Connective tissue changes in early ischemia of porcine myocardium: An ultrastructural study

S. Sato, Muhammad Ashraf, R. W. Millard, H. Fujiwara, A. Schwartz

Research output: Contribution to journalArticle

91 Citations (Scopus)

Abstract

Scanning electron microscopy and transmission electron microscopy were used together with tannic acid and ruthenium-red staining to examine connective tissue damage caused by acute myocardial ischemia for 20, 40 and 120 min in pig hearts. The microsphere blood flow technique revealed that blood flow was approximately 0.02 ml/min/g in inner, middle and outer thirds of the ischemic zone. After 20 min of occlusion of the left anterior descending coronary artery, the collagen network and microfilaments became irregularly arranged. After 40 min of occlusion, ruthenium-red positive glyco-protein material around the collagen fibrils and elastin began to disappear. After 2 h occlusion, the collagen fibrils and microfilaments had separated from the basement membrane. Collagen fibrils, elastic fibers, and microfilaments were broken down and were found in decreased quantities. These results have revealed that the connective tissue remains intact during the first 20 min of coronary occlusion despite zero blood flow and mild cellular changes but does undergo prominent alterations after 40 min of occlusion.

Original languageEnglish (US)
Pages (from-to)261-275
Number of pages15
JournalJournal of molecular and cellular cardiology
Volume15
Issue number4
DOIs
StatePublished - Jan 1 1983
Externally publishedYes

Fingerprint

Connective Tissue
Myocardium
Swine
Collagen
Ischemia
Actin Cytoskeleton
Ruthenium Red
lissamine rhodamine B
Elastic Tissue
Elastin
Tannins
Coronary Occlusion
Transmission Electron Microscopy
Microspheres
Basement Membrane
Electron Scanning Microscopy
Myocardial Ischemia
Coronary Vessels
Staining and Labeling
Proteins

Keywords

  • Collagen
  • Elastic fiber
  • Ischemia
  • Ruthenium red
  • Scanning and transmission electron microscopy
  • Tannic acid

ASJC Scopus subject areas

  • Molecular Biology
  • Cardiology and Cardiovascular Medicine

Cite this

Connective tissue changes in early ischemia of porcine myocardium : An ultrastructural study. / Sato, S.; Ashraf, Muhammad; Millard, R. W.; Fujiwara, H.; Schwartz, A.

In: Journal of molecular and cellular cardiology, Vol. 15, No. 4, 01.01.1983, p. 261-275.

Research output: Contribution to journalArticle

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AU - Fujiwara, H.

AU - Schwartz, A.

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AB - Scanning electron microscopy and transmission electron microscopy were used together with tannic acid and ruthenium-red staining to examine connective tissue damage caused by acute myocardial ischemia for 20, 40 and 120 min in pig hearts. The microsphere blood flow technique revealed that blood flow was approximately 0.02 ml/min/g in inner, middle and outer thirds of the ischemic zone. After 20 min of occlusion of the left anterior descending coronary artery, the collagen network and microfilaments became irregularly arranged. After 40 min of occlusion, ruthenium-red positive glyco-protein material around the collagen fibrils and elastin began to disappear. After 2 h occlusion, the collagen fibrils and microfilaments had separated from the basement membrane. Collagen fibrils, elastic fibers, and microfilaments were broken down and were found in decreased quantities. These results have revealed that the connective tissue remains intact during the first 20 min of coronary occlusion despite zero blood flow and mild cellular changes but does undergo prominent alterations after 40 min of occlusion.

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