Consistent chromosome abnormalities associated with mouse bladder epithelial cell lines transformed in vitro

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37 Scopus citations

Abstract

Nine epithelial tumor cell lines that were derived from inbred C57BL/lcrf-a(t) adult male mouse bladder and were transformed in vitro were cytogenetically analyzed. Most cell lines were near-diploid and showed only minor karyotypic deviations from normal. Tetraploid cell lines showed duplication of chromosome markers, which suggested that they arose originally as near-diploid cell lines with minor karyotypic changes. Chromosome abnormalities were consistent among different cell lines and involved chromosomes #6, #3, and #15. The Y-chromosome was frequently missing. Identical chromosome abnormalities were present in cell lines that arose in control, dimethyl sulfoxide-treated, or 7,12-dimethylbenz[a]anthracene-treated cultures. the most frequent abnormality was the presence of three copies (trisomy) of chromosome 6. Few structural chromosome abnormalities were detected, although a marker chromosome derived from chromosome #3 was present in several cell lines. Two cell lines had excesses of chromosome #15. These results suggest that excesses of individual chromosomes or parts of chromosomes, particularly chromosome 6, may be important in the expression of the malignant phenotype in mouse bladder epithelium.

Original languageEnglish (US)
Pages (from-to)955-961
Number of pages7
JournalJournal of the National Cancer Institute
Volume65
Issue number5
StatePublished - Dec 1 1980
Externally publishedYes

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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