CREB DNA binding activation by a 50-Hz magnetic field in HL60 cells is dependent on extra- and intracellular Ca2+ but not PKA, PKC, ERK, or p38 MAPK

Jiliang Zhou, Gengdong Yao, Jingsong Zhang, Zongliang Chang

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41 Scopus citations


To investigate the possible mechanism of gene transcription changes induced by magnetic field (MF), we examined the DNA binding behavior of the transcription factor cyclic-AMP responsive element binding protein (CREB) in HL60 cells after exposure to a 0.1 mT 50-Hz extremely low frequency (ELF) sinusoidal MF by a gel shift assay. Magnetic field induced a time-dependent activation of CREB binding. The complex formation increased shortly after MF exposure for 10 min, reaching a peak level after 1 h, and then recovered to basal level at 4 h after exposure. A novel MF-induced ATF2/ATF2 homodimer formation was observed after MF exposure for 30 min, 1, and 2 h. Furthermore, We found that the MF-induced increase of CREB DNA binding in HL60 cells was dependent on both extracellular and intracellular Ca2+ but not PKA, PKC, ERK, or p38 MAPK by using various pathway inhibitors. These data indicate that MF exposure activates CREB DNA binding through calcium-related signal transduction pathways under our experimental conditions.

Original languageEnglish (US)
Pages (from-to)1013-1018
Number of pages6
JournalBiochemical and Biophysical Research Communications
Issue number4
Publication statusPublished - Oct 14 2002



  • CREB
  • Calcium
  • Magnetic field

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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