Coupling of cell energetics with membrane metabolic sensing: Integrative signaling through creatine kinase phosphotransfer disrupted by M-CK gene knock-out

M. Roselle Abraham, Vitaliy A. Selivanov, Denice M. Hodgson, Darko Pucar, Leonid V. Zingman, Be Wieringa, Petras P. Dzeja, Alexey E. Alekseev, Andre Terzic

Research output: Contribution to journalArticle

126 Citations (Scopus)

Abstract

Transduction of metabolic signals is essential in preserving cellular homeostasis. Yet, principles governing integration and synchronization of membrane metabolic sensors with cell metabolism remain elusive. Here, analysis of cellular nucleotide fluxes and nucleotide-dependent gating of the ATP-sensitive K+ (KATP) channel, a prototypic metabolic sensor, revealed a diffusional barrier within the submembrane space, preventing direct reception of cytosolic signals. Creatine kinase phosphotransfer, captured by 18O-assisted 31P NMR, coordinated tightly with ATP turnover, reflecting the cellular energetic status. The dynamics of high energy phosphoryl transfer through the creatine kinase relay permitted a high fidelity transmission of energetic signals into the submembrane compartment synchronizing KATP channel activity with cell metabolism. Knock-out of the creatine kinase M-CK gene disrupted signal delivery to KATP channels and generated a cellular phenotype with increased electrical vulnerability. Thus, in the compartmentalized cell environment, phosphotransfer systems shunt diffusional barriers and secure regimented signal transduction integrating metabolic sensors with the cellular energetic network.

Original languageEnglish (US)
Pages (from-to)24427-24434
Number of pages8
JournalJournal of Biological Chemistry
Volume277
Issue number27
DOIs
StatePublished - Jul 5 2002

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KATP Channels
Gene Knockout Techniques
Creatine Kinase
Genes
Membranes
Metabolism
Signal Transduction
Sensors
Nucleotides
Adenosine Triphosphate
MM Form Creatine Kinase
Signal transduction
Energy Transfer
Energy transfer
Synchronization
Homeostasis
Nuclear magnetic resonance
Fluxes
Phenotype

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Coupling of cell energetics with membrane metabolic sensing : Integrative signaling through creatine kinase phosphotransfer disrupted by M-CK gene knock-out. / Roselle Abraham, M.; Selivanov, Vitaliy A.; Hodgson, Denice M.; Pucar, Darko; Zingman, Leonid V.; Wieringa, Be; Dzeja, Petras P.; Alekseev, Alexey E.; Terzic, Andre.

In: Journal of Biological Chemistry, Vol. 277, No. 27, 05.07.2002, p. 24427-24434.

Research output: Contribution to journalArticle

Roselle Abraham, M, Selivanov, VA, Hodgson, DM, Pucar, D, Zingman, LV, Wieringa, B, Dzeja, PP, Alekseev, AE & Terzic, A 2002, 'Coupling of cell energetics with membrane metabolic sensing: Integrative signaling through creatine kinase phosphotransfer disrupted by M-CK gene knock-out', Journal of Biological Chemistry, vol. 277, no. 27, pp. 24427-24434. https://doi.org/10.1074/jbc.M201777200
Roselle Abraham, M. ; Selivanov, Vitaliy A. ; Hodgson, Denice M. ; Pucar, Darko ; Zingman, Leonid V. ; Wieringa, Be ; Dzeja, Petras P. ; Alekseev, Alexey E. ; Terzic, Andre. / Coupling of cell energetics with membrane metabolic sensing : Integrative signaling through creatine kinase phosphotransfer disrupted by M-CK gene knock-out. In: Journal of Biological Chemistry. 2002 ; Vol. 277, No. 27. pp. 24427-24434.
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