Corrigendum

IDO2 is critical for IDO1-mediated T-cell regulation and exerts a non-redundant function in inflammation (International Immunology (2014) 26:7(357-367) DOI: 10.1093/intimm/dxt073)

Richard Metz, Courtney Smith, James B. DuHadaway, Phillip Chandler, Babak Baban, Lauren M.F. Merlo, Elizabeth Pigott, Martin P. Keough, Sonja Rust, Andrew L. Mellor, Laura Mandik-Nayak, Alexander J. Muller, George C. Prendergast

Research output: Contribution to journalComment/debate

Abstract

The authors wish to correct an error discovered by one of us (L.M.F.M.) in the structure of the recombinant Ido2 genetic construct used to create the Ido2 mutant mouse analyzed, however, this correction does not impact its validity as a loss-of-function strain or therefore any conclusions of the study. As illustrated below in the revised Fig. 1, the location of the two loxP sites employed for Cre-mediated excision are located within exons 9 and 10 in the Ido2 gene, rather than upstream of exon 9 and downstream of exon 10 as reported (due to an error in selection of this construct for use for unknown reasons). In this construct, stop codons are generated for Ido2 translation by frameshift readthroughs at the loxP cassette which was inserted into exon 9 (top line of the figure). Accordingly, targeted insertion into the mouse genome created a constitutive loss-of-function allele without further utility as a cre-inducible loss-of-function allele. In breeding the parental strain to EII-cre mice, to induce cre-mediated recombination, we generated a systemic deletion mutant lacking the exon 9-10 segment (bottom line in the figure). Thus, a valid loss-of-function mutant was created, albeit one derived from a mutant rather than wild-type parent. The corrected genetic construct eliminates uses for cre-inducible deletion, e.g. to generate tissue-specific mutants, but the strain that was created nevertheless remains valid and useful as a systemic deletion mutant.

Original languageEnglish (US)
Pages (from-to)181-182
Number of pages2
JournalInternational Immunology
Volume31
Issue number3
DOIs
StatePublished - Mar 5 2019

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Allergy and Immunology
Exons
Inflammation
T-Lymphocytes
Alleles
Terminator Codon
Genetic Recombination
Breeding
corrigendum
Genome
Genes

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Corrigendum : IDO2 is critical for IDO1-mediated T-cell regulation and exerts a non-redundant function in inflammation (International Immunology (2014) 26:7(357-367) DOI: 10.1093/intimm/dxt073). / Metz, Richard; Smith, Courtney; DuHadaway, James B.; Chandler, Phillip; Baban, Babak; Merlo, Lauren M.F.; Pigott, Elizabeth; Keough, Martin P.; Rust, Sonja; Mellor, Andrew L.; Mandik-Nayak, Laura; Muller, Alexander J.; Prendergast, George C.

In: International Immunology, Vol. 31, No. 3, 05.03.2019, p. 181-182.

Research output: Contribution to journalComment/debate

Metz, R, Smith, C, DuHadaway, JB, Chandler, P, Baban, B, Merlo, LMF, Pigott, E, Keough, MP, Rust, S, Mellor, AL, Mandik-Nayak, L, Muller, AJ & Prendergast, GC 2019, 'Corrigendum: IDO2 is critical for IDO1-mediated T-cell regulation and exerts a non-redundant function in inflammation (International Immunology (2014) 26:7(357-367) DOI: 10.1093/intimm/dxt073)', International Immunology, vol. 31, no. 3, pp. 181-182. https://doi.org/10.1093/intimm/dxz003
Metz, Richard ; Smith, Courtney ; DuHadaway, James B. ; Chandler, Phillip ; Baban, Babak ; Merlo, Lauren M.F. ; Pigott, Elizabeth ; Keough, Martin P. ; Rust, Sonja ; Mellor, Andrew L. ; Mandik-Nayak, Laura ; Muller, Alexander J. ; Prendergast, George C. / Corrigendum : IDO2 is critical for IDO1-mediated T-cell regulation and exerts a non-redundant function in inflammation (International Immunology (2014) 26:7(357-367) DOI: 10.1093/intimm/dxt073). In: International Immunology. 2019 ; Vol. 31, No. 3. pp. 181-182.
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abstract = "The authors wish to correct an error discovered by one of us (L.M.F.M.) in the structure of the recombinant Ido2 genetic construct used to create the Ido2 mutant mouse analyzed, however, this correction does not impact its validity as a loss-of-function strain or therefore any conclusions of the study. As illustrated below in the revised Fig. 1, the location of the two loxP sites employed for Cre-mediated excision are located within exons 9 and 10 in the Ido2 gene, rather than upstream of exon 9 and downstream of exon 10 as reported (due to an error in selection of this construct for use for unknown reasons). In this construct, stop codons are generated for Ido2 translation by frameshift readthroughs at the loxP cassette which was inserted into exon 9 (top line of the figure). Accordingly, targeted insertion into the mouse genome created a constitutive loss-of-function allele without further utility as a cre-inducible loss-of-function allele. In breeding the parental strain to EII-cre mice, to induce cre-mediated recombination, we generated a systemic deletion mutant lacking the exon 9-10 segment (bottom line in the figure). Thus, a valid loss-of-function mutant was created, albeit one derived from a mutant rather than wild-type parent. The corrected genetic construct eliminates uses for cre-inducible deletion, e.g. to generate tissue-specific mutants, but the strain that was created nevertheless remains valid and useful as a systemic deletion mutant.",
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AU - Metz, Richard

AU - Smith, Courtney

AU - DuHadaway, James B.

AU - Chandler, Phillip

AU - Baban, Babak

AU - Merlo, Lauren M.F.

AU - Pigott, Elizabeth

AU - Keough, Martin P.

AU - Rust, Sonja

AU - Mellor, Andrew L.

AU - Mandik-Nayak, Laura

AU - Muller, Alexander J.

AU - Prendergast, George C.

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N2 - The authors wish to correct an error discovered by one of us (L.M.F.M.) in the structure of the recombinant Ido2 genetic construct used to create the Ido2 mutant mouse analyzed, however, this correction does not impact its validity as a loss-of-function strain or therefore any conclusions of the study. As illustrated below in the revised Fig. 1, the location of the two loxP sites employed for Cre-mediated excision are located within exons 9 and 10 in the Ido2 gene, rather than upstream of exon 9 and downstream of exon 10 as reported (due to an error in selection of this construct for use for unknown reasons). In this construct, stop codons are generated for Ido2 translation by frameshift readthroughs at the loxP cassette which was inserted into exon 9 (top line of the figure). Accordingly, targeted insertion into the mouse genome created a constitutive loss-of-function allele without further utility as a cre-inducible loss-of-function allele. In breeding the parental strain to EII-cre mice, to induce cre-mediated recombination, we generated a systemic deletion mutant lacking the exon 9-10 segment (bottom line in the figure). Thus, a valid loss-of-function mutant was created, albeit one derived from a mutant rather than wild-type parent. The corrected genetic construct eliminates uses for cre-inducible deletion, e.g. to generate tissue-specific mutants, but the strain that was created nevertheless remains valid and useful as a systemic deletion mutant.

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