CREB DNA binding activation by a 50-Hz magnetic field in HL60 cells is dependent on extra- and intracellular Ca2+ but not PKA, PKC, ERK, or p38 MAPK

Jiliang Zhou, Gengdong Yao, Jingsong Zhang, Zongliang Chang

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

To investigate the possible mechanism of gene transcription changes induced by magnetic field (MF), we examined the DNA binding behavior of the transcription factor cyclic-AMP responsive element binding protein (CREB) in HL60 cells after exposure to a 0.1 mT 50-Hz extremely low frequency (ELF) sinusoidal MF by a gel shift assay. Magnetic field induced a time-dependent activation of CREB binding. The complex formation increased shortly after MF exposure for 10 min, reaching a peak level after 1 h, and then recovered to basal level at 4 h after exposure. A novel MF-induced ATF2/ATF2 homodimer formation was observed after MF exposure for 30 min, 1, and 2 h. Furthermore, We found that the MF-induced increase of CREB DNA binding in HL60 cells was dependent on both extracellular and intracellular Ca2+ but not PKA, PKC, ERK, or p38 MAPK by using various pathway inhibitors. These data indicate that MF exposure activates CREB DNA binding through calcium-related signal transduction pathways under our experimental conditions.

Original languageEnglish (US)
Pages (from-to)1013-1018
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume296
Issue number4
DOIs
StatePublished - Oct 14 2002

Fingerprint

HL-60 Cells
DNA-Binding Proteins
p38 Mitogen-Activated Protein Kinases
Magnetic Fields
Protein Binding
Cyclic AMP
Carrier Proteins
Chemical activation
Magnetic fields
Signal transduction
Transcription
Assays
Signal Transduction
Transcription Factors
Genes
Gels
Calcium
DNA

Keywords

  • CREB
  • Calcium
  • Magnetic field

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

CREB DNA binding activation by a 50-Hz magnetic field in HL60 cells is dependent on extra- and intracellular Ca2+ but not PKA, PKC, ERK, or p38 MAPK. / Zhou, Jiliang; Yao, Gengdong; Zhang, Jingsong; Chang, Zongliang.

In: Biochemical and Biophysical Research Communications, Vol. 296, No. 4, 14.10.2002, p. 1013-1018.

Research output: Contribution to journalArticle

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