Correction to: Extracellular DNA: A Missing Link in the Pathogenesis of Ectopic Mineralization (Advanced Science, (2022), 9, 5, (2103693), 10.1002/advs.202103693)

Adv. Sci. 2022, 9, 2103693 DOI: 10.1002/advs.202103693 In the originally published version of article, there was an error in Figure 2. The correct Figure 2 can be found below. The authors apologize for any inconvenience this may have caused. 2 Figure (Figure presented.) Correlation of extracellular DNA deposition with ECM calcification of osteoblast-like MC3T3-E1 cells cultured in osteogenic medium in vitro. a) Scheme of MC3T3-E1 cells cultured in osteogenic medium with or without incorporation of DNase I. b) CLSM images of bone-like nodules formation (white dashed line) in MC3T3-E1 cells for 21 days. Extracsellular DNA deposited at sites of bone-like nodules (bar: 10 µm). TEM image taken from the same specimen showed calcified extracellular matrix (bar: 500 nm). c) Confocal live imaging of mineralized nodules (white dashed line) in MC3T3-E1 cells (bar: 10 µm). Colocalization of extracellular DNA and bone-like nodules are clearly identified in the high magnification images of the blue rectangle in (c) (bar: 2 µm). 3D reconstruction showed extracellular DNA aggregation within the regions of calcification (bar: 20 µm). d,e) Alizarin red S staining (d, bar: 2 mm) and CLSM analysis (e, bar: 50 µm) of mineralized MC3T3-E1 cells cultured in osteogenic medium for 7, 14, and 28 days. f) Immunofluorescence of extracellular nucleic acids was performed on mineralized MC3T3-E1 cells stained with SYTOX Green. The data were analyzed quantitatively. Means ± standard deviations (n = 6), one-way ANOVA. There was increase in extracellular DNA deposition over time. g,h) Alizarin red S staining (g, bar: 2 mm) and CLSM analysis (h, bar: 50 µm) of MC3T3-E1 cells cultured in osteogenic medium containing DNase I. Extracellular DNA was barely observed. i) Semiquantitative analysis of alizarin red S stained particles harvested from the MC3T3-E1 group and the MC3T3-E1-DNase I group. Extracted solution was measured at 405 nm. Means ± standard deviations (n = 3), two-way ANOVA.