryal; Durrani, Shazia; Ashraf, Muhammad; Jiang, Shujia; Haider, Khawaja Husnain.

In: Antioxidants and Redox Signaling, Vol. 17, No. 8, 15.10.2012, p. 1053-1065.

Research output: Contribution to journalArticle

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abstract = "Aims: To establish a functional link between microRNA-107 (miR-107) and stem cell survival during ischemic preconditioning (IPC) of stem cells with multiple cycles of brief anoxia/re-oxygenation (10 or 30min, one to three cycles) and show that the cytoprotective effects were independent of hypoxamir-210. Results: We demonstrated the induction of miR-107 in response to the IPC-induced activation of Akt/hypoxia inducible factor-1α (HIF-1α) in preconditioned mesenchymal stem cells (PCMSC), which showed improved survival during subsequent exposure to 6h of lethal anoxia (p<0.05 vs. non-preconditioned MSC[non-PCMSC]). In silico analysis and luciferase activity assay confirmed programmed cell death-10 (PDCD10) as a putative target of miR-107 in PCMSC, which was significantly reduced during IPC and inversely related to stem cell survival under 6h of lethal anoxia. Loss-of-function studies with miR-107 antagomir showed a significantly reduced survival of PCMSC. A comparison of miR-107 and miR-210 showed that both miRs participated independently via their respective putative target genes Pdcd10 and Casp8ap2. The simultaneous abrogation of Pdcd10 and Casp8ap2 had a stronger effect on PCMSC survival under lethal anoxia. The transplantation of PCMSC in an acute model of myocardial infarction showed a significantly improved survival of transplanted PCMSC with concomitantly enhanced miR-107 expression in PCMSC-transplanted animal hearts. Innovation: Cytoprotection afforded by IPC is regulated by miR-107 induction via Pdcd10 independent of miR-210/Casp8ap2 signaling, and the simultaneous abrogation miR-107/miR-210 has a stronger effect on the loss of PCMSC survival. Conclusion: IPC enhances stem cell survival via the combined participation of hypoxia responsive miRs miR-107 and miR-210 via their respective putative target genes Pdcd10 and Casp8ap2. Antioxid. Redox Signal. 00, 000-000.",
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T1 - Concomitant activation of miR-107/PDCD10 and hypoxamir-210/Casp8ap2 and their role in cytoprotection during ischemic preconditioning of stem cells

AU - Kim, Ha Won

AU - Mallick, Faryal

AU - Durrani, Shazia

AU - Ashraf, Muhammad

AU - Jiang, Shujia

AU - Haider, Khawaja Husnain

PY - 2012/10/15

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N2 - Aims: To establish a functional link between microRNA-107 (miR-107) and stem cell survival during ischemic preconditioning (IPC) of stem cells with multiple cycles of brief anoxia/re-oxygenation (10 or 30min, one to three cycles) and show that the cytoprotective effects were independent of hypoxamir-210. Results: We demonstrated the induction of miR-107 in response to the IPC-induced activation of Akt/hypoxia inducible factor-1α (HIF-1α) in preconditioned mesenchymal stem cells (PCMSC), which showed improved survival during subsequent exposure to 6h of lethal anoxia (p<0.05 vs. non-preconditioned MSC[non-PCMSC]). In silico analysis and luciferase activity assay confirmed programmed cell death-10 (PDCD10) as a putative target of miR-107 in PCMSC, which was significantly reduced during IPC and inversely related to stem cell survival under 6h of lethal anoxia. Loss-of-function studies with miR-107 antagomir showed a significantly reduced survival of PCMSC. A comparison of miR-107 and miR-210 showed that both miRs participated independently via their respective putative target genes Pdcd10 and Casp8ap2. The simultaneous abrogation of Pdcd10 and Casp8ap2 had a stronger effect on PCMSC survival under lethal anoxia. The transplantation of PCMSC in an acute model of myocardial infarction showed a significantly improved survival of transplanted PCMSC with concomitantly enhanced miR-107 expression in PCMSC-transplanted animal hearts. Innovation: Cytoprotection afforded by IPC is regulated by miR-107 induction via Pdcd10 independent of miR-210/Casp8ap2 signaling, and the simultaneous abrogation miR-107/miR-210 has a stronger effect on the loss of PCMSC survival. Conclusion: IPC enhances stem cell survival via the combined participation of hypoxia responsive miRs miR-107 and miR-210 via their respective putative target genes Pdcd10 and Casp8ap2. Antioxid. Redox Signal. 00, 000-000.

AB - Aims: To establish a functional link between microRNA-107 (miR-107) and stem cell survival during ischemic preconditioning (IPC) of stem cells with multiple cycles of brief anoxia/re-oxygenation (10 or 30min, one to three cycles) and show that the cytoprotective effects were independent of hypoxamir-210. Results: We demonstrated the induction of miR-107 in response to the IPC-induced activation of Akt/hypoxia inducible factor-1α (HIF-1α) in preconditioned mesenchymal stem cells (PCMSC), which showed improved survival during subsequent exposure to 6h of lethal anoxia (p<0.05 vs. non-preconditioned MSC[non-PCMSC]). In silico analysis and luciferase activity assay confirmed programmed cell death-10 (PDCD10) as a putative target of miR-107 in PCMSC, which was significantly reduced during IPC and inversely related to stem cell survival under 6h of lethal anoxia. Loss-of-function studies with miR-107 antagomir showed a significantly reduced survival of PCMSC. A comparison of miR-107 and miR-210 showed that both miRs participated independently via their respective putative target genes Pdcd10 and Casp8ap2. The simultaneous abrogation of Pdcd10 and Casp8ap2 had a stronger effect on PCMSC survival under lethal anoxia. The transplantation of PCMSC in an acute model of myocardial infarction showed a significantly improved survival of transplanted PCMSC with concomitantly enhanced miR-107 expression in PCMSC-transplanted animal hearts. Innovation: Cytoprotection afforded by IPC is regulated by miR-107 induction via Pdcd10 independent of miR-210/Casp8ap2 signaling, and the simultaneous abrogation miR-107/miR-210 has a stronger effect on the loss of PCMSC survival. Conclusion: IPC enhances stem cell survival via the combined participation of hypoxia responsive miRs miR-107 and miR-210 via their respective putative target genes Pdcd10 and Casp8ap2. Antioxid. Redox Signal. 00, 000-000.

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