Construction of recipient strain of expression-secretion system in filamentous fungi

L. Liu, Jin Liu, Run Xiang Qiu, Xingguo Zhu, Guo Min Tang

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Glucoamylase overproducing A. niger T21 was mutated by UV mutagensis. An extracellular acid protease-deficient mutant, A. niger T21-201, which produced only 0.76% extracellular acid protease activity of the parent strain, was screened by casein-degradating plate and determination of protease activity. Moreover, the growth properties and the ability to secrete glucoamylase of A. niger T21-201 are identical to these of starting strain T21. The comparison of expression-secretion levels of heterologous gene in A. niger T21-201 and T21 was carried out with bacterial vhb as reporter, the level of expression-secretion of VHb in A. niger T21-201 was 6-7 times higher than that in T21, but the transcriptional levels of vhb gene in both strains were similar revealed by Northern blot. Therefore, it was demonstrated that the deficiency of acid protease of recipient T21-201 has significant effect on the protection of heterologous protein.

Original languageEnglish (US)
Pages (from-to)667-670
Number of pages4
JournalSheng wu gong cheng xue bao = Chinese journal of biotechnology
Volume18
Issue number6
StatePublished - Jan 1 2002
Externally publishedYes

Fingerprint

Peptide Hydrolases
Fungi
Glucan 1,4-alpha-Glucosidase
Acids
Caseins
Northern Blotting
Genes
Growth
Proteins

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

Cite this

Construction of recipient strain of expression-secretion system in filamentous fungi. / Liu, L.; Liu, Jin; Qiu, Run Xiang; Zhu, Xingguo; Tang, Guo Min.

In: Sheng wu gong cheng xue bao = Chinese journal of biotechnology, Vol. 18, No. 6, 01.01.2002, p. 667-670.

Research output: Contribution to journalArticle

@article{1ddce04b3e064f68929299fecb530562,
title = "Construction of recipient strain of expression-secretion system in filamentous fungi",
abstract = "Glucoamylase overproducing A. niger T21 was mutated by UV mutagensis. An extracellular acid protease-deficient mutant, A. niger T21-201, which produced only 0.76{\%} extracellular acid protease activity of the parent strain, was screened by casein-degradating plate and determination of protease activity. Moreover, the growth properties and the ability to secrete glucoamylase of A. niger T21-201 are identical to these of starting strain T21. The comparison of expression-secretion levels of heterologous gene in A. niger T21-201 and T21 was carried out with bacterial vhb as reporter, the level of expression-secretion of VHb in A. niger T21-201 was 6-7 times higher than that in T21, but the transcriptional levels of vhb gene in both strains were similar revealed by Northern blot. Therefore, it was demonstrated that the deficiency of acid protease of recipient T21-201 has significant effect on the protection of heterologous protein.",
author = "L. Liu and Jin Liu and Qiu, {Run Xiang} and Xingguo Zhu and Tang, {Guo Min}",
year = "2002",
month = "1",
day = "1",
language = "English (US)",
volume = "18",
pages = "667--670",
journal = "Shengwu Gongcheng Xuebao/Chinese Journal of Biotechnology",
issn = "1000-3061",
publisher = "Chinese Academy of Sciences",
number = "6",

}

TY - JOUR

T1 - Construction of recipient strain of expression-secretion system in filamentous fungi

AU - Liu, L.

AU - Liu, Jin

AU - Qiu, Run Xiang

AU - Zhu, Xingguo

AU - Tang, Guo Min

PY - 2002/1/1

Y1 - 2002/1/1

N2 - Glucoamylase overproducing A. niger T21 was mutated by UV mutagensis. An extracellular acid protease-deficient mutant, A. niger T21-201, which produced only 0.76% extracellular acid protease activity of the parent strain, was screened by casein-degradating plate and determination of protease activity. Moreover, the growth properties and the ability to secrete glucoamylase of A. niger T21-201 are identical to these of starting strain T21. The comparison of expression-secretion levels of heterologous gene in A. niger T21-201 and T21 was carried out with bacterial vhb as reporter, the level of expression-secretion of VHb in A. niger T21-201 was 6-7 times higher than that in T21, but the transcriptional levels of vhb gene in both strains were similar revealed by Northern blot. Therefore, it was demonstrated that the deficiency of acid protease of recipient T21-201 has significant effect on the protection of heterologous protein.

AB - Glucoamylase overproducing A. niger T21 was mutated by UV mutagensis. An extracellular acid protease-deficient mutant, A. niger T21-201, which produced only 0.76% extracellular acid protease activity of the parent strain, was screened by casein-degradating plate and determination of protease activity. Moreover, the growth properties and the ability to secrete glucoamylase of A. niger T21-201 are identical to these of starting strain T21. The comparison of expression-secretion levels of heterologous gene in A. niger T21-201 and T21 was carried out with bacterial vhb as reporter, the level of expression-secretion of VHb in A. niger T21-201 was 6-7 times higher than that in T21, but the transcriptional levels of vhb gene in both strains were similar revealed by Northern blot. Therefore, it was demonstrated that the deficiency of acid protease of recipient T21-201 has significant effect on the protection of heterologous protein.

UR - http://www.scopus.com/inward/record.url?scp=0038505330&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0038505330&partnerID=8YFLogxK

M3 - Article

VL - 18

SP - 667

EP - 670

JO - Shengwu Gongcheng Xuebao/Chinese Journal of Biotechnology

JF - Shengwu Gongcheng Xuebao/Chinese Journal of Biotechnology

SN - 1000-3061

IS - 6

ER -