Abstract
Inhibition of nitric oxide generation with Nw‐nitro‐l‐arginine (nitroarginine) reduced vasodilator responses to bradykinin and acetylcholine and enhanced those to nitroprusside in the rat isolated perfused kidney, preconstricted with phenylephrine. Inhibition of cyclo‐oxygenase with indomethacin, decreased the vasodilator responses to bradykinin by ∼25% without affecting those to acetylcholine or nitroprusside. BW755c, a dual inhibitor of cyclo‐oxygenase and lipoxygenase, reduced renal vasodilator responses to bradykinin, comparable to the effect of indomethacin suggesting an effect related to inhibition of cyclo‐oxygenase rather than lipoxygenase. ETYA, an inhibitor of all arachidonic acid metabolic pathways, markedly reduced vasodilator responses to bradykinin but was without effect on the renal vasodilatation induced by acetylcholine or nitroprusside. Clotrimazole and 7‐ethoxyresorufin, inhibitors of cytochrome P450, greatly attenuated vasodilator responses to bradykinin without affecting those to acetylcholine or nitroprusside. These data suggest that the renal vasodilator response to bradykinin is subserved by arachidonic acid metabolites as well as nitric oxide, the former accounting for up to 70% of the vasodilator effect of bradykinin. 1992 British Pharmacological Society
| Original language | English (US) |
|---|---|
| Pages (from-to) | 722-725 |
| Number of pages | 4 |
| Journal | British Journal of Pharmacology |
| Volume | 107 |
| Issue number | 3 |
| DOIs | |
| State | Published - Nov 1992 |
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Keywords
- NO synthesis inhibition
- Rat perfused kidney
- bradykinin‐induced vasodilatation
- cytochrome P450 inhibitors
- inhibition of arachidonate metabolism
ASJC Scopus subject areas
- Pharmacology
Cite this
Contribution of NO and cytochrome P450 to the vasodilator effect of bradykinin in the rat kidney. / Fulton, D.; McGiff, J. C.; Quilley, J.
In: British Journal of Pharmacology, Vol. 107, No. 3, 11.1992, p. 722-725.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Contribution of NO and cytochrome P450 to the vasodilator effect of bradykinin in the rat kidney
AU - Fulton, D.
AU - McGiff, J. C.
AU - Quilley, J.
PY - 1992/11
Y1 - 1992/11
N2 - Inhibition of nitric oxide generation with Nw‐nitro‐l‐arginine (nitroarginine) reduced vasodilator responses to bradykinin and acetylcholine and enhanced those to nitroprusside in the rat isolated perfused kidney, preconstricted with phenylephrine. Inhibition of cyclo‐oxygenase with indomethacin, decreased the vasodilator responses to bradykinin by ∼25% without affecting those to acetylcholine or nitroprusside. BW755c, a dual inhibitor of cyclo‐oxygenase and lipoxygenase, reduced renal vasodilator responses to bradykinin, comparable to the effect of indomethacin suggesting an effect related to inhibition of cyclo‐oxygenase rather than lipoxygenase. ETYA, an inhibitor of all arachidonic acid metabolic pathways, markedly reduced vasodilator responses to bradykinin but was without effect on the renal vasodilatation induced by acetylcholine or nitroprusside. Clotrimazole and 7‐ethoxyresorufin, inhibitors of cytochrome P450, greatly attenuated vasodilator responses to bradykinin without affecting those to acetylcholine or nitroprusside. These data suggest that the renal vasodilator response to bradykinin is subserved by arachidonic acid metabolites as well as nitric oxide, the former accounting for up to 70% of the vasodilator effect of bradykinin. 1992 British Pharmacological Society
AB - Inhibition of nitric oxide generation with Nw‐nitro‐l‐arginine (nitroarginine) reduced vasodilator responses to bradykinin and acetylcholine and enhanced those to nitroprusside in the rat isolated perfused kidney, preconstricted with phenylephrine. Inhibition of cyclo‐oxygenase with indomethacin, decreased the vasodilator responses to bradykinin by ∼25% without affecting those to acetylcholine or nitroprusside. BW755c, a dual inhibitor of cyclo‐oxygenase and lipoxygenase, reduced renal vasodilator responses to bradykinin, comparable to the effect of indomethacin suggesting an effect related to inhibition of cyclo‐oxygenase rather than lipoxygenase. ETYA, an inhibitor of all arachidonic acid metabolic pathways, markedly reduced vasodilator responses to bradykinin but was without effect on the renal vasodilatation induced by acetylcholine or nitroprusside. Clotrimazole and 7‐ethoxyresorufin, inhibitors of cytochrome P450, greatly attenuated vasodilator responses to bradykinin without affecting those to acetylcholine or nitroprusside. These data suggest that the renal vasodilator response to bradykinin is subserved by arachidonic acid metabolites as well as nitric oxide, the former accounting for up to 70% of the vasodilator effect of bradykinin. 1992 British Pharmacological Society
KW - NO synthesis inhibition
KW - Rat perfused kidney
KW - bradykinin‐induced vasodilatation
KW - cytochrome P450 inhibitors
KW - inhibition of arachidonate metabolism
UR - http://www.scopus.com/inward/record.url?scp=0026793950&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0026793950&partnerID=8YFLogxK
U2 - 10.1111/j.1476-5381.1992.tb14513.x
DO - 10.1111/j.1476-5381.1992.tb14513.x
M3 - Article
C2 - 1472970
AN - SCOPUS:0026793950
VL - 107
SP - 722
EP - 725
JO - British Journal of Pharmacology
JF - British Journal of Pharmacology
SN - 0007-1188
IS - 3
ER -